PMID- 31770592
OWN - NLM
STAT- MEDLINE
DCOM- 20201106
LR  - 20201106
IS  - 1769-714X (Electronic)
IS  - 1286-4579 (Linking)
VI  - 22
IP  - 3
DP  - 2020 Apr
TI  - Enhanced expression of NLRP3 inflammasome components by monocytes of patients 
      with pulmonary paracoccidioidomycosis is associated with smoking and 
      intracellular hypoxemia.
PG  - 137-143
LID - S1286-4579(19)30165-0 [pii]
LID - 10.1016/j.micinf.2019.11.001 [doi]
AB  - Paracoccidioidomycosis (PCM) is a systemic mycosis caused by thermally dimorphic 
      fungi of the genus Paracoccidioides that affects predominantly 30-60-year-old 
      male rural workers. The main clinical forms of the disease are acute/subacute, 
      chronic (CF); almost all CF patients develop pulmonary fibrosis, and they also 
      exhibit emphysema due to smoke. An important cytokine in this context, IL-1beta, 
      different from the others, is produced by an intracellular multimolecular complex 
      called inflammasome that is activated by pathogens and/or host signs of damage. 
      Inflammasome has been recognized for its contribution to chronic inflammatory 
      diseases, from that, we hypothesized that this activation could be involved in 
      paracoccidioidomycosis, contributing to chronic inflammation. While inflammasome 
      activation has been demonstrated in experimental models of Paracoccidioides 
      brasiliensis infection, no information is available in patients, leading us to 
      investigate the participation of NLRP3-inflammasome machinery in CF/PCM patients 
      from a Brazilian endemic area. Our findings showed increased priming in mRNA 
      levels of NLRP3 inflammasome genes by monocytes of PCM patients in vitro than 
      healthy controls. Similar intracellular protein expression of NLRP3, CASP-1, ASC, 
      and IL-1beta were also observed in freshly isolated monocytes of PCM patients and 
      smoker controls. Increased expression of NLRP3 and ASC was observed in monocytes 
      from PCM patients under hypoxia in comparison with smoker controls. For the first 
      time, we showed that primed monocytes of CF-PCM patients were associated with 
      enhanced expression of components of NLRP3-inflammasome due to smoke. Also, 
      hypoxemia boosted this machinery. These findings reinforce the systemic low-grade 
      inflammation activation observed in PCM during and after treatment.
CI  - Copyright (c) 2019 Institut Pasteur. Published by Elsevier Masson SAS. All rights 
      reserved.
FAU - Amorim, Barbara Casella
AU  - Amorim BC
AD  - Sao Paulo State University (UNESP), School of Sciences, Bauru, SP, Brazil; Sao 
      Paulo State University (UNESP), Medical School, Botucatu, SP, Brazil.
FAU - Pereira-Latini, Ana Carla
AU  - Pereira-Latini AC
AD  - Lauro de Souza Lima Institute, Bauru, SP, Brazil.
FAU - Golim, Marjorie de Assis
AU  - Golim MA
AD  - Sao Paulo State University (UNESP), Medical School, Botucatu, SP, Brazil.
FAU - Ruiz Junior, Raul Lopes
AU  - Ruiz Junior RL
AD  - Sao Paulo State University (UNESP), Medical School, Botucatu, SP, Brazil.
FAU - Yoo, Hugo Hyung Bok
AU  - Yoo HHB
AD  - Sao Paulo State University (UNESP), Medical School, Botucatu, SP, Brazil.
FAU - Arruda, Maria Sueli Parreira de
AU  - Arruda MSP
AD  - Sao Paulo State University (UNESP), School of Sciences, Bauru, SP, Brazil.
FAU - Tavares, Aldo Henrique
AU  - Tavares AH
AD  - University of Brasilia (UnB), Institute of Biological Sciences Brasilia, Federal 
      District, Brazil.
FAU - Cavalcante, Ricardo de Souza
AU  - Cavalcante RS
AD  - Sao Paulo State University (UNESP), Medical School, Botucatu, SP, Brazil.
FAU - Mendes, Rinaldo Poncio
AU  - Mendes RP
AD  - Sao Paulo State University (UNESP), Medical School, Botucatu, SP, Brazil.
FAU - Pontillo, Alessandra
AU  - Pontillo A
AD  - University of Sao Paulo (USP), Institute of Biomedical Sciences, SP, Brazil.
FAU - Venturini, James
AU  - Venturini J
AD  - Sao Paulo State University (UNESP), Medical School, Botucatu, SP, Brazil; Federal 
      University of Mato Grosso do Sul (UFMS), Medical School, Campo Grande, MS, 
      Brazil. Electronic address: james.venturini@ufms.br.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191123
PL  - France
TA  - Microbes Infect
JT  - Microbes and infection
JID - 100883508
RN  - 0 (NLR Family, Pyrin Domain-Containing 3 Protein)
RN  - 0 (NLRP3 protein, human)
SB  - IM
MH  - Cell Hypoxia
MH  - Humans
MH  - Invasive Fungal Infections/immunology/microbiology
MH  - Lung Diseases, Fungal/microbiology
MH  - Monocytes/*immunology/microbiology
MH  - NLR Family, Pyrin Domain-Containing 3 Protein/*genetics/immunology
MH  - Paracoccidioides
MH  - Paracoccidioidomycosis/*immunology/microbiology
MH  - Pulmonary Fibrosis/immunology/microbiology
MH  - *Smoking
OTO - NOTNLM
OT  - Antifungal therapy
OT  - Hypoxia
OT  - Inflammasome
OT  - Monocytes
OT  - Paracoccidioidomycosis
OT  - Pulmonary fibrosis
COIS- Declaration of Competing Interest We declare that the manuscript does not present 
      any potential conflicts of interest.
EDAT- 2019/11/27 06:00
MHDA- 2020/11/11 06:00
CRDT- 2019/11/27 06:00
PHST- 2019/07/05 00:00 [received]
PHST- 2019/11/14 00:00 [revised]
PHST- 2019/11/15 00:00 [accepted]
PHST- 2019/11/27 06:00 [pubmed]
PHST- 2020/11/11 06:00 [medline]
PHST- 2019/11/27 06:00 [entrez]
AID - S1286-4579(19)30165-0 [pii]
AID - 10.1016/j.micinf.2019.11.001 [doi]
PST - ppublish
SO  - Microbes Infect. 2020 Apr;22(3):137-143. doi: 10.1016/j.micinf.2019.11.001. Epub 
      2019 Nov 23.