PMID- 31739606
OWN - NLM
STAT- MEDLINE
DCOM- 20200916
LR  - 20200916
IS  - 1999-4915 (Electronic)
IS  - 1999-4915 (Linking)
VI  - 11
IP  - 11
DP  - 2019 Nov 14
TI  - The U3 and Env Proteins of Jaagsiekte Sheep Retrovirus and Enzootic Nasal Tumor 
      Virus Both Contribute to Tissue Tropism.
LID - 10.3390/v11111061 [doi]
LID - 1061
AB  - Jaagsiekte sheep retrovirus (JSRV) and enzootic nasal tumor virus (ENTV) are 
      small-ruminant betaretroviruses that share high nucleotide and amino acid 
      identity, utilize the same cellular receptor, hyaluronoglucosaminidase 2 (Hyal2) 
      for entry, and transform tissues with their envelope (Env) glycoprotein; yet, 
      they target discrete regions of the respiratory tract-the lung and nose, 
      respectively. This distinct tissue selectivity makes them ideal tools with which 
      to study the pathogenesis of betaretroviruses. To uncover the genetic 
      determinants of tropism, we constructed JSRV-ENTV chimeric viruses and produced 
      lentivectors pseudotyped with the Env proteins from JSRV (Jenv) and ENTV (Eenv). 
      Through the transduction and infection of lung and nasal turbinate tissue slices, 
      we observed that Hyal2 expression levels strongly influence ENTV entry, but that 
      the long terminal repeat (LTR) promoters of these viruses are likely responsible 
      for tissue-specificity. Furthermore, we show evidence of ENTV Env expression in 
      chondrocytes within ENTV-infected nasal turbinate tissue, where Hyal2 is highly 
      expressed. Our work suggests that the unique tissue tropism of JSRV and ENTV 
      stems from the combined effort of the envelope glycoprotein-receptor interactions 
      and the LTR and provides new insight into the pathogenesis of ENTV.
FAU - Rosales Gerpe, Maria C
AU  - Rosales Gerpe MC
AD  - Department of Pathobiology, Ontario Veterinary College, University of Guelph, 
      Guelph ON N1G 2W1, Ontario, Canada.
FAU - van Lieshout, Laura P
AU  - van Lieshout LP
AD  - Department of Pathobiology, Ontario Veterinary College, University of Guelph, 
      Guelph ON N1G 2W1, Ontario, Canada.
FAU - Domm, Jakob M
AU  - Domm JM
AD  - Department of Pathobiology, Ontario Veterinary College, University of Guelph, 
      Guelph ON N1G 2W1, Ontario, Canada.
FAU - Ingrao, Joelle C
AU  - Ingrao JC
AD  - Department of Pathobiology, Ontario Veterinary College, University of Guelph, 
      Guelph ON N1G 2W1, Ontario, Canada.
FAU - Datu, Jodre
AU  - Datu J
AD  - Department of Pathobiology, Ontario Veterinary College, University of Guelph, 
      Guelph ON N1G 2W1, Ontario, Canada.
FAU - Walsh, Scott R
AU  - Walsh SR
AD  - McMaster Immunology Research Center, Department of Pathology and Molecular 
      Medicine, McMaster University, Hamilton ON L8S 4L8, Ontario, Canada.
FAU - Yu, Darrick L
AU  - Yu DL
AD  - Department of Pathobiology, Ontario Veterinary College, University of Guelph, 
      Guelph ON N1G 2W1, Ontario, Canada.
FAU - Jong, Jondavid de
AU  - Jong J
AD  - Department of Pathobiology, Ontario Veterinary College, University of Guelph, 
      Guelph ON N1G 2W1, Ontario, Canada.
FAU - Krell, Peter J
AU  - Krell PJ
AD  - Department of Cellular and Molecular Biology, University of Guelph, Guelph ON N1G 
      2W1, Ontario, Canada.
FAU - Wootton, Sarah K
AU  - Wootton SK
AD  - Department of Pathobiology, Ontario Veterinary College, University of Guelph, 
      Guelph ON N1G 2W1, Ontario, Canada.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191114
PL  - Switzerland
TA  - Viruses
JT  - Viruses
JID - 101509722
RN  - 0 (Gene Products, env)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Gene Order
MH  - Gene Products, env/*genetics
MH  - Genome, Viral
MH  - Host Specificity
MH  - Host-Pathogen Interactions
MH  - Humans
MH  - Jaagsiekte sheep retrovirus/*physiology
MH  - Oncogenic Viruses/*physiology
MH  - Pulmonary Adenomatosis, Ovine/*virology
MH  - Reassortant Viruses/genetics
MH  - Sheep
MH  - *Terminal Repeat Sequences
MH  - Tumor Virus Infections/*virology
MH  - *Viral Tropism
PMC - PMC6893448
OTO - NOTNLM
OT  - chimeric virus
OT  - envelope glycoproteins
OT  - long terminal repeat (LTR), sheep betaretroviruses
OT  - pathogenesis
OT  - pseudotyping
OT  - tropism
COIS- The authors declare no conflict of interest. The funders had no role in the 
      design of the study; in the collection, analyses, or interpretation of data; in 
      the writing of the manuscript, or in the decision to publish the results.
EDAT- 2019/11/20 06:00
MHDA- 2020/09/17 06:00
PMCR- 2019/11/01
CRDT- 2019/11/20 06:00
PHST- 2019/10/25 00:00 [received]
PHST- 2019/11/12 00:00 [revised]
PHST- 2019/11/12 00:00 [accepted]
PHST- 2019/11/20 06:00 [entrez]
PHST- 2019/11/20 06:00 [pubmed]
PHST- 2020/09/17 06:00 [medline]
PHST- 2019/11/01 00:00 [pmc-release]
AID - v11111061 [pii]
AID - viruses-11-01061 [pii]
AID - 10.3390/v11111061 [doi]
PST - epublish
SO  - Viruses. 2019 Nov 14;11(11):1061. doi: 10.3390/v11111061.