PMID- 31742290
OWN - NLM
STAT- MEDLINE
DCOM- 20200410
LR  - 20200410
IS  - 2042-650X (Electronic)
IS  - 2042-6496 (Linking)
VI  - 10
IP  - 12
DP  - 2019 Dec 11
TI  - Protective effect of Bacillus amyloliquefaciens against Salmonella via polarizing 
      macrophages to M1 phenotype directly and to M2 depended on microbiota.
PG  - 7653-7666
LID - 10.1039/c9fo01651a [doi]
AB  - Bacillus amyloliquefaciens SC06 (BaSC06), a potential probiotic, plays a positive 
      role in animal growth performance and immune function. The aim of the present 
      study was to investigate the protective effect of BaSC06 against Salmonella 
      infection and its association with macrophage polarization. C57BL/6 mice were fed 
      with or without a BaSC06-containing diet before Salmonella enterica Typhimurium 
      (ST) challenge. Results showed that BaSC06 had a protective effect against ST 
      inoculation and induced both M1 and M2 macrophage polarization in the cecum. An 
      in vitro co-culture model demonstrated that BaSC06 promoted M1 polarization 
      directly, and thus increased the phagocytosis and bactericidal activity against 
      ST. In addition, adoptive transfer of bone marrow-derived macrophages (BMDMs) 
      stimulated by BaSC06 significantly decreased the counts of ST in the spleen. 
      Furthermore, 16S rRNA-based analysis of cecal content showed that BaSC06 
      significantly increased the proportion of Verrucomicrobia and decreased 
      Bacterodetes. Transplantation of the fecal microbiota from BaSC06-treated animals 
      promoted M2 macrophage polarization in the cecum and significantly relieved 
      inflammation caused by ST. In conclusion, BaSC06 polarized macrophages to the M1 
      type directly resulting in excellent bactericidal activity. Meanwhile, the 
      microbiota modified by BaSC06 can induce M2 polarization which ameliorates the 
      inflammation caused by ST.
FAU - Fu, Aikun
AU  - Fu A
AD  - Key Laboratory of Animal Molecular Nutrition of Education of Ministry, College of 
      Animal Sciences, Zhejiang University, Hangzhou, China. wfli@zju.edu.cn.
FAU - Mo, Qiufen
AU  - Mo Q
FAU - Wu, Yanping
AU  - Wu Y
FAU - Wang, Baikui
AU  - Wang B
FAU - Liu, Rongrong
AU  - Liu R
FAU - Tang, Li
AU  - Tang L
FAU - Zeng, Zhonghua
AU  - Zeng Z
FAU - Zhang, Xiaoping
AU  - Zhang X
FAU - Li, Weifen
AU  - Li W
LA  - eng
PT  - Journal Article
PL  - England
TA  - Food Funct
JT  - Food & function
JID - 101549033
SB  - IM
MH  - Animals
MH  - Bacillus amyloliquefaciens/*physiology
MH  - Bacteria/classification/genetics/isolation & purification
MH  - Cell Polarity/drug effects
MH  - Humans
MH  - Macrophage Activation/drug effects
MH  - Macrophages/drug effects/*immunology
MH  - Male
MH  - Mice, Inbred C57BL
MH  - *Microbiota/drug effects
MH  - Phagocytosis/drug effects
MH  - Probiotics/*administration & dosage
MH  - Salmonella Infections/immunology/microbiology/*prevention & control
MH  - Salmonella typhimurium/*drug effects/physiology
EDAT- 2019/11/20 06:00
MHDA- 2020/04/11 06:00
CRDT- 2019/11/20 06:00
PHST- 2019/11/20 06:00 [pubmed]
PHST- 2020/04/11 06:00 [medline]
PHST- 2019/11/20 06:00 [entrez]
AID - 10.1039/c9fo01651a [doi]
PST - ppublish
SO  - Food Funct. 2019 Dec 11;10(12):7653-7666. doi: 10.1039/c9fo01651a.