PMID- 31753476
OWN - NLM
STAT- MEDLINE
DCOM- 20210204
LR  - 20210204
IS  - 1879-3231 (Electronic)
IS  - 0093-691X (Linking)
VI  - 148
DP  - 2020 May
TI  - Tamoxifen-induced alterations in the expression of selected matrix 
      metalloproteinases (MMP-2, -9, -10, and -13) and their tissue inhibitors (TIMP-2 
      and -3) in the chicken ovary.
PG  - 208-215
LID - S0093-691X(19)30508-4 [pii]
LID - 10.1016/j.theriogenology.2019.11.008 [doi]
AB  - Matrix metalloproteinases (MMPs) are a family of peptidases that disintegrate 
      extracellular matrix (ECM) molecules associated with tissue remodeling, including 
      reproductive tissues. Their actions are largely controlled by specific tissue 
      inhibitors of MMPs (TIMPs). The role and regulation of MMPs in the chicken ovary 
      is largely unknown. The aim of the present study was to examine the effect of 
      tamoxifen (TMX; estrogen receptor modulator) treatment on the expression of 
      selected members of the MMP system in the laying hen ovary. The activity of MMP-2 
      and -9 was also examined. Real-time polymerase chain reaction and western blot 
      analyses revealed changes in mRNA and/or protein expression of MMP-2, -9, 
      -10, -13, TIMP-2, and TIMP-3 in the following ovarian follicles after TMX 
      treatment: white (WF), yellowish (YF), small yellow (SYF), and the largest yellow 
      preovulatory (F3-F1). The response to TMX depended on the stage of follicle 
      development and the layer of follicular wall. Moreover, ovarian regression 
      following TMX treatment was accompanied by both an increase in total activity of 
      MMP-2 in the theca layer of F3-F2 and granulosa layer of F2, and a decrease in 
      total activity of MMP-2 in the WF, YF, and SYF, and MMP-9 in theca of F3-F1. In 
      conclusion, the TMX-induced changes in MMP-2, -9, -10, and -13, and TIMP-2 and -3 
      mRNA expression, as well as MMP-2 and -9 activity, were dependent on tissue and 
      the stage of follicular maturation. Our findings strongly suggests a role for 
      estrogen in regulating the transcription, translation, and/or posttranslational 
      activity of members of the MMP system. Further, these components may be involved 
      in the orchestration of ECM turnover and cellular functions during ovary 
      regression, which occur under conditions of reduced estrogenic activity.
CI  - Copyright (c) 2019 Elsevier Inc. All rights reserved.
FAU - Wolak, Dominika
AU  - Wolak D
AD  - Department of Animal Physiology and Endocrinology, University of Agriculture in 
      Krakow, al. Mickiewicza 24/28, 30-059, Krakow, Poland.
FAU - Hrabia, Anna
AU  - Hrabia A
AD  - Department of Animal Physiology and Endocrinology, University of Agriculture in 
      Krakow, al. Mickiewicza 24/28, 30-059, Krakow, Poland. Electronic address: 
      rzhrabia@cyf-kr.edu.pl.
LA  - eng
PT  - Journal Article
PT  - Randomized Controlled Trial, Veterinary
DEP - 20191109
PL  - United States
TA  - Theriogenology
JT  - Theriogenology
JID - 0421510
RN  - 0 (Estrogen Antagonists)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-3)
RN  - 094ZI81Y45 (Tamoxifen)
RN  - 127497-59-0 (Tissue Inhibitor of Metalloproteinase-2)
RN  - EC 3.4.24.- (Matrix Metalloproteinase 13)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
RN  - EC 3.4.24.22 (Matrix Metalloproteinase 10)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Animals
MH  - Chickens
MH  - Estrogen Antagonists/pharmacology
MH  - Female
MH  - Gene Expression Regulation/*drug effects
MH  - Matrix Metalloproteinase 10/genetics/metabolism
MH  - Matrix Metalloproteinase 13/genetics/metabolism
MH  - Matrix Metalloproteinase 2/genetics/metabolism
MH  - Matrix Metalloproteinase 9/genetics/metabolism
MH  - Matrix Metalloproteinases/genetics/*metabolism
MH  - Organ Size
MH  - Ovary/anatomy & histology/*drug effects
MH  - Tamoxifen/*pharmacology
MH  - Tissue Inhibitor of Metalloproteinase-2/genetics/*metabolism
MH  - Tissue Inhibitor of Metalloproteinase-3/genetics/*metabolism
OTO - NOTNLM
OT  - Chicken
OT  - MMPs
OT  - Ovary
OT  - TIMPs
OT  - Tamoxifen
EDAT- 2019/11/23 06:00
MHDA- 2021/02/05 06:00
CRDT- 2019/11/23 06:00
PHST- 2019/09/17 00:00 [received]
PHST- 2019/11/04 00:00 [revised]
PHST- 2019/11/09 00:00 [accepted]
PHST- 2019/11/23 06:00 [pubmed]
PHST- 2021/02/05 06:00 [medline]
PHST- 2019/11/23 06:00 [entrez]
AID - S0093-691X(19)30508-4 [pii]
AID - 10.1016/j.theriogenology.2019.11.008 [doi]
PST - ppublish
SO  - Theriogenology. 2020 May;148:208-215. doi: 10.1016/j.theriogenology.2019.11.008. 
      Epub 2019 Nov 9.