PMID- 31746381
OWN - NLM
STAT- MEDLINE
DCOM- 20201026
LR  - 20211008
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Linking)
VI  - 21
IP  - 1
DP  - 2020 Jan
TI  - IL‑37 expression is decreased in patients with hyperhomocysteinemia and protects 
      cells from inflammatory injury by homocysteine.
PG  - 371-378
LID - 10.3892/mmr.2019.10804 [doi]
AB  - As a novel anti‑inflammatory cytokine of the interleukin (IL)‑1 family, IL‑37 
      protects the human body from diseases characterized by excessive inflammation. 
      The pathologic process of hyperhomocysteinemia (hHcy) is accompanied by 
      persistent inflammation. However, little is known regarding the role of IL‑37 in 
      hHcy. In the present study, the levels of cytokines including IL‑37, IL‑1beta, IL‑6 
      and tumor necrosis factor‑alpha in the supernatant were detected by ELISA. mRNA and 
      protein expression were detected by Reverse transcription‑quantitative PCR and 
      western blotting, respectively. LDH level was determined by ELISA and the cell 
      viability was detected through CCK‑8 kit. In the present study, mean serum IL‑37 
      levels of patients with hHcy were 32.3% lower than those of controls (P<0.01). In 
      peripheral blood mononuclear cells (PBMCs) from patients with hHcy, mean IL‑37 
      mRNA expression was 73.5% lower (P<0.01) and IL‑37 protein expression was 77.7% 
      lower compared with that of healthy controls (P<0.01). Furthermore, the results 
      demonstrated that exogenous homocysteine (Hcy) stimulation markedly downregulated 
      the mRNA and protein expression levels of IL‑37 in PBMCs in vitro. In 293T cells, 
      overexpression of IL‑37 restored the cell viability impaired by Hcy, and reduced 
      the release of lactate dehydrogenase and the proinflammatory cytokines IL‑1beta, 
      IL‑6 and tumor necrosis factor‑alpha. In conclusion, IL‑37 was downregulated by Hcy 
      in vivo and in vitro, and IL‑37 exhibited a protective role against cell injury 
      induced by Hcy.
FAU - Wang, Sen
AU  - Wang S
AD  - Key Laboratory for Medical Molecular Diagnostics of Guangdong Province, Guangdong 
      Medical University, Dongguan, Guangdong 523808, P.R. China.
FAU - Huang, Zhen
AU  - Huang Z
AD  - Longgang Central Hospital, Shenzhen, Guangdong 518116, P.R. China.
FAU - Li, Wenrui
AU  - Li W
AD  - Eighth People's Hospital of Dongguan City, Dongguan, Guangdong 523321, P.R. 
      China.
FAU - He, Suhui
AU  - He S
AD  - Key Laboratory for Medical Molecular Diagnostics of Guangdong Province, Guangdong 
      Medical University, Dongguan, Guangdong 523808, P.R. China.
FAU - Wu, Hongmei
AU  - Wu H
AD  - Longgang Central Hospital, Shenzhen, Guangdong 518116, P.R. China.
FAU - Zhu, Jiandong
AU  - Zhu J
AD  - Key Laboratory for Medical Molecular Diagnostics of Guangdong Province, Guangdong 
      Medical University, Dongguan, Guangdong 523808, P.R. China.
FAU - Li, Rumeng
AU  - Li R
AD  - Key Laboratory for Medical Molecular Diagnostics of Guangdong Province, Guangdong 
      Medical University, Dongguan, Guangdong 523808, P.R. China.
FAU - Liang, Zhuangyan
AU  - Liang Z
AD  - Key Laboratory for Medical Molecular Diagnostics of Guangdong Province, Guangdong 
      Medical University, Dongguan, Guangdong 523808, P.R. China.
FAU - Chen, Zhangquan
AU  - Chen Z
AD  - Key Laboratory for Medical Molecular Diagnostics of Guangdong Province, Guangdong 
      Medical University, Dongguan, Guangdong 523808, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20191106
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (IL1B protein, human)
RN  - 0 (IL37 protein, human)
RN  - 0 (IL6 protein, human)
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Interleukin-6)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0LVT1QZ0BA (Homocysteine)
RN  - EC 4.2.1.- (Hydro-Lyases)
RN  - EC 4.2.1.54 (lactate dehydratase)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Female
MH  - Gene Expression Regulation/drug effects
MH  - Homocysteine/*metabolism/pharmacology/toxicity
MH  - Humans
MH  - Hydro-Lyases/blood
MH  - Hyperhomocysteinemia/*blood/chemically induced/complications/genetics
MH  - Inflammation/*blood/chemically induced/complications/genetics
MH  - Interleukin-1/blood/*genetics
MH  - Interleukin-1beta/blood
MH  - Interleukin-6/blood
MH  - Male
MH  - Middle Aged
MH  - RNA, Messenger/blood
MH  - Tumor Necrosis Factor-alpha/blood/genetics
OTO - NOTNLM
OT  - il-37
OT  - expression
OT  - hHcy
OT  - inflammation
EDAT- 2019/11/21 06:00
MHDA- 2020/10/27 06:00
CRDT- 2019/11/21 06:00
PHST- 2019/03/31 00:00 [received]
PHST- 2019/09/04 00:00 [accepted]
PHST- 2019/11/21 06:00 [pubmed]
PHST- 2020/10/27 06:00 [medline]
PHST- 2019/11/21 06:00 [entrez]
AID - 10.3892/mmr.2019.10804 [doi]
PST - ppublish
SO  - Mol Med Rep. 2020 Jan;21(1):371-378. doi: 10.3892/mmr.2019.10804. Epub 2019 Nov 
      6.