PMID- 31750260
OWN - NLM
STAT- MEDLINE
DCOM- 20200714
LR  - 20200714
IS  - 2235-2988 (Electronic)
IS  - 2235-2988 (Linking)
VI  - 9
DP  - 2019
TI  - Exosome-Transmitted miR-25 Induced by H. pylori Promotes Vascular Endothelial 
      Cell Injury by Targeting KLF2.
PG  - 366
LID - 10.3389/fcimb.2019.00366 [doi]
LID - 366
AB  - Background: Increasing evidence has shown that Helicobacter pylori is associated 
      with coronary heart disease (CHD); however, the underlying mechanism remains 
      unclear. Methods: The expression of miR-25 and mRNAs was measured using qRT-PCR. 
      Protein levels were detected using western blotting and exosomes were assessed 
      with an electron microscope. The target gene of miR-25 was identified using the 
      luciferase report system. Results:H. pylori infection increased the expression of 
      miR-25 in gastric epithelial cells and was associated with increased levels of 
      exosome-transmitted miR-25 in human peripheral blood. Mechanistic investigation 
      showed the Kruppel-like factor 2 (KLF2) was a direct target of 
      exosome-transmitted miR-25 in vascular endothelial cells. In addition, the 
      miR-25/KLF2 axis regulated the NF-kappaB signaling pathway, resulting in increased 
      expression of interleukin 6 (IL6), monocyte chemoattractant protein-1 (MCP-1), 
      vascular cell adhesion molecule-1 (VCAM-1), and intercellular adhesion molecule-1 
      (ICAM-1). Conclusion: Our findings suggest that the miR-25/KLF2 axis may be a 
      potential therapeutic target for H. pylori-associated CHD. Furthermore, high 
      levels of exosome-transmitted miR-25 in peripheral blood may pose a potential 
      risk for CHD.
CI  - Copyright (c) 2019 Li, Liu, Dong, Zhang, Huang, Wang and Wang.
FAU - Li, Na
AU  - Li N
AD  - Department of Pediatric Cardiac Surgery, Institute of Cardiovascular Disease, The 
      960th Hospital of Chinese PLA, Jinan, China.
FAU - Liu, Shi-Feng
AU  - Liu SF
AD  - Department of Pediatric Cardiac Surgery, Institute of Cardiovascular Disease, The 
      960th Hospital of Chinese PLA, Jinan, China.
FAU - Dong, Kai
AU  - Dong K
AD  - Department of Pediatric Cardiac Surgery, Institute of Cardiovascular Disease, The 
      960th Hospital of Chinese PLA, Jinan, China.
FAU - Zhang, Gui-Chun
AU  - Zhang GC
AD  - Department of Pediatric Cardiac Surgery, Institute of Cardiovascular Disease, The 
      960th Hospital of Chinese PLA, Jinan, China.
FAU - Huang, Jing
AU  - Huang J
AD  - Department of Pediatric Cardiac Surgery, Institute of Cardiovascular Disease, The 
      960th Hospital of Chinese PLA, Jinan, China.
FAU - Wang, Zhi-Heng
AU  - Wang ZH
AD  - Department of Pediatric Cardiac Surgery, Institute of Cardiovascular Disease, The 
      960th Hospital of Chinese PLA, Jinan, China.
FAU - Wang, Tong-Jian
AU  - Wang TJ
AD  - Department of Pediatric Cardiac Surgery, Institute of Cardiovascular Disease, The 
      960th Hospital of Chinese PLA, Jinan, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191029
PL  - Switzerland
TA  - Front Cell Infect Microbiol
JT  - Frontiers in cellular and infection microbiology
JID - 101585359
RN  - 0 (Biomarkers)
RN  - 0 (Inflammation Mediators)
RN  - 0 (KLF2 protein, human)
RN  - 0 (Kruppel-Like Transcription Factors)
RN  - 0 (MIRN25 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (NF-kappa B)
SB  - IM
MH  - Biomarkers/metabolism
MH  - Endothelial Cells/*metabolism
MH  - Exosomes/*metabolism
MH  - *Gene Expression Regulation
MH  - Helicobacter Infections/genetics/metabolism/microbiology
MH  - Helicobacter pylori/physiology
MH  - Humans
MH  - Inflammation Mediators/metabolism
MH  - Kruppel-Like Transcription Factors/*genetics
MH  - MicroRNAs/*genetics
MH  - NF-kappa B/metabolism
MH  - *RNA Interference
MH  - Signal Transduction
PMC - PMC6842922
OTO - NOTNLM
OT  - Helicobacter pylori
OT  - KLF2
OT  - NF-kappaB
OT  - coronary heart disease
OT  - miR-25
EDAT- 2019/11/22 06:00
MHDA- 2020/07/15 06:00
PMCR- 2019/01/01
CRDT- 2019/11/22 06:00
PHST- 2019/06/27 00:00 [received]
PHST- 2019/10/08 00:00 [accepted]
PHST- 2019/11/22 06:00 [entrez]
PHST- 2019/11/22 06:00 [pubmed]
PHST- 2020/07/15 06:00 [medline]
PHST- 2019/01/01 00:00 [pmc-release]
AID - 10.3389/fcimb.2019.00366 [doi]
PST - epublish
SO  - Front Cell Infect Microbiol. 2019 Oct 29;9:366. doi: 10.3389/fcimb.2019.00366. 
      eCollection 2019.