PMID- 31767456
OWN - NLM
STAT- MEDLINE
DCOM- 20200320
LR  - 20210816
IS  - 1090-2414 (Electronic)
IS  - 0147-6513 (Linking)
VI  - 189
DP  - 2020 Feb
TI  - The steroid receptor coactivator 1 (SRC1) and 3 (SRC3) recruitment as a novel 
      molecular initiating event of 4-n-nonylphenol in estrogen receptor alpha-mediated 
      pathways.
PG  - 109958
LID - S0147-6513(19)31289-8 [pii]
LID - 10.1016/j.ecoenv.2019.109958 [doi]
AB  - Recently, the action of steroid receptor coactivators (SRCs) has been recognized 
      to be an important molecular initiating event (MIE) in estrogenic adverse outcome 
      pathways (AOPs). However, the role of SRCs in the molecular mechanisms of many 
      highly concerned environmental estrogens remains poorly understood. In this 
      study, the widely studied environmental estrogen, 4-n-nonylphenol (4-n-NP), was 
      used as a typical pollutant to study SRCs recruitment in its estrogenic effects. 
      In MCF7 cell proliferation (E-SCREEN) assay and MVLN cell assay, 4-n-NP showed 
      significant estrogenic potency that involved an increase in estrogen receptor alpha 
      (ERalpha), SRC1 and SRC3 transcript levels. Moreover, 4-n-NP was found to induce 
      estrogen response element (ERE)-mediated activity via ERalpha in MVLN cells. To 
      investigate the mechanism by which SRCs recruitment is induced by 4-n-NP-ERalpha, a 
      coactivators recruitment assay was performed, and the results showed that 
      4-n-NP-ERalpha recruited both SRC1 and SRC3, whereas it failed to recruit SRC2. 
      Similarly, it had no interaction with SRC2 in the ERalpha-SRC2 two-hybrid yeast 
      assay. This is the first report to investigate the novel MIE of SRCs recruitment 
      in 4-n-NP-ERalpha-induced estrogenicity. Overall, our results suggest that the action 
      of 4-n-NP on estrogenic effects involves the following MIEs: the activation of 
      ERalpha, the recruitment of SRC1 and SRC3, and the induction of ERE-mediated 
      activity. The findings also provide valuable insights into the MIE associated 
      with the different SRCs that are recruited in the adverse outcome pathways of 
      environmental estrogens.
CI  - Copyright (c) 2019 Elsevier Inc. All rights reserved.
FAU - Ji, Xiaoya
AU  - Ji X
AD  - Key Laboratory of Drinking Water Science and Technology, Research Center for 
      Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China; 
      College of Resources and Environment, University of Chinese Academy of Sciences, 
      Beijing, 100049, China.
FAU - Li, Na
AU  - Li N
AD  - Key Laboratory of Drinking Water Science and Technology, Research Center for 
      Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China; 
      College of Resources and Environment, University of Chinese Academy of Sciences, 
      Beijing, 100049, China.
FAU - Yang, Rong
AU  - Yang R
AD  - Beijing Water Quality Monitoring Center for South-to-North Water Diversion, 
      Beijing, 100093, China.
FAU - Rao, Kaifeng
AU  - Rao K
AD  - Key Laboratory of Drinking Water Science and Technology, Research Center for 
      Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China.
FAU - Ma, Mei
AU  - Ma M
AD  - Key Laboratory of Drinking Water Science and Technology, Research Center for 
      Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China; 
      College of Resources and Environment, University of Chinese Academy of Sciences, 
      Beijing, 100049, China. Electronic address: mamei@rcees.ac.cn.
FAU - Wang, Zijian
AU  - Wang Z
AD  - Key Laboratory of Drinking Water Science and Technology, Research Center for 
      Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China.
LA  - eng
PT  - Journal Article
DEP - 20191122
PL  - Netherlands
TA  - Ecotoxicol Environ Saf
JT  - Ecotoxicology and environmental safety
JID - 7805381
RN  - 0 (ESR1 protein, human)
RN  - 0 (Environmental Pollutants)
RN  - 0 (Estrogen Receptor alpha)
RN  - 0 (Estrogens)
RN  - 0 (Phenols)
RN  - EC 2.3.1.48 (NCOA1 protein, human)
RN  - EC 2.3.1.48 (NCOA3 protein, human)
RN  - EC 2.3.1.48 (Nuclear Receptor Coactivator 1)
RN  - EC 2.3.1.48 (Nuclear Receptor Coactivator 3)
RN  - I03GBV4WEL (4-nonylphenol)
SB  - IM
MH  - Cell Proliferation/drug effects
MH  - Environmental Pollutants/*pharmacology
MH  - Estrogen Receptor alpha/*metabolism
MH  - Estrogens/*pharmacology
MH  - Humans
MH  - MCF-7 Cells
MH  - Nuclear Receptor Coactivator 1/*metabolism
MH  - Nuclear Receptor Coactivator 3/*metabolism
MH  - Phenols/*pharmacology
OTO - NOTNLM
OT  - 4-n-Nonylphenol
OT  - Estrogen receptor alpha (ERalpha)
OT  - Estrogenic activity
OT  - Molecular initiating event (MIE)
OT  - Steroid receptor coactivator (SRC)
EDAT- 2019/11/27 06:00
MHDA- 2020/03/21 06:00
CRDT- 2019/11/27 06:00
PHST- 2019/09/26 00:00 [received]
PHST- 2019/11/10 00:00 [revised]
PHST- 2019/11/12 00:00 [accepted]
PHST- 2019/11/27 06:00 [pubmed]
PHST- 2020/03/21 06:00 [medline]
PHST- 2019/11/27 06:00 [entrez]
AID - S0147-6513(19)31289-8 [pii]
AID - 10.1016/j.ecoenv.2019.109958 [doi]
PST - ppublish
SO  - Ecotoxicol Environ Saf. 2020 Feb;189:109958. doi: 10.1016/j.ecoenv.2019.109958. 
      Epub 2019 Nov 22.