PMID- 31741734
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231019
IS  - 2048-7177 (Print)
IS  - 2048-7177 (Electronic)
IS  - 2048-7177 (Linking)
VI  - 7
IP  - 11
DP  - 2019 Nov
TI  - Polyethylene glycol-based ultrasonic-assisted enzymatic extraction, 
      characterization, and antioxidant activity in vitro and in vivo of 
      polysaccharides from Lonicerae japonica leaves.
PG  - 3452-3462
LID - 10.1002/fsn3.1186 [doi]
AB  - In this paper, polyethylene glycol (PEG)-based ultrasonic-assisted enzymatic 
      extraction (UAEE) was employed in polysaccharides extraction from Lonicerae 
      japonica leaves (LJLP). The optimal extraction conditions (extraction time of 
      33 min, PEG concentration of 30%, and ultrasonic power of 191 W) were obtained by 
      Box-Behnken design (BBD). Under this condition, the LJLP yield was 14.76%. 
      Furthermore, LJLP was identified as a typical heteropolysaccharide considering 
      the main constitutive monosaccharides include galactose (32.3%), glucose (20.9%), 
      and ribose (15.2%). Moreover, LJLP exhibited high total reducing power and 
      considerable scavenging activities on superoxide radicals, in a 
      concentration-dependent manner in vitro. In addition, antioxidant analysis in 
      vivo revealed that for the LJLP-treated mice, the superoxide dismutase (SOD), 
      glutathione peroxidase (GSH-Px), catalase (CAT) activities, and total antioxidant 
      capacity (TAOC) were significantly increased, while the level of malondialdehyde 
      (MDA) was decreased in both serum and liver.
CI  - (c) 2019 The Authors. Food Science & Nutrition published by Wiley Periodicals, Inc.
FAU - Wu, Wei
AU  - Wu W
AUID- ORCID: 0000-0002-6291-1438
AD  - Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources 
      Comprehensive Utilization Hubei Collaborative Innovation Center for the 
      Characteristic Resources Exploitation of Dabie Mountains Huanggang Normal 
      University Huangzhou China.
FAU - Huang, Tingrong
AU  - Huang T
AD  - Edong Healthcare City Hospital of Traditional Chinese Medicine (Infectious 
      Disease Hospital) Huangshi China.
FAU - Xiang, Fu
AU  - Xiang F
AD  - Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources 
      Comprehensive Utilization Hubei Collaborative Innovation Center for the 
      Characteristic Resources Exploitation of Dabie Mountains Huanggang Normal 
      University Huangzhou China.
LA  - eng
PT  - Journal Article
DEP - 20190913
PL  - United States
TA  - Food Sci Nutr
JT  - Food science & nutrition
JID - 101605473
PMC - PMC6848850
OTO - NOTNLM
OT  - Lonicerae japonica leaves
OT  - PEG-based ultrasonic-assisted enzymatic extraction
OT  - antioxidant activity
OT  - polysaccharides
COIS- The authors declare no conflict of interest.
EDAT- 2019/11/20 06:00
MHDA- 2019/11/20 06:01
PMCR- 2019/09/13
CRDT- 2019/11/20 06:00
PHST- 2019/05/15 00:00 [received]
PHST- 2019/07/27 00:00 [accepted]
PHST- 2019/11/20 06:00 [entrez]
PHST- 2019/11/20 06:00 [pubmed]
PHST- 2019/11/20 06:01 [medline]
PHST- 2019/09/13 00:00 [pmc-release]
AID - FSN31186 [pii]
AID - 10.1002/fsn3.1186 [doi]
PST - epublish
SO  - Food Sci Nutr. 2019 Sep 13;7(11):3452-3462. doi: 10.1002/fsn3.1186. eCollection 
      2019 Nov.