PMID- 31770759 OWN - NLM STAT- MEDLINE DCOM- 20200210 LR - 20200210 IS - 1423-0097 (Electronic) IS - 1018-2438 (Linking) VI - 181 IP - 2 DP - 2020 TI - Identifying Potential Co-Sensitization and Cross-Reactivity Patterns Based on Component-Resolved Diagnosis. PG - 81-93 LID - 10.1159/000504320 [doi] AB - BACKGROUND: Component-Resolved diagnosis (CRD) can help to establish immunoglobulin E (IgE) sensitization profiles and potential risks and determines whether specific IgE is the result of primary sensitization or cross-reactivity, especially for those who are polysensitized. METHODS: We recruited 432 patients with mite-sensitized respiratory allergic diseases to study the co-sensitization and cross-reactivity of the 17 allergen components in Guangdong Province, China, using the CRD method and to describe the potential association between allergen components. RESULTS: Among the 432 patients, serum specific immunoglobulin E of the 17 components were tested by EUROIMMUN system. Der p 1 (81.48%), Der f 2 (77.78%), Der f 1 (74.07%), Der p 2 (66.20%) and Der p 23 (54.63%) were the main sensitized components in patients with mite-sensitized respiratory allergy, while the components of cockroach, crab, and shrimp had a lower positive rate. In the crude extract allergen-positive samples, Der f 2 (91.06%) and Der f 1 (86.72%) were the major sensitized components of Der f, while Der p 1 (94.52%), Der p 2 (78.36%), Der p 23 (63.29%) were the major sensitized components of Der p, And other components of Der p such as Der p 7 (34.25%), Der p 5 (17.81%), Der p 10 (12.05%), Der p 3 (1.92%) were all below 50.00%. Blo t 5 (54.55%) was one of the major components of Blo t. The positive rates of all Bla g components were as follows, rBla g 2 (15.56%) >rBla g 5 (8.89%) >rBla g 4 (4.44%) >rBla g 1 (1.11%). The positive rate of the only available pen a 1 component was 9.43%. Using hierarchical cluster and optimal scale analysis, 17 components can be roughly divided into 5 different sensitization clusters. Also, from the results of the Venn diagram, the allergen component in each cluster has a high proportion of co-sensitization and cross-reactivity. Regardless of age, total IgE levels, and disease type factors, similar sensitization profiles were observed for each component in the same category based on hierarchical clustering analysis. CONCLUSIONS: Epidemiological data on allergen components causing allergic symptoms can be further understood using CRD. Der p 1, Der p 2, Der p 23, Der f 1, Der f 2 as the primary sensitizing component of the study cohort. The positive rate for Blo t 5 was 28.01% for all populations and 54.45% for Blo t-positive samples. In addition, CRD allows us to identify more potential allergen associations such as common sensitivities and cross-reactions between component proteins. Based on these results, we suggest that when patients are identified as sensitized to a particular allergen, clinicians can pay more attention to other allergy components that are closely related to it. CI - (c) 2019 S. Karger AG, Basel. FAU - Huang, Zhifeng AU - Huang Z AD - Department of Allergy and Clinical Immunology, Guangzhou Institute of Respiratory health, State Key Laboratory of Respiratory Disease, National Clinical Research Center of Respiratory Disease, First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China. FAU - Zou, Xiangwei AU - Zou X AD - Department of Allergy and Clinical Immunology, Guangzhou Institute of Respiratory health, State Key Laboratory of Respiratory Disease, National Clinical Research Center of Respiratory Disease, First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China. FAU - Chen, Hao AU - Chen H AD - Department of Allergy and Clinical Immunology, Guangzhou Institute of Respiratory health, State Key Laboratory of Respiratory Disease, National Clinical Research Center of Respiratory Disease, First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China. FAU - Liao, Chenxi AU - Liao C AD - Department of Allergy and Clinical Immunology, Guangzhou Institute of Respiratory health, State Key Laboratory of Respiratory Disease, National Clinical Research Center of Respiratory Disease, First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China. FAU - Hu, Haisheng AU - Hu H AD - Department of Allergy and Clinical Immunology, Guangzhou Institute of Respiratory health, State Key Laboratory of Respiratory Disease, National Clinical Research Center of Respiratory Disease, First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China. FAU - Luo, Wenting AU - Luo W AD - Department of Allergy and Clinical Immunology, Guangzhou Institute of Respiratory health, State Key Laboratory of Respiratory Disease, National Clinical Research Center of Respiratory Disease, First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China. FAU - Sun, Baoqing AU - Sun B AD - Department of Allergy and Clinical Immunology, Guangzhou Institute of Respiratory health, State Key Laboratory of Respiratory Disease, National Clinical Research Center of Respiratory Disease, First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China, sunbaoqing@vip.163.com. LA - eng PT - Journal Article DEP - 20191126 PL - Switzerland TA - Int Arch Allergy Immunol JT - International archives of allergy and immunology JID - 9211652 RN - 0 (Allergens) RN - 37341-29-0 (Immunoglobulin E) SB - IM MH - Adolescent MH - Allergens/*immunology MH - Animals MH - Asthma/immunology MH - Brachyura/immunology MH - Child MH - China MH - Cockroaches/immunology MH - Cohort Studies MH - Cross Reactions/*immunology MH - Decapoda/immunology MH - Female MH - Humans MH - Immunoglobulin E/*immunology MH - Male MH - Mites/immunology MH - Rhinitis, Allergic/immunology OTO - NOTNLM OT - Allergy rhinitis OT - Asthma OT - Co-Sensitization OT - Component-resolved diagnosis OT - Cross-Reactivity OT - Dust mite sensitized EDAT- 2019/11/27 06:00 MHDA- 2020/02/11 06:00 CRDT- 2019/11/27 06:00 PHST- 2019/07/24 00:00 [received] PHST- 2019/10/23 00:00 [accepted] PHST- 2019/11/27 06:00 [pubmed] PHST- 2020/02/11 06:00 [medline] PHST- 2019/11/27 06:00 [entrez] AID - 000504320 [pii] AID - 10.1159/000504320 [doi] PST - ppublish SO - Int Arch Allergy Immunol. 2020;181(2):81-93. doi: 10.1159/000504320. Epub 2019 Nov 26.