PMID- 31760580 OWN - NLM STAT- MEDLINE DCOM- 20201119 LR - 20201119 IS - 1559-1166 (Electronic) IS - 0895-8696 (Linking) VI - 70 IP - 2 DP - 2020 Feb TI - Expression Analysis of BDNF, BACE1, and Their Natural Occurring Antisenses in Autistic Patients. PG - 194-200 LID - 10.1007/s12031-019-01432-7 [doi] AB - Autism spectrum disorder (ASD) as a multifaceted neurological syndrome affects many aspects of neuropsychologic functions. Dysregulated expressions of several genes have been documented in ASD patients. The current project aimed at comparison of transcript levels of brain derived neurotrophic factor (BDNF), beta-site amyloid precursor protein cleaving enzyme 1 (BACE1), and their natural occurring antisenses in the peripheral blood of ASD individuals (n = 50, male/female = 38/12, age (mean +/- standard deviation (SD)): 6 +/- 1.4, age range: 3-8) and matched healthy persons (n = 50, male/female = 37/13, age (mean +/- SD): 6 +/- 1.74, age range: 3-8). We demonstrated remarkable higher levels of these genes in ASD patients. BACE1 transcript levels were correlated with transcript levels of BACE1-AS in all study participants. However, BACE1 transcript levels were not correlated with participants' age. BACE1-AS and BDNF transcript levels were correlated with age in female participants. Significant correlations were detected between transcript levels of BDNF and those of other genes in all study groups. The current results render further indications for contribution of BDNF, BACE1, and their antisenses in the course of ASD and suggested expression levels of these transcripts as putative markers for this neurobehavioral disorder. Such results might be applied in clinical setting for diagnosis of complicated ASD cases. FAU - Ghafouri-Fard, Soudeh AU - Ghafouri-Fard S AD - Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran. FAU - Namvar, Amir AU - Namvar A AD - Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran. FAU - Arsang-Jang, Shahram AU - Arsang-Jang S AD - Clinical Research Development Center (CRDU), Qom University of Medical Sciences, Qom, Iran. FAU - Komaki, Alireza AU - Komaki A AD - Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, Iran. FAU - Taheri, Mohammad AU - Taheri M AUID- ORCID: 0000-0001-8381-0591 AD - Urogenital Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. mohammad_823@yahoo.com. LA - eng PT - Journal Article DEP - 20191123 PL - United States TA - J Mol Neurosci JT - Journal of molecular neuroscience : MN JID - 9002991 RN - 0 (Biomarkers) RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (RNA, Antisense) RN - 0 (RNA, Messenger) RN - EC 3.4.- (Amyloid Precursor Protein Secretases) RN - EC 3.4.23.- (Aspartic Acid Endopeptidases) RN - EC 3.4.23.46 (BACE1 protein, human) SB - IM MH - Amyloid Precursor Protein Secretases/*genetics/metabolism MH - Aspartic Acid Endopeptidases/*genetics/metabolism MH - Autistic Disorder/blood/*genetics MH - Biomarkers/blood MH - Blood Cells/metabolism MH - Brain-Derived Neurotrophic Factor/*genetics/metabolism MH - Child MH - Female MH - Humans MH - Male MH - RNA, Antisense/blood MH - RNA, Messenger/blood OTO - NOTNLM OT - Autism OT - BACE1 OT - BACE1-AS OT - BDNF OT - BDNF-AS EDAT- 2019/11/25 06:00 MHDA- 2020/11/20 06:00 CRDT- 2019/11/25 06:00 PHST- 2019/07/23 00:00 [received] PHST- 2019/11/03 00:00 [accepted] PHST- 2019/11/25 06:00 [pubmed] PHST- 2020/11/20 06:00 [medline] PHST- 2019/11/25 06:00 [entrez] AID - 10.1007/s12031-019-01432-7 [pii] AID - 10.1007/s12031-019-01432-7 [doi] PST - ppublish SO - J Mol Neurosci. 2020 Feb;70(2):194-200. doi: 10.1007/s12031-019-01432-7. Epub 2019 Nov 23.