PMID- 31765944 OWN - NLM STAT- MEDLINE DCOM- 20200220 LR - 20200220 IS - 1879-1514 (Electronic) IS - 0166-445X (Linking) VI - 218 DP - 2020 Jan TI - MicroRNA-124 regulates lactate transportation in the muscle of largemouth bass (micropterus salmoides) under hypoxia by targeting MCT1. PG - 105359 LID - S0166-445X(19)30690-3 [pii] LID - 10.1016/j.aquatox.2019.105359 [doi] AB - Carbohydrate metabolism switches from aerobic to anaerobic (glycolysis) to supply energy in response to acute hypoxic stress. Acute hypoxic stress with dissolved oxygen (DO) levels of 1.2 +/- 0.1 mg/L for 24 h and 12 h re-oxygenation was used to investigate the response of the anaerobic glycolytic pathway in Micropterus salmoides muscle. The results showed that the glucose concentration was significantly lower in muscle, while the lactic acid and pyruvic acid concentrations tended to increase during hypoxic stress. No significant difference was observed in muscle glycogen, and ATP content fluctuated significantly. The activities of gluconeogenesis-related enzymes were slightly elevated, such as phosphoenolpyruvate carboxykinase (PEPCK). The activities of the glycolytic enzymes increased after the induction of hypoxia, such as hexokinase (HK), pyruvate kinase (PK), and lactate dehydrogenase (LDH). Curiously, phosphofructokinase (PFK) activity was significantly down-regulated within 4 h during hypoxia, although these effects were transient, and most indices returned to control levels after 12 h of re-oxygenation. Upregulated hif-1alpha, ampkalpha, hk, glut1, and ldh mRNA expression suggested that carbohydrate metabolism was reprogrammed under hypoxia. Lactate transport was regulated by miR-124-5p according to quantitative polymerase chain reaction and dual luciferase reporter assays. Our findings provide new insight into the molecular regulatory mechanism of hypoxia in Micropterus salmoides muscle. CI - Copyright (c) 2019. Published by Elsevier B.V. FAU - Zhao, L L AU - Zhao LL AD - College of Animal Science and Technology, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, China. Electronic address: zhaoliulan2007@163.com. FAU - Wu, H AU - Wu H AD - College of Animal Science and Technology, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, China; Hunan Fisheries Science Institute, Changsha, 410153, China. Electronic address: wh17380133463@163.com. FAU - Sun, J L AU - Sun JL AD - College of Animal Science and Technology, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, China. Electronic address: 1988sunjunlong@sina.com. FAU - Liao, L AU - Liao L AD - College of Animal Science and Technology, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, China. Electronic address: 1325079864@qq.com. FAU - Cui, C AU - Cui C AD - College of Animal Science and Technology, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, China. Electronic address: 1297316616@qq.com. FAU - Liu, Q AU - Liu Q AD - College of Animal Science and Technology, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, China. Electronic address: liuqiaoafnj@hotmail.com. FAU - Luo, J AU - Luo J AD - College of Animal Science and Technology, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, China. Electronic address: luojie93815@163.com. FAU - Tang, X H AU - Tang XH AD - College of Animal Science and Technology, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, China. Electronic address: 348404937@163.com. FAU - Luo, W AU - Luo W AD - College of Animal Science and Technology, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, China. Electronic address: lwdjn@live.cn. FAU - Ma, J D AU - Ma JD AD - College of Animal Science and Technology, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, China. Electronic address: jideng.ma@sicau.edu.cn. FAU - Ye, X AU - Ye X AD - Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, GuangZhou 510380, China. Electronic address: gzyexing@163.com. FAU - Li, S J AU - Li SJ AD - Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, GuangZhou 510380, China. Electronic address: ssjjli@163.com. FAU - Yang, S AU - Yang S AD - College of Animal Science and Technology, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, China. Electronic address: ysys210@hotmail.com. LA - eng PT - Journal Article DEP - 20191116 PL - Netherlands TA - Aquat Toxicol JT - Aquatic toxicology (Amsterdam, Netherlands) JID - 8500246 RN - 0 (MicroRNAs) RN - 0 (Monocarboxylic Acid Transporters) RN - 0 (Symporters) RN - 0 (monocarboxylate transport protein 1) RN - 33X04XA5AT (Lactic Acid) RN - S88TT14065 (Oxygen) SB - IM MH - Acclimatization/genetics/*physiology MH - Animals MH - Bass/genetics/*metabolism MH - Carbohydrate Metabolism/genetics/physiology MH - Gene Expression Regulation MH - Hypoxia/genetics/*metabolism MH - Lactic Acid/*metabolism MH - MicroRNAs/*genetics MH - Monocarboxylic Acid Transporters/*genetics MH - Muscles/enzymology/*metabolism MH - Oxygen/metabolism MH - Symporters/*genetics OTO - NOTNLM OT - Glucose metabolism OT - Hypoxic stress OT - Largemouth bass OT - MCT OT - microRNA EDAT- 2019/11/26 06:00 MHDA- 2020/02/23 06:00 CRDT- 2019/11/26 06:00 PHST- 2019/08/28 00:00 [received] PHST- 2019/11/11 00:00 [revised] PHST- 2019/11/14 00:00 [accepted] PHST- 2019/11/26 06:00 [pubmed] PHST- 2020/02/23 06:00 [medline] PHST- 2019/11/26 06:00 [entrez] AID - S0166-445X(19)30690-3 [pii] AID - 10.1016/j.aquatox.2019.105359 [doi] PST - ppublish SO - Aquat Toxicol. 2020 Jan;218:105359. doi: 10.1016/j.aquatox.2019.105359. Epub 2019 Nov 16.