PMID- 31746423
OWN - NLM
STAT- MEDLINE
DCOM- 20200603
LR  - 20211008
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Print)
IS  - 1791-2997 (Linking)
VI  - 21
IP  - 1
DP  - 2020 Jan
TI  - Endoplasmic reticulum stress regulates epithelial‑mesenchymal transition in human 
      lens epithelial cells.
PG  - 173-180
LID - 10.3892/mmr.2019.10814 [doi]
AB  - Epithelial‑to‑mesenchymal transition (EMT) of human lens epithelial cells (HLECs) 
      serve an important role in cataract formation. The endoplasmic reticulum stress 
      response (ER stress) has been demonstrated to regulate EMT in a number of 
      tissues. The aim of the present study was to demonstrate the role of ER stress on 
      EMT in HLECs. HLECs were treated with tunicamycin (TM) or thapsigargin (TG) to 
      disturb ER homeostasis, and 4‑phenylbutyric acid (PBA) or sodium 
      tauroursodeoxycholate (TUDCA) to restore ER homeostasis. Cell morphology was 
      evaluated after 24 h. The long axis and aspect ratio of the cells were analyzed 
      using ImageJ software. The results demonstrated that HLECs adopted an elongated 
      morphology following treatment with TG, and the cellular aspect ratio increased. 
      However, this morphological change was not observed following combination 
      treatment with TG and PBA. Western blot analysis and immunofluorescence staining 
      were used to measure the protein expression levels. A wound‑healing assay was 
      performed to evaluate cell migration. Treatment with TM or TG increased the 
      expression of the ER stress markers glucose‑regulated protein 78, phosphorylated 
      eukaryotic initiation factor 2alpha, activating transcription factor (ATF)6, ATF4 and 
      inositol‑requiring protein 1alpha and the EMT markers fibronectin, vimentin, alpha‑smooth 
      muscle actin and neural cadherin. Furthermore, treatment with TM or TG decreased 
      the expression of the epithelial cell marker epithelial cadherin and enhanced 
      cell migration, which effects were inhibited following treatment with PBA or 
      TUDCA. These results indicates that enhanced ER stress induced EMT and 
      subsequently increased cell migration in HLECs in vitro.
FAU - Zhou, Sheng
AU  - Zhou S
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat‑sen 
      University, Guangzhou, Guangdong 510060, P.R. China.
FAU - Yang, Jing
AU  - Yang J
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat‑sen 
      University, Guangzhou, Guangdong 510060, P.R. China.
FAU - Wang, Mingwei
AU  - Wang M
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat‑sen 
      University, Guangzhou, Guangdong 510060, P.R. China.
FAU - Zheng, Danying
AU  - Zheng D
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat‑sen 
      University, Guangzhou, Guangdong 510060, P.R. China.
FAU - Liu, Yizhi
AU  - Liu Y
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat‑sen 
      University, Guangzhou, Guangdong 510060, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20191112
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Eye Proteins)
RN  - 0 (Phenylbutyrates)
RN  - 11089-65-9 (Tunicamycin)
RN  - 516-35-8 (Taurochenodeoxycholic Acid)
RN  - 60EUX8MN5X (ursodoxicoltaurine)
RN  - 67526-95-8 (Thapsigargin)
RN  - 7WY7YBI87E (4-phenylbutyric acid)
SB  - IM
MH  - Cataract/metabolism/pathology
MH  - Cell Line
MH  - *Endoplasmic Reticulum Stress
MH  - Epithelial Cells/*metabolism/pathology
MH  - *Epithelial-Mesenchymal Transition
MH  - Eye Proteins/metabolism
MH  - Humans
MH  - Lens, Crystalline/*metabolism/pathology
MH  - Phenylbutyrates/pharmacology
MH  - Taurochenodeoxycholic Acid/pharmacology
MH  - Thapsigargin/pharmacology
MH  - Tunicamycin/pharmacology
PMC - PMC6896292
OTO - NOTNLM
OT  - human lens epithelial cells
OT  - endoplasmic reticulum stress response
OT  - epithelial-to-mesenchymal transition
OT  - unfolded protein response
EDAT- 2019/11/21 06:00
MHDA- 2020/06/04 06:00
PMCR- 2019/11/12
CRDT- 2019/11/21 06:00
PHST- 2019/05/08 00:00 [received]
PHST- 2019/10/24 00:00 [accepted]
PHST- 2019/11/21 06:00 [pubmed]
PHST- 2020/06/04 06:00 [medline]
PHST- 2019/11/21 06:00 [entrez]
PHST- 2019/11/12 00:00 [pmc-release]
AID - mmr-21-01-0173 [pii]
AID - 10.3892/mmr.2019.10814 [doi]
PST - ppublish
SO  - Mol Med Rep. 2020 Jan;21(1):173-180. doi: 10.3892/mmr.2019.10814. Epub 2019 Nov 
      12.