PMID- 31745130
OWN - NLM
STAT- MEDLINE
DCOM- 20201106
LR  - 20210110
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 9
IP  - 1
DP  - 2019 Nov 19
TI  - Field cress genome mapping: Integrating linkage and comparative maps with 
      cytogenetic analysis for rDNA carrying chromosomes.
PG  - 17028
LID - 10.1038/s41598-019-53320-0 [doi]
LID - 17028
AB  - Field cress (Lepidium campestre L.), despite its potential as a sustainable 
      alternative oilseed plant, has been underutilized, and no prior attempts to 
      characterize the genome at the genetic or molecular cytogenetic level have been 
      conducted. Genetic maps are the foundation for anchoring and orienting annotated 
      genome assemblies and positional cloning of candidate genes. Our principal goal 
      was to construct a genetic map using integrated approaches of genetic, 
      comparative and cytogenetic map analyses. In total, 503 F(2) interspecific hybrid 
      individuals were genotyped using 7,624 single nucleotide polymorphism markers. 
      Comparative analysis demonstrated that ~57% of the sequenced loci in L. campestre 
      were congruent with Arabidopsis thaliana (L.) genome and suggested a novel 
      karyotype, which predates the ancestral crucifer karyotype. Aceto-orcein 
      chromosome staining and fluorescence in situ hybridization (FISH) analyses 
      confirmed that L. campestre, L. heterophyllum Benth. and their hybrids had a 
      chromosome number of 2n = 2x = 16. Flow cytometric analysis revealed that both 
      species possess 2C roughly 0.4 picogram DNA. Integrating linkage and comparative 
      maps with cytogenetic map analyses assigned two linkage groups to their 
      particular chromosomes. Future work could incorporate FISH utilizing A. thaliana 
      mapped BAC clones to allow the chromosomes of field cress to be identified 
      reliably.
FAU - Desta, Zeratsion Abera
AU  - Desta ZA
AD  - Department of Plant Breeding, Swedish University of Agricultural Sciences, 
      Sundesvagen 10, Box 101, SE-23053, Alnarp, Sweden. zeratsion.abera@slu.se.
FAU - Kolano, Bozena
AU  - Kolano B
AUID- ORCID: 0000-0002-4187-3807
AD  - Department of Plant Anatomy and Cytology, University of Silesia, Jagiellonska 28, 
      40-032, Katowice, Poland.
FAU - Shamim, Zeeshan
AU  - Shamim Z
AD  - Mirpur University of Science and Technology (MUST), Mirpur AJK, Pakistan.
AD  - School of Biosciences, University of Birmingham, Birmingham, B 15 2TT, United 
      Kingdom.
FAU - Armstrong, Susan J
AU  - Armstrong SJ
AUID- ORCID: 0000-0002-7284-5702
AD  - School of Biosciences, University of Birmingham, Birmingham, B 15 2TT, United 
      Kingdom.
FAU - Rewers, Monika
AU  - Rewers M
AUID- ORCID: 0000-0003-4105-3654
AD  - Laboratory of Molecular Biology and Cytometry, Department of Agricultural 
      Biotechnology, UTP University of Science and Technology, Kaliskiego Ave. 7, 
      85-789, Bydgoszcz, Poland.
FAU - Sliwinska, Elwira
AU  - Sliwinska E
AD  - Laboratory of Molecular Biology and Cytometry, Department of Agricultural 
      Biotechnology, UTP University of Science and Technology, Kaliskiego Ave. 7, 
      85-789, Bydgoszcz, Poland.
FAU - Kushwaha, Sandeep Kumar
AU  - Kushwaha SK
AD  - Department of Plant Breeding, Swedish University of Agricultural Sciences, 
      Sundesvagen 10, Box 101, SE-23053, Alnarp, Sweden.
FAU - Parkin, Isobel A P
AU  - Parkin IAP
AD  - Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, SK, S7N0X2, 
      Canada.
FAU - Ortiz, Rodomiro
AU  - Ortiz R
AD  - Department of Plant Breeding, Swedish University of Agricultural Sciences, 
      Sundesvagen 10, Box 101, SE-23053, Alnarp, Sweden.
FAU - de Koning, Dirk-Jan
AU  - de Koning DJ
AUID- ORCID: 0000-0001-6343-8155
AD  - Department of Animal Breeding and Genetics, Swedish University of Agricultural 
      Sciences, Box 7023, SE 75007, Uppsala, Sweden.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191119
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (DNA, Ribosomal)
SB  - IM
MH  - Arabidopsis/genetics
MH  - Brassicaceae/*genetics
MH  - *Chromosome Mapping
MH  - Computational Biology/methods
MH  - DNA, Ribosomal/genetics
MH  - Genetic Linkage/*genetics
MH  - Genome, Plant/*genetics
MH  - Genomics/methods
MH  - Polymorphism, Single Nucleotide/genetics
PMC - PMC6863836
COIS- The authors declare no competing interests.
EDAT- 2019/11/21 06:00
MHDA- 2020/11/11 06:00
PMCR- 2019/11/19
CRDT- 2019/11/21 06:00
PHST- 2019/05/10 00:00 [received]
PHST- 2019/10/30 00:00 [accepted]
PHST- 2019/11/21 06:00 [entrez]
PHST- 2019/11/21 06:00 [pubmed]
PHST- 2020/11/11 06:00 [medline]
PHST- 2019/11/19 00:00 [pmc-release]
AID - 10.1038/s41598-019-53320-0 [pii]
AID - 53320 [pii]
AID - 10.1038/s41598-019-53320-0 [doi]
PST - epublish
SO  - Sci Rep. 2019 Nov 19;9(1):17028. doi: 10.1038/s41598-019-53320-0.