PMID- 31767544
OWN - NLM
STAT- MEDLINE
DCOM- 20201021
LR  - 20201021
IS  - 1878-1705 (Electronic)
IS  - 1567-5769 (Linking)
VI  - 78
DP  - 2020 Jan
TI  - Schisandrin A protects against lipopolysaccharide-induced mastitis through 
      activating Nrf2 signaling pathway and inducing autophagy.
PG  - 105983
LID - S1567-5769(19)31824-7 [pii]
LID - 10.1016/j.intimp.2019.105983 [doi]
AB  - Schisandrin A (Sch A), a dibenzocyclooctadiene lignan extracted from Schisandra 
      chinensis (Turcz.) Baill., has anti-oxidant and anti-inflammatory effects, but 
      the effect on masitits has not been studied. Therefore, we investigated the 
      effect of Sch A in cell and mouse models of lipopolysaccharide (LPS)-induced 
      mastitis. Studies in vivo showed that Sch A reduced LPS-induced mammary injury 
      and the production of pro-inflammatory mediators. Sch A also decreased the levels 
      of pro-inflammatory mediators and activated nuclear factor-E2 associated factor 2 
      (Nrf2) signaling pathway in mouse mammary epithelial cells (mMECs). The Nrf2 
      inhibitor partially abrogated the downregulation of Sch A on LPS-induced 
      inflammatory response. In addition, LPS stimulation suppressed autophagy, while 
      both Sch A and the autophagy inducer rapamycin activated autophagy in mMECs, 
      which down-regulated inflammatory response. Sch A also restrained LPS-induced 
      phosphorylation of mammalian target of rapamycin (mTOR) and activated 
      AMP-activated protein kinase (AMPK) and unc-51 like kinase 1 (ULK1). In summary, 
      these results suggest that Sch A exerts protective effects in LPS-induced 
      mastitis models by activating Nrf2 signaling pathway and inducing autophagy and 
      the autophagy is initiated by suppressing mTOR signaling pathway and activating 
      AMPK-ULK1 signaling pathway.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Xu, Dianwen
AU  - Xu D
AD  - College of Veterinary Medicine, Jilin University, Changchun 130062, Jilin, China.
FAU - Liu, Juxiong
AU  - Liu J
AD  - College of Veterinary Medicine, Jilin University, Changchun 130062, Jilin, China.
FAU - Ma, He
AU  - Ma H
AD  - College of Veterinary Medicine, Jilin University, Changchun 130062, Jilin, China.
FAU - Guo, Wenjin
AU  - Guo W
AD  - College of Veterinary Medicine, Jilin University, Changchun 130062, Jilin, China.
FAU - Wang, Jiaxin
AU  - Wang J
AD  - College of Veterinary Medicine, Jilin University, Changchun 130062, Jilin, China.
FAU - Kan, Xingchi
AU  - Kan X
AD  - College of Veterinary Medicine, Jilin University, Changchun 130062, Jilin, China.
FAU - Li, Yanwei
AU  - Li Y
AD  - College of Veterinary Medicine, Jilin University, Changchun 130062, Jilin, China.
FAU - Gong, Qian
AU  - Gong Q
AD  - College of Veterinary Medicine, Jilin University, Changchun 130062, Jilin, China.
FAU - Cao, Yu
AU  - Cao Y
AD  - College of Veterinary Medicine, Jilin University, Changchun 130062, Jilin, China.
FAU - Cheng, Ji
AU  - Cheng J
AD  - College of Veterinary Medicine, Jilin University, Changchun 130062, Jilin, China.
FAU - Fu, Shoupeng
AU  - Fu S
AD  - College of Veterinary Medicine, Jilin University, Changchun 130062, Jilin, China. 
      Electronic address: fushoupeng@jlu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20191122
PL  - Netherlands
TA  - Int Immunopharmacol
JT  - International immunopharmacology
JID - 100965259
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Cyclooctanes)
RN  - 0 (IL1B protein, mouse)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Lignans)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (Nfe2l2 protein, mouse)
RN  - 0 (Polycyclic Compounds)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 74XQL5DO3S (schizandrin A)
RN  - EC 1.11.1.7 (Peroxidase)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/pharmacology/*therapeutic use
MH  - Autophagy/*drug effects
MH  - Cells, Cultured
MH  - Cyclooctanes/pharmacology/*therapeutic use
MH  - Epithelial Cells/drug effects/metabolism
MH  - Female
MH  - Interleukin-1beta/genetics
MH  - Lignans/pharmacology/*therapeutic use
MH  - Lipopolysaccharides
MH  - Mammary Glands, Animal/drug effects
MH  - Mastitis/chemically induced/*drug therapy/metabolism/pathology
MH  - Mice, Inbred C57BL
MH  - NF-E2-Related Factor 2/*metabolism
MH  - Peroxidase/metabolism
MH  - Polycyclic Compounds/pharmacology/*therapeutic use
MH  - Signal Transduction/drug effects
MH  - Tumor Necrosis Factor-alpha/genetics
OTO - NOTNLM
OT  - AMPK-ULK1
OT  - Autophagy
OT  - Mastitis
OT  - Nrf2
OT  - Schisandrin A
OT  - mTOR
EDAT- 2019/11/27 06:00
MHDA- 2020/10/22 06:00
CRDT- 2019/11/27 06:00
PHST- 2019/08/16 00:00 [received]
PHST- 2019/10/11 00:00 [revised]
PHST- 2019/10/14 00:00 [accepted]
PHST- 2019/11/27 06:00 [pubmed]
PHST- 2020/10/22 06:00 [medline]
PHST- 2019/11/27 06:00 [entrez]
AID - S1567-5769(19)31824-7 [pii]
AID - 10.1016/j.intimp.2019.105983 [doi]
PST - ppublish
SO  - Int Immunopharmacol. 2020 Jan;78:105983. doi: 10.1016/j.intimp.2019.105983. Epub 
      2019 Nov 22.