PMID- 31767080
OWN - NLM
STAT- MEDLINE
DCOM- 20200211
LR  - 20200211
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 239
DP  - 2019 Dec
TI  - Effect of challenge dose of plasmid-mediated extended-spectrum beta-lactamase and 
      AmpC beta-lactamase producing Escherichia coli on time-until-colonization and level 
      of excretion in young broilers.
PG  - 108446
LID - S0378-1135(19)30782-5 [pii]
LID - 10.1016/j.vetmic.2019.108446 [doi]
AB  - Plasmid-mediated extended-spectrum beta-lactamase and AmpC beta-lactamase (ESBL/pAmpC) 
      producing bacteria are present at all levels of the broiler production pyramid. 
      Young birds can be found positive for ESBL/pAmpC-producing Escherichia coli 
      shortly after arrival at farm. The aim of this study was to determine the effect 
      of different challenge doses of ESBL/pAmpC-producing E. coli on 
      time-until-colonization and the level of excretion in young broilers. One-day-old 
      broilers (specific-pathogen free (SPF) and conventional Ross 308) were housed in 
      isolators and challenged with 0.5 ml ESBL/pAmpC-producing E. coli strains of 
      varying doses (10(1)-10(5) CFU/ml). Presence and concentration (CFU/gram feces) 
      of ESBL/pAmpC-producing E. coli and total E. coli were determined longitudinally 
      from cloacal swabs, and in cecal content 72 h after challenge. Higher challenge 
      doses resulted in shorter time-until-colonization. However, even the lowest dose 
      (10(1) CFU/ml) resulted in colonization of the broilers which excreted 
      >10(6) CFU/gram feces 72 h after inoculation. Conventional broilers were 
      colonized later than SPF broilers, although within 72 h after challenge all 
      broilers were excreting ESBL/pAmpC-producing E. coli. A probabilistic model was 
      used to estimate the probability of colonization by initial inoculation or 
      transmission. The higher the dose the higher the probability of excreting 
      ESBL/pAmpC-producing E. coli as a result of inoculation. In conclusion, low 
      initial doses of ESBL/pAmpC-producing E. coli can result in rapid colonization of 
      a flock. Interventions should thus be aimed to eliminate ESBL/pAmpC-producing 
      bacteria in the environment of the hatchlings and measures focusing at reducing 
      colonization and transmission of ESBL/pAmpC-producing E. coli should be applied 
      shortly after hatching.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Dame-Korevaar, Anita
AU  - Dame-Korevaar A
AD  - Department of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht 
      University, Utrecht, the Netherlands. Electronic address: 
      m.a.damekorevaar@gmail.com.
FAU - Fischer, Egil A J
AU  - Fischer EAJ
AD  - Department of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht 
      University, Utrecht, the Netherlands.
FAU - van der Goot, Jeanet
AU  - van der Goot J
AD  - Department of Bacteriology and Epidemiology, Wageningen Bioveterinary Research, 
      Lelystad, the Netherlands.
FAU - Velkers, Francisca
AU  - Velkers F
AD  - Department of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht 
      University, Utrecht, the Netherlands.
FAU - van den Broek, Jan
AU  - van den Broek J
AD  - Department of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht 
      University, Utrecht, the Netherlands.
FAU - Veldman, Kees
AU  - Veldman K
AD  - Department of Bacteriology and Epidemiology, Wageningen Bioveterinary Research, 
      Lelystad, the Netherlands.
FAU - Ceccarelli, Daniela
AU  - Ceccarelli D
AD  - Department of Bacteriology and Epidemiology, Wageningen Bioveterinary Research, 
      Lelystad, the Netherlands.
FAU - Mevius, Dik
AU  - Mevius D
AD  - Department of Bacteriology and Epidemiology, Wageningen Bioveterinary Research, 
      Lelystad, the Netherlands; Department of Infectious Diseases and Immunology, 
      Faculty of Veterinary Medicine, Utrecht University, Utrecht, the Netherlands.
FAU - Stegeman, Arjan
AU  - Stegeman A
AD  - Department of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht 
      University, Utrecht, the Netherlands.
LA  - eng
PT  - Journal Article
DEP - 20191004
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Bacterial Proteins)
RN  - EC 3.5.2.6 (AmpC beta-lactamases)
RN  - EC 3.5.2.6 (beta-Lactamases)
SB  - IM
MH  - Animals
MH  - Bacterial Proteins/genetics/metabolism
MH  - Chickens
MH  - Escherichia coli/enzymology/genetics
MH  - Escherichia coli Infections/enzymology/genetics/microbiology/*veterinary
MH  - Plasmids/genetics
MH  - Poultry Diseases/enzymology/genetics/*microbiology/transmission
MH  - Time
MH  - beta-Lactamases/genetics/metabolism
OTO - NOTNLM
OT  - Animal model
OT  - Antibiotic resistance
OT  - Dose-response
OT  - Inoculation
OT  - Poultry
OT  - Transmission
EDAT- 2019/11/27 06:00
MHDA- 2020/02/12 06:00
CRDT- 2019/11/27 06:00
PHST- 2019/07/02 00:00 [received]
PHST- 2019/08/21 00:00 [revised]
PHST- 2019/10/03 00:00 [accepted]
PHST- 2019/11/27 06:00 [entrez]
PHST- 2019/11/27 06:00 [pubmed]
PHST- 2020/02/12 06:00 [medline]
AID - S0378-1135(19)30782-5 [pii]
AID - 10.1016/j.vetmic.2019.108446 [doi]
PST - ppublish
SO  - Vet Microbiol. 2019 Dec;239:108446. doi: 10.1016/j.vetmic.2019.108446. Epub 2019 
      Oct 4.