PMID- 31766621
OWN - NLM
STAT- MEDLINE
DCOM- 20200406
LR  - 20200408
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 24
IP  - 23
DP  - 2019 Nov 22
TI  - Curdlan (Alcaligenes faecalis) (1-->3)-beta-d-Glucan Oligosaccharides Drive M1 
      Phenotype Polarization in Murine Bone Marrow-Derived Macrophages via Activation 
      of MAPKs and NF-kappaB Pathways.
LID - 10.3390/molecules24234251 [doi]
LID - 4251
AB  - Functional oligosaccharides, particularly curdlan (1-->3)-beta-d-glucan 
      oligosaccharides (GOS), play important roles in modulating host immune responses. 
      However, the molecular mechanisms underlying the immunostimulatory effects of GOS 
      on macrophage polarization are not clear. In this work, GOS (5-1000 microg/mL) were 
      non-toxic to bone marrow-derived macrophages (BMDMs) with improved pinocytic and 
      bactericidal capacities. Incubation with GOS (100 microg/mL) induced M1 phenotype 
      polarization of BMDMs as evidenced by increased CD11c(+)/CD86(+) (10.1%) and M1 
      gene expression of inducible nitric oxide synthase, interleukin (IL)-1beta, and 
      chemokine C-C-motif ligand 2. Accordingly, the secretion of cytokines IL-1beta, 
      IL-6, monocyte chemotactic protein-1, and tumor necrosis factor-alpha, as well as the 
      nitrite release of BMDMs were increased by GOS (100 microg/mL). Expression of 
      mitogen-activated protein kinases (MAPKs) of phosphorylated (p)-c-Jun 
      amino-terminal kinase, p-extracellular signal regulated kinase, and p-p38 in 
      BMDMs were increased by GOS, as well as the p-Stat1. Moreover, nuclear 
      factor-kappa B (NF-kappaB) p-p65 expression in BMDMs was promoted by GOS while it 
      suppressed IkappaBalpha expression. Receptor blocking with anti-CR3 (CD11b/CD18) and 
      anti-toll-like receptor (TLR) 2 antibodies diminished GOS induced M1 phenotype 
      polarization with reduced mRNA expression of M1 genes, decreased cytokine and 
      nitrite releases, and suppressed signaling pathway activation. Thus, CR3 
      (CD11b/CD18) and TLR2 mediated activation of MAPKs and NF-kappaB pathways are 
      responsible for GOS induced polarization of BMDMs.
FAU - Liu, Jun
AU  - Liu J
AD  - Beijing Advanced Innovation Center for Food Nutrition and Human Health, College 
      of Food Science and Nutritional Engineering, China Agricultural University, 
      Beijing 100083, China.
AD  - Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing 
      Technology and Business University, Beijing 100048, China.
AD  - Key Laboratory of Food Bioengineering (China National Light Industry), College of 
      Food Science and Nutritional Engineering, China Agricultural University, No.17 
      Qinghua Donglu, Haidian District, Beijing 100083, China.
FAU - Tang, Jiqing
AU  - Tang J
AD  - Beijing Advanced Innovation Center for Food Nutrition and Human Health, College 
      of Food Science and Nutritional Engineering, China Agricultural University, 
      Beijing 100083, China.
FAU - Li, Xiuting
AU  - Li X
AUID- ORCID: 0000-0002-3364-8191
AD  - Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing 
      Technology and Business University, Beijing 100048, China.
FAU - Yan, Qiaojuan
AU  - Yan Q
AD  - Bioresource Utilization Laboratory, College of Engineering, China Agricultural 
      University, Beijing 100083, China.
FAU - Ma, Junwen
AU  - Ma J
AD  - Beijing Advanced Innovation Center for Food Nutrition and Human Health, College 
      of Food Science and Nutritional Engineering, China Agricultural University, 
      Beijing 100083, China.
FAU - Jiang, Zhengqiang
AU  - Jiang Z
AD  - Beijing Advanced Innovation Center for Food Nutrition and Human Health, College 
      of Food Science and Nutritional Engineering, China Agricultural University, 
      Beijing 100083, China.
AD  - Key Laboratory of Food Bioengineering (China National Light Industry), College of 
      Food Science and Nutritional Engineering, China Agricultural University, No.17 
      Qinghua Donglu, Haidian District, Beijing 100083, China.
LA  - eng
GR  - 31630096/National Natural Science Foundation of China/
GR  - 2017YFD0400204/The National Key Research and Development Program of China/
PT  - Journal Article
DEP - 20191122
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 0 (Cytokines)
RN  - 0 (NF-kappa B)
RN  - 0 (Oligosaccharides)
RN  - 0 (beta-Glucans)
RN  - 6930DL209R (curdlan)
SB  - IM
MH  - Alcaligenes faecalis
MH  - Animals
MH  - Cell Polarity/drug effects
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Cytokines/genetics
MH  - Gene Expression Regulation/drug effects
MH  - MAP Kinase Signaling System/*drug effects
MH  - Macrophage Activation
MH  - Macrophages/*cytology/drug effects/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - NF-kappa B/*metabolism
MH  - Oligosaccharides/chemical synthesis/chemistry/*pharmacology
MH  - Phosphorylation
MH  - Pinocytosis/drug effects
MH  - Salmonella typhimurium/drug effects
MH  - beta-Glucans/*chemistry/pharmacology
PMC - PMC6930549
OTO - NOTNLM
OT  - curdlan (1-->3)-beta-d-glucan oligosaccharides
OT  - macrophage polarization
OT  - mitogen-activated protein kinases
OT  - nuclear factor-kappa B
OT  - receptor blocking
COIS- The authors declare no conflicts of interest.
EDAT- 2019/11/27 06:00
MHDA- 2020/04/09 06:00
PMCR- 2019/11/22
CRDT- 2019/11/27 06:00
PHST- 2019/10/23 00:00 [received]
PHST- 2019/11/15 00:00 [revised]
PHST- 2019/11/20 00:00 [accepted]
PHST- 2019/11/27 06:00 [entrez]
PHST- 2019/11/27 06:00 [pubmed]
PHST- 2020/04/09 06:00 [medline]
PHST- 2019/11/22 00:00 [pmc-release]
AID - molecules24234251 [pii]
AID - molecules-24-04251 [pii]
AID - 10.3390/molecules24234251 [doi]
PST - epublish
SO  - Molecules. 2019 Nov 22;24(23):4251. doi: 10.3390/molecules24234251.