PMID- 31771729
OWN - NLM
STAT- MEDLINE
DCOM- 20191220
LR  - 20200108
IS  - 2095-4352 (Print)
VI  - 31
IP  - 10
DP  - 2019 Oct
TI  - [Heparin inhibits lipopolysaccharide-induced adhesion of monocytes to endothelial 
      cells].
PG  - 1281-1284
LID - 10.3760/cma.j.issn.2095-4352.2019.10.019 [doi]
AB  - OBJECTIVE: To investigate the effects of heparin on the secretion of monocyte 
      chemotactic protein-1 (MCP-1) in human umbilical vein endothelial cells (HUVEC) 
      and the adhesion of monocytes to endothelial cells stimulated by 
      lipopolysaccharide (LPS). METHODS: HUVEC were cultured in vitro, and the cells 
      between generation 4 and 5 were used for the experiments. The cells were divided 
      into phosphate buffer saline (PBS) control group, heparin control group, LPS 
      group, and heparin+LPS group. The LPS group was challenged with LPS 10 mg/L; the 
      PBS control group was added with the same amount of PBS; the heparin group was 
      added with 10 kU/L unfractionated heparin; the heparin+LPS group was treated with 
      10 kU/L unfractionated heparin 15 minutes before LPS stimulation. The cells were 
      harvested at 6 hours and 12 hours after LPS stimulation in each group, and the 
      MCP-1 mRNA expression was determined by real-time fluorescent quantitative 
      reverse transcription-polymerase chain reaction (qRT-PCR). After incubation with 
      each group, the fluorescent dyelabeled human monocyte cell line THP-1 was 
      cultured with each group for 1 hour in the dark, and the adhesion density of 
      THP-1 and HUVEC was observed under fluorescence microscope. RESULTS: Compared 
      with the PBS control group, the MCP-1 mRNA expression significantly increased at 
      6 hours and 12 hours after LPS stimulation and peaked at 6 hours, then decreased 
      gradually, but remained significantly higher than the PBS control group at 12 
      hours [2(-DeltaDeltaCt): 16.41 (15.03, 18.00) vs. 1.00 (0.80, 1.26) at 6 hours, 9.27 
      (8.11, 9.85) vs. 1.00 (0.84, 1.20) at 12 hours, both P < 0.05]. Heparin 
      preconditioning significantly reduced LPS-induced MCP-1 mRNA expression 
      [2(-DeltaDeltaCt): 2.06 (1.72, 2.46) vs. 16.41 (15.03, 18.00) at 6 hours, 2.46 (2.19, 
      4.56) vs. 9.27 (8.11, 9.85) at 12 hours, both P < 0.05]. There was no significant 
      difference in MCP-1 mRNA expression between the heparin control group and the PBS 
      control group [2(-DeltaDeltaCt): 1.47 (1.29, 1.65) vs. 1.00 (0.80, 1.26) at 6 hours, 2.69 
      (2.58, 2.77) vs. 1.00 (0.84, 1.20) at 12 hours, both P > 0.05]. Fluorescence 
      microscopy observation showed that LPS stimulation could promote the adhesion of 
      THP-1 to HUVEC; heparin preconditioning could inhibit the adhesion of THP-1 to 
      HUVEC stimulated by LPS. CONCLUSIONS: Heparin preconditioning could inhibit the 
      MCP-1 mRNA expression , thereby reduce the adhesion of THP-1 to HUVEC, thus play 
      a protective role in sepsis.
FAU - Chen, Tianlu
AU  - Chen T
AD  - Department of Critical Care Medicine, the First Affiliated Hospital of China 
      Medical University, Shenyang 110001, Liaoning, China.
AD  - Department of Critical Care Medicine, Xi'an No.4 Hospital, Xi'an 710004, Shaanxi, 
      China. Corresponding author: Li Xu, Email: 13604059359@189.cn.
FAU - Ma, Xiaochun
AU  - Ma X
AD  - Department of Critical Care Medicine, the First Affiliated Hospital of China 
      Medical University, Shenyang 110001, Liaoning, China.
FAU - Li, Xu
AU  - Li X
AD  - Department of Critical Care Medicine, the First Affiliated Hospital of China 
      Medical University, Shenyang 110001, Liaoning, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
JT  - Zhonghua wei zhong bing ji jiu yi xue
JID - 101604552
RN  - 0 (Fibrinolytic Agents)
RN  - 0 (Lipopolysaccharides)
RN  - 9005-49-6 (Heparin)
SB  - IM
MH  - Cells, Cultured
MH  - Fibrinolytic Agents
MH  - *Heparin
MH  - Human Umbilical Vein Endothelial Cells
MH  - Humans
MH  - *Lipopolysaccharides
MH  - *Monocytes
EDAT- 2019/11/28 06:00
MHDA- 2019/12/21 06:00
CRDT- 2019/11/28 06:00
PHST- 2019/11/28 06:00 [entrez]
PHST- 2019/11/28 06:00 [pubmed]
PHST- 2019/12/21 06:00 [medline]
AID - 10.3760/cma.j.issn.2095-4352.2019.10.019 [doi]
PST - ppublish
SO  - Zhonghua Wei Zhong Bing Ji Jiu Yi Xue. 2019 Oct;31(10):1281-1284. doi: 
      10.3760/cma.j.issn.2095-4352.2019.10.019.