PMID- 31755557
OWN - NLM
STAT- MEDLINE
DCOM- 20200427
LR  - 20200427
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Print)
IS  - 0019-2805 (Linking)
VI  - 159
IP  - 3
DP  - 2020 Mar
TI  - TRIM21 controls Toll-like receptor 2 responses in bone-marrow-derived 
      macrophages.
PG  - 335-343
LID - 10.1111/imm.13157 [doi]
AB  - TRIM21 is an interferon-stimulated E3 ligase that controls the activity of 
      pattern-recognition signaling via ubiquitination of interferon regulatory factors 
      and DDX41. Previous studies on the role of TRIM21 in innate immune responses have 
      yielded contradictory results, suggesting that the role of TRIM21 is cell 
      specific. Here, we report that bone-marrow-derived macrophages (BMDMs) generated 
      from Trim21(-/-) mice have reduced expression of mature macrophage markers. 
      Reflecting their reduced differentiation in response to macrophage 
      colony-stimulating factor (M-CSF), Trim21(-/-) BMDMs had decreased expression of 
      M-CSF signature genes. Although Trim21(-/-) BMDMs responded normally to Toll-like 
      receptor 9 (TLR9) activation, they produced lower levels of pro-inflammatory 
      cytokines in response to the TLR2 agonist PAM3CSK4. In line with this, the 
      response to infection with the Bacillus Calmette-Guerin strain of Mycobacterium 
      bovis was also diminished in Trim21(-/-) BMDMs. Our results indicate that TRIM21 
      controls responses to TLR2 agonists.
CI  - (c) 2019 The Authors. Immunology published by John Wiley & Sons Ltd.
FAU - Sjostrand, Maria
AU  - Sjostrand M
AD  - Unit of Rheumatology, Center for Molecular Medicine L8:03, Department of 
      Medicine, Karolinska Institutet, Karolinska University Hospital, Stockholm, 
      Sweden.
FAU - Carow, Berit
AU  - Carow B
AD  - Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, 
      Stockholm, Sweden.
FAU - Nyberg, William A
AU  - Nyberg WA
AD  - Unit of Rheumatology, Center for Molecular Medicine L8:03, Department of 
      Medicine, Karolinska Institutet, Karolinska University Hospital, Stockholm, 
      Sweden.
FAU - Covacu, Ruxandra
AU  - Covacu R
AD  - Unit of Neuroimmunology, Department of Clinical Neuroscience, Center for 
      Molecular Medicine, Karolinska Institutet, Karolinska University Hospital, 
      Stockholm, Sweden.
FAU - Rottenberg, Martin E
AU  - Rottenberg ME
AD  - Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, 
      Stockholm, Sweden.
FAU - Espinosa, Alexander
AU  - Espinosa A
AUID- ORCID: 0000-0003-1107-776X
AD  - Unit of Rheumatology, Center for Molecular Medicine L8:03, Department of 
      Medicine, Karolinska Institutet, Karolinska University Hospital, Stockholm, 
      Sweden.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191212
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Cytokines)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Lipopeptides)
RN  - 0 (Pam(3)CSK(4) peptide)
RN  - 0 (Ribonucleoproteins)
RN  - 0 (SS-A antigen)
RN  - 0 (Tlr2 protein, mouse)
RN  - 0 (Toll-Like Receptor 2)
RN  - 81627-83-0 (Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Cytokines/*metabolism
MH  - Host-Pathogen Interactions
MH  - Inflammation Mediators/*metabolism
MH  - Lipopeptides/pharmacology
MH  - Macrophage Colony-Stimulating Factor/pharmacology
MH  - Macrophages/drug effects/immunology/*metabolism/microbiology
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Mycobacterium bovis/immunology/pathogenicity
MH  - Phenotype
MH  - Ribonucleoproteins/deficiency/genetics/*metabolism
MH  - Signal Transduction
MH  - Toll-Like Receptor 2/agonists/genetics/*metabolism
PMC - PMC7011629
OTO - NOTNLM
OT  - TRIM21
OT  - Toll-like receptor 2
OT  - macrophage
EDAT- 2019/11/23 06:00
MHDA- 2020/04/28 06:00
PMCR- 2019/12/12
CRDT- 2019/11/23 06:00
PHST- 2019/02/13 00:00 [received]
PHST- 2019/11/14 00:00 [revised]
PHST- 2019/11/18 00:00 [accepted]
PHST- 2019/11/23 06:00 [pubmed]
PHST- 2020/04/28 06:00 [medline]
PHST- 2019/11/23 06:00 [entrez]
PHST- 2019/12/12 00:00 [pmc-release]
AID - IMM13157 [pii]
AID - 10.1111/imm.13157 [doi]
PST - ppublish
SO  - Immunology. 2020 Mar;159(3):335-343. doi: 10.1111/imm.13157. Epub 2019 Dec 12.