PMID- 31761720
OWN - NLM
STAT- MEDLINE
DCOM- 20210407
LR  - 20210407
IS  - 1472-6491 (Electronic)
IS  - 1472-6483 (Linking)
VI  - 40
IP  - 1
DP  - 2020 Jan
TI  - Ovarian stimulation does not influence the uterine immune environment in healthy 
      infertile women.
PG  - 113-123
LID - S1472-6483(19)30698-4 [pii]
LID - 10.1016/j.rbmo.2019.08.004 [doi]
AB  - RESEARCH QUESTION: There is some controversy regarding the impact of ovarian 
      stimulation on immune cells in women undergoing IVF. The study's aim was to 
      determine whether ovarian stimulation affected immune uterine cells in healthy 
      women undergoing IVF. DESIGN: This prospective cohort study included 28 patients 
      undergoing IVF and 47 healthy oocyte donors. Endometrial biopsies were taken in a 
      natural cycle and after ovarian stimulation. All participants had a normal 
      karyotype, pelvic ultrasound and cervical cytology results and 
      thyroid-stimulating hormone concentration, as well as normal glucose and insulin 
      concentrations and inherited and acquired thrombophilia test results. Screening 
      tests including human papillomavirus were normal. Immune cells were analysed 
      using three techniques: fluorescence-activated cell sorting, immunohistochemistry 
      and gene expression. A human leukocyte antigen (HLA)-C tetramer was used as an 
      'artificial embryo'. The expression of genes including those for tumour necrosis 
      factor (TNF)-alpha and interleukin-10 (IL-10) was analysed. RESULTS: A comparison was 
      made of the percentage and gene expression of CD56(bright)CD16(-) uterine natural 
      killer (uNK), CD56(dim)CD16(+) natural killer cells, CD56(-)CD16(+) natural 
      killer cells and TregCD25(+)CD4(+)FoxP3(+) cells, uNK binding to the HLA-C 
      tetramer, and TNF-alpha and IL-10 expression. No between- or within-group differences 
      were observed in natural versus ovarian stimulation cycles. CONCLUSIONS: Ovarian 
      stimulation does not affect the uterine immune cell population or HLA-C binding 
      in healthy women undergoing ovarian stimulation. Further studies are underway to 
      find out if different responses might be seen in women with previous autoimmune 
      disorders.
CI  - Copyright (c) 2019 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All 
      rights reserved.
FAU - Alecsandru, D
AU  - Alecsandru D
AD  - Department of Immunology, IVI RMA, Madrid, Spain; Rey Juan Carlos University, 
      Madrid, Spain; Health Research Institute La Fe, Valencia, Spain. Electronic 
      address: diana.alecsandru@ivirma.com.
FAU - Pacheco, A
AU  - Pacheco A
AD  - Health Research Institute La Fe, Valencia, Spain; Department of Andrology, IVI 
      RMA, Madrid, Spain; Alfonso X 'El Sabio' University, Madrid, Spain.
FAU - Guerrero-Mayo, A
AU  - Guerrero-Mayo A
AD  - Universidad de Valencia, IVI Learning Center.
FAU - Fabris, A
AU  - Fabris A
AD  - Department of Reproductive Endocrinology and Infertility, IVI RMA, Madrid, Spain.
FAU - Aparicio, P
AU  - Aparicio P
AD  - Department of Immunology, IVI RMA, Madrid, Spain.
FAU - Barrio, A
AU  - Barrio A
AD  - Department of Reproductive Endocrinology and Infertility, IVI RMA, Madrid, Spain.
FAU - Pellicer, A
AU  - Pellicer A
AD  - Health Research Institute La Fe, Valencia, Spain; Universidad de Valencia, IVI 
      Learning Center; Department of Reproductive Endocrinology and Infertility, IVI 
      RMA, Roma, Italy.
FAU - Garcia-Velasco, Juan A
AU  - Garcia-Velasco JA
AD  - Rey Juan Carlos University, Madrid, Spain; Health Research Institute La Fe, 
      Valencia, Spain; Universidad de Valencia, IVI Learning Center; Department of 
      Reproductive Endocrinology and Infertility, IVI RMA, Madrid, Spain.
LA  - eng
PT  - Journal Article
DEP - 20190822
PL  - Netherlands
TA  - Reprod Biomed Online
JT  - Reproductive biomedicine online
JID - 101122473
SB  - IM
MH  - Adult
MH  - Embryo Implantation/immunology
MH  - Female
MH  - Humans
MH  - Infertility, Female/*immunology
MH  - Killer Cells, Natural/*immunology
MH  - Menstrual Cycle/immunology
MH  - *Ovulation Induction
MH  - Prospective Studies
MH  - Uterus/*immunology
OTO - NOTNLM
OT  - Endometrial biopsies
OT  - Immune cell populations
OT  - Natural cycles
OT  - Ovarian stimulation
OT  - Peripheral blood natural killer cells
OT  - Uterine natural killer cells
EDAT- 2019/11/26 06:00
MHDA- 2021/04/10 06:00
CRDT- 2019/11/26 06:00
PHST- 2019/01/16 00:00 [received]
PHST- 2019/07/15 00:00 [revised]
PHST- 2019/08/14 00:00 [accepted]
PHST- 2019/11/26 06:00 [pubmed]
PHST- 2021/04/10 06:00 [medline]
PHST- 2019/11/26 06:00 [entrez]
AID - S1472-6483(19)30698-4 [pii]
AID - 10.1016/j.rbmo.2019.08.004 [doi]
PST - ppublish
SO  - Reprod Biomed Online. 2020 Jan;40(1):113-123. doi: 10.1016/j.rbmo.2019.08.004. 
      Epub 2019 Aug 22.