PMID- 31749479 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20231019 IS - 0022-1155 (Print) IS - 0975-8402 (Electronic) IS - 0022-1155 (Linking) VI - 56 IP - 12 DP - 2019 Dec TI - Salmonella prevalence and serovar distribution in healthy slaughter sheep and cattle determined by ISO 6579 and VIDAS UP Salmonella methods. PG - 5317-5325 LID - 10.1007/s13197-019-04002-2 [doi] AB - Prevalence of Salmonella in slaughter sheep and cattle was determined by International Organization for Standardization Method 6579 (ISO) and Vitek Immunodiagnostic Assay System UP Salmonella Phage Technology (VIDAS UP Salmonella SPT-VIDAS UP). A total of 400 healthy slaughter sheep (n = 200) and cattle (n = 200) carcass (C), fecal content (FC), mesenteric lymph node (MLN), liver (L), kidney (K), spleen (S) and gall bladder (GB) were randomly sampled and analysed. ISO and VIDAS UP results indicated 13 (3.25%) and 17 (4.25%) of 400 animals carried Salmonella, respectively, regardless of sample type. There was no isolation from L, S, GB, while 2 C (0.5%), 6 FC (1.5%), 7 MLN (1.75%), 3 K (0.75%) were contaminated with Salmonella. S. Typhimurium (27.8%), S. Enteritidis (22.2%), S. Newport (22.2%) were the three dominant serovars, followed by S. Kentucky (11.1%), S. Umbilo (5.6%), S. Corvallis (5.6%), and S. Albany (5.6%). Overall prevalence in 2800 samples was 0.46% by ISO and 0.61% by VIDAS UP. High relative trueness (RT: 99.79%) of VIDAS UP with a substantial agreement to ISO (kappa value: 0.80) indicated its efficiency to accompany ISO to monitor Salmonella in slaughter animals. As the first report to evaluate ISO and VIDAS UP in detecting Salmonella from slaughter sheep and cattle, this current prevalence signifies a risk for public health in red-meat and related products in Turkey. CI - (c) Association of Food Scientists & Technologists (India) 2019. FAU - Cetin, Ece AU - Cetin E AUID- ORCID: 0000-0002-8783-5507 AD - 1Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Tekirdag Namik Kemal University, 59030 Tekirdag, Turkey. GRID: grid.412006.1. ISNI: 0000 0004 0369 8053 FAU - Temelli, Seran AU - Temelli S AUID- ORCID: 0000-0002-8869-4929 AD - 2Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Bursa Uludag University, 16059 Bursa, Turkey. GRID: grid.34538.39. ISNI: 0000 0001 2182 4517 FAU - Eyigor, Aysegul AU - Eyigor A AUID- ORCID: 0000-0002-2707-3117 AD - 2Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Bursa Uludag University, 16059 Bursa, Turkey. GRID: grid.34538.39. ISNI: 0000 0001 2182 4517 LA - eng PT - Journal Article DEP - 20190806 PL - India TA - J Food Sci Technol JT - Journal of food science and technology JID - 0056471 PMC - PMC6838269 OTO - NOTNLM OT - Cattle OT - ISO OT - Salmonella OT - Sheep OT - VIDAS UP EDAT- 2019/11/22 06:00 MHDA- 2019/11/22 06:01 PMCR- 2020/12/01 CRDT- 2019/11/22 06:00 PHST- 2019/07/15 00:00 [revised] PHST- 2019/08/01 00:00 [accepted] PHST- 2019/11/22 06:00 [entrez] PHST- 2019/11/22 06:00 [pubmed] PHST- 2019/11/22 06:01 [medline] PHST- 2020/12/01 00:00 [pmc-release] AID - 4002 [pii] AID - 10.1007/s13197-019-04002-2 [doi] PST - ppublish SO - J Food Sci Technol. 2019 Dec;56(12):5317-5325. doi: 10.1007/s13197-019-04002-2. Epub 2019 Aug 6.