PMID- 31767186
OWN - NLM
STAT- MEDLINE
DCOM- 20210126
LR  - 20210126
IS  - 1879-3231 (Electronic)
IS  - 0093-691X (Linking)
VI  - 147
DP  - 2020 Apr 15
TI  - Influence of the age of the individual on the stability of boar sperm genetic 
      material.
PG  - 176-182
LID - S0093-691X(19)30518-7 [pii]
LID - 10.1016/j.theriogenology.2019.11.018 [doi]
AB  - Routine evaluation of the sperm of livestock animals involves detection of 
      morphological abnormalities. However, most sperm defects that reduce fertilizing 
      capacity are a result of anomalies in spermatogenesis. The aim of the study was 
      to evaluate the effect of a boar's age on the stability of the genetic material 
      of its sperm. The age of the boar was found to have a significant effect on sperm 
      DNA stability and chromatin structure. The highest percentage of spermatozoa with 
      DNA fragmentation was found in the oldest group of boars (0,61%), while the 
      highest proportion of spermatozoa with abnormal histone retention (8,01%) and 
      protamination (9,78%) was found in the youngest group of boars. Aniline blue 
      (AB), chromomycin A3 (CMA3) and acridine orange (AO) staining should be routinely 
      used in individuals used for artificial insemination especially young animals at 
      the start of their exploitation for breeding, as well as older individuals with 
      an age-related decrease in the stability of genetic material. Earlier diagnosis 
      based on additional tests would allow for stricter selection and elimination of 
      males with fertility disorders from breeding, to be replaced by breeders of full 
      value. It was also demonstrated that all three staining methods mentioned above 
      can be used in classical morphological analysis, because they clearly distinguish 
      the sperm head from the background of the slide. Chromomycin staining clearly 
      reveals the midpiece and thus can be used as a specific staining method for its 
      evaluation. Staining with aniline blue is a fast and simple test whose result can 
      be analysed under a light microscope. This staining technique can be recommended 
      for use at insemination stations.
CI  - Copyright (c) 2019 Elsevier Inc. All rights reserved.
FAU - Czubaszek, Magdalena
AU  - Czubaszek M
AD  - Department of Animal Genetics and Horse Breeding, Siedlce University of Natural 
      Sciences and Humanities, Institute of Bioengineering and Animal Breeding, 14 
      Prusa Str, 08-110, Siedlce, Poland.
FAU - Andraszek, Katarzyna
AU  - Andraszek K
AD  - Department of Animal Genetics and Horse Breeding, Siedlce University of Natural 
      Sciences and Humanities, Institute of Bioengineering and Animal Breeding, 14 
      Prusa Str, 08-110, Siedlce, Poland. Electronic address: 
      katarzyna.andraszek@uph.edu.pl.
FAU - Banaszewska, Dorota
AU  - Banaszewska D
AD  - Department of Breeding Methods and Poultry Breeding, Siedlce University of 
      Natural Sciences and Humanities, Institute of Bioengineering and Animal Breeding, 
      14 Prusa Str, 08-110, Siedlce, Poland.
LA  - eng
PT  - Journal Article
DEP - 20191118
PL  - United States
TA  - Theriogenology
JT  - Theriogenology
JID - 0421510
RN  - 0 (Chromatin)
RN  - 0 (Fluorescent Dyes)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Aging/*physiology
MH  - Animals
MH  - Chromatin/*physiology
MH  - DNA/*physiology
MH  - DNA Fragmentation
MH  - Fluorescent Dyes
MH  - Male
MH  - Spermatogenesis
MH  - Spermatozoa/*physiology
MH  - Staining and Labeling/veterinary
MH  - Swine/*physiology
OTO - NOTNLM
OT  - Age
OT  - Boar
OT  - Histone
OT  - Protamine
OT  - Sperm
EDAT- 2019/11/27 06:00
MHDA- 2021/01/27 06:00
CRDT- 2019/11/27 06:00
PHST- 2019/01/24 00:00 [received]
PHST- 2019/11/12 00:00 [revised]
PHST- 2019/11/17 00:00 [accepted]
PHST- 2019/11/27 06:00 [pubmed]
PHST- 2021/01/27 06:00 [medline]
PHST- 2019/11/27 06:00 [entrez]
AID - S0093-691X(19)30518-7 [pii]
AID - 10.1016/j.theriogenology.2019.11.018 [doi]
PST - ppublish
SO  - Theriogenology. 2020 Apr 15;147:176-182. doi: 
      10.1016/j.theriogenology.2019.11.018. Epub 2019 Nov 18.