PMID- 31759680
OWN - NLM
STAT- MEDLINE
DCOM- 20210212
LR  - 20210212
IS  - 1532-2785 (Electronic)
IS  - 0143-4179 (Linking)
VI  - 81
DP  - 2020 Jun
TI  - Neuropeptides are associated with pain threshold and bone microstructure in 
      ovariectomized rats.
PG  - 101995
LID - S0143-4179(19)30176-3 [pii]
LID - 10.1016/j.npep.2019.101995 [doi]
AB  - OBJECTIVES: Postmenopausal osteoporosis (PMO) is a metabolic skeletal disorder 
      with impaired bone density and bone quality in postmenopausal women. The aim of 
      the present study was to investigate the correlation between neuropeptides, bone 
      microstructure and pain threshold in ovariectomized (OVX) rats. METHODS: Female 
      rats were randomly divided into the ovariectomized (OVX) group and the sham 
      surgery (SHAM) group. Bone microstructure and immunocytochemistry for substance P 
      (SP), calcitonin gene-related peptide (CGRP), vasoactive intestinal peptide (VIP) 
      and neuropeptide Y (NPY) in tibial and DRG were performed. Pain threshold was 
      assessed at post-operative 11 weeks. Pearson correlation coefficients were 
      calculated between neuropeptides, bone microstructure and pain threshold. 
      RESULTS: Significant decreases in bone volume fraction (BV/TV) and trabecular 
      number (Tb. N) but significant increases in trabecular spacing (Tb.Sp) were 
      showed in OVX group. Mechanical pain threshold (MPT) in OVX group was 
      significantly decreased. The MOD values for SP, CGRP and VIP of tibial in OVX 
      group were significantly lower, whereas NPY, NPY1R and NPY2R were significantly 
      higher. And SP, CGRP, VIP, NPY and NPY2R of DRG were significantly increased in 
      OVX group, while NPY1R was significantly decreased. Correlation analysis showed 
      that NPY, Y1R and Y2R in bone were negatively correlated with BV/TV. MPT was 
      negatively correlated with NPY and Y2R in DRG, and positively correlated with Y1R 
      in DRG. CONCLUSIONS: Our results suggested that SP, CGRP, VIP and NPY were 
      involved in the osteoporotic bone microstructure and mechanical hypersensitivity 
      in OVX rats, indicating the potential to utilize neuropeptides as novel 
      therapeutic targets for PMO.
CI  - Copyright (c) 2019 Elsevier Ltd. All rights reserved.
FAU - Xie, Weixin
AU  - Xie W
AD  - Department of Orthopaedic Surgery, Renji Hospital, School of Medicine, Shanghai 
      Jiaotong University, Shanghai 200127, China.
FAU - Li, Fan
AU  - Li F
AD  - Department of Orthopaedic Surgery, Renji Hospital, School of Medicine, Shanghai 
      Jiaotong University, Shanghai 200127, China.
FAU - Han, Yi
AU  - Han Y
AD  - Department of Orthopaedic Surgery, Renji Hospital, School of Medicine, Shanghai 
      Jiaotong University, Shanghai 200127, China.
FAU - Li, Zhanchun
AU  - Li Z
AD  - Department of Orthopaedic Surgery, Renji Hospital, School of Medicine, Shanghai 
      Jiaotong University, Shanghai 200127, China. Electronic address: 
      lzctgzyyx@163.com.
FAU - Xiao, Jie
AU  - Xiao J
AD  - Department of Anesthesiology, Renji Hospital, School of Medicine, Shanghai 
      Jiaotong University, Shanghai 200127, China. Electronic address: 
      applexiaomz@163.com.
LA  - eng
PT  - Journal Article
DEP - 20191113
PL  - Netherlands
TA  - Neuropeptides
JT  - Neuropeptides
JID - 8103156
RN  - 0 (Neuropeptides)
SB  - IM
MH  - Animals
MH  - Bone Density
MH  - Bone and Bones/*metabolism/*pathology
MH  - Disease Models, Animal
MH  - Ganglia, Spinal/metabolism
MH  - Humans
MH  - Hyperalgesia/metabolism
MH  - Neuropeptides/*metabolism
MH  - Osteoporosis, Postmenopausal/*metabolism/pathology/psychology
MH  - Ovariectomy
MH  - Pain Threshold/*physiology
MH  - Rats, Sprague-Dawley
OTO - NOTNLM
OT  - Bone microstructure
OT  - Neuropeptide
OT  - Osteoporosis
OT  - Pain threshold
COIS- Declaration of Competing Interest The authors have no conflict of interest to 
      disclose.
EDAT- 2019/11/25 06:00
MHDA- 2021/02/13 06:00
CRDT- 2019/11/25 06:00
PHST- 2019/09/26 00:00 [received]
PHST- 2019/11/04 00:00 [revised]
PHST- 2019/11/13 00:00 [accepted]
PHST- 2019/11/25 06:00 [pubmed]
PHST- 2021/02/13 06:00 [medline]
PHST- 2019/11/25 06:00 [entrez]
AID - S0143-4179(19)30176-3 [pii]
AID - 10.1016/j.npep.2019.101995 [doi]
PST - ppublish
SO  - Neuropeptides. 2020 Jun;81:101995. doi: 10.1016/j.npep.2019.101995. Epub 2019 Nov 
      13.