PMID- 31758371
OWN - NLM
STAT- MEDLINE
DCOM- 20201023
LR  - 20201023
IS  - 1573-5168 (Electronic)
IS  - 0920-1742 (Linking)
VI  - 46
IP  - 1
DP  - 2020 Feb
TI  - Grass carp (Ctenopharyngodon idella) NRF2 alleviates the oxidative stress and 
      enhances cell viability through upregulating the expression of HO-1.
PG  - 417-428
LID - 10.1007/s10695-019-00729-z [doi]
AB  - As a member of the Cap 'n' Collar (CNC) family, NRF2 contains a basic leucine 
      zipper (bZip) and can regulate the downstream target gene heme oxygenase 1 (HO-1) 
      in response to oxidative stress. In the present study, a grass carp 
      (Ctenopharyngodon idella) NRF2 ORF was cloned and identified. The largest ORF 
      (1782 bp) encodes a polypeptide of 593 amino acids. The deduced amino acid 
      sequence of grass carp NRF2 (CiNRF2) contains a well-conserved DNA-binding domain 
      (BRLZ domain). Phylogenetic tree analysis revealed that CiNRF2 has a closer 
      evolutionary relationship with other fish counterparts. After CIK (C. idellus 
      kidney) cells were persistently stimulated with tunicamycin (TM), CiNRF2 was 
      significantly upregulated from 12 to 36 h. Then, the expression was dropped at 48 
      h post-infection. Additionally, when TM or TG (thapsigargin) stimulated CIK 
      cells, overexpression of CiNRF2 in cells downregulated the expression of Bip 
      mRNA, a marker protein of oxidative stress, suggesting that fish NRF2 can 
      alleviate the oxidative stress level induced by TM or TG. To study the protective 
      mechanism of fish NRF2, the DNA sequences of CiNRF2 and CiATF4 (grass carp ATF4) 
      were separately sub-cloned into the expression vectors pEGFP and pCMV-Flag for 
      co-immunoprecipitation and GST pull-down assays. These assays showed that CiNRF2 
      can combine with CiATF4 through its Neh1 domain. Meanwhile, we cloned grass carp 
      HO-1 promoter sequence and constructed the recombinant plasmid of pGL3-HO-1. Soon 
      afterwards, pGL3-HO-1 was co-transfected into grass carp ovary (CO) cells with 
      pcDNA3.1-CiNRF2 or pcDNA3.1-CiATF4, respectively. The results showed that the 
      luciferase activity of pGL3-HO-1 in the overexpressed CiNRF2 plus CiATF4 cells 
      was significantly increased, along with the increase of cell viability (~ 133%). 
      However, when HO-1 was knocked down in cells, CiNRF2 was unable to perform its 
      function. These results demonstrated that CiNRF2 was effective in protecting 
      grass carp against the oxidative stress induced by TM and increasing cell 
      viability by upregulating HO-1 expression.
FAU - Chen, Xin
AU  - Chen X
AD  - Department of Bioscience, College of Life Science, Nanchang University, Nanchang, 
      330031, China.
AD  - Poyang Lake Key Laboratory of Environment and Resource Utilization, Ministry of 
      Education, Nanchang University, Nanchang, China.
FAU - Han, Kun
AU  - Han K
AD  - Department of Bioscience, College of Life Science, Nanchang University, Nanchang, 
      330031, China.
AD  - Poyang Lake Key Laboratory of Environment and Resource Utilization, Ministry of 
      Education, Nanchang University, Nanchang, China.
FAU - Zhang, Tao
AU  - Zhang T
AD  - Department of Bioscience, College of Life Science, Nanchang University, Nanchang, 
      330031, China.
AD  - Poyang Lake Key Laboratory of Environment and Resource Utilization, Ministry of 
      Education, Nanchang University, Nanchang, China.
FAU - Qi, Guoqin
AU  - Qi G
AD  - Department of Bioscience, College of Life Science, Nanchang University, Nanchang, 
      330031, China.
AD  - Poyang Lake Key Laboratory of Environment and Resource Utilization, Ministry of 
      Education, Nanchang University, Nanchang, China.
FAU - Jiang, Zeyin
AU  - Jiang Z
AD  - Department of Bioscience, College of Life Science, Nanchang University, Nanchang, 
      330031, China.
AD  - Poyang Lake Key Laboratory of Environment and Resource Utilization, Ministry of 
      Education, Nanchang University, Nanchang, China.
FAU - Hu, Chengyu
AU  - Hu C
AD  - Department of Bioscience, College of Life Science, Nanchang University, Nanchang, 
      330031, China. hucy2008@163.com.
AD  - Poyang Lake Key Laboratory of Environment and Resource Utilization, Ministry of 
      Education, Nanchang University, Nanchang, China. hucy2008@163.com.
LA  - eng
GR  - 20171ACB20004/Natural Science Foundation of Jiangxi Province/
PT  - Journal Article
DEP - 20191122
PL  - Netherlands
TA  - Fish Physiol Biochem
JT  - Fish physiology and biochemistry
JID - 100955049
RN  - 0 (DNA, Complementary)
RN  - 0 (Fish Proteins)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (RNA, Messenger)
RN  - EC 1.14.14.18 (Heme Oxygenase-1)
SB  - IM
MH  - Animals
MH  - Carps/*physiology
MH  - DNA, Complementary
MH  - Fish Proteins/*genetics/metabolism
MH  - Gene Expression Regulation
MH  - Heme Oxygenase-1/*genetics/metabolism
MH  - NF-E2-Related Factor 2/*genetics/metabolism
MH  - Oxidative Stress/*physiology
MH  - RNA, Messenger
OTO - NOTNLM
OT  - Fish
OT  - HO-1
OT  - NRF2 and ATF4
OT  - Oxidative stress
OT  - PERK
EDAT- 2019/11/24 06:00
MHDA- 2020/10/24 06:00
CRDT- 2019/11/24 06:00
PHST- 2018/12/05 00:00 [received]
PHST- 2019/11/01 00:00 [accepted]
PHST- 2019/11/24 06:00 [pubmed]
PHST- 2020/10/24 06:00 [medline]
PHST- 2019/11/24 06:00 [entrez]
AID - 10.1007/s10695-019-00729-z [pii]
AID - 10.1007/s10695-019-00729-z [doi]
PST - ppublish
SO  - Fish Physiol Biochem. 2020 Feb;46(1):417-428. doi: 10.1007/s10695-019-00729-z. 
      Epub 2019 Nov 22.