PMID- 31772706
OWN - NLM
STAT- MEDLINE
DCOM- 20200410
LR  - 20200410
IS  - 1942-0994 (Electronic)
IS  - 1942-0900 (Print)
IS  - 1942-0994 (Linking)
VI  - 2019
DP  - 2019
TI  - Apocynin Dietary Supplementation Delays Mouse Ovarian Ageing.
PG  - 5316984
LID - 10.1155/2019/5316984 [doi]
LID - 5316984
AB  - Advanced maternal age is associated with higher infertility rates, 
      pregnancy-associated complications, and progeny health issues. The ovary is 
      considered the main responsible for these consequences due to a continuous decay 
      in follicle number and oocyte quality. Intracellular imbalance between oxidant 
      molecules and antioxidant mechanisms, in favour of the former, results in 
      oxidative stress (OS) that is believed to contribute to ovarian ageing. This work 
      is aimed at evaluating whether an age-related increase in ovarian OS, 
      inflammation, and fibrosis may contribute to tissue dysfunction and whether 
      specific antioxidant supplementation with a NADPH oxidase inhibitor (apocynin) 
      could ameliorate them. Mice aged 8-12 weeks (reproductively young) or 38-42 weeks 
      (reproductively aged) were employed. Aged mice were divided into two groups, with 
      one receiving apocynin (5 mM) in the drinking water, for 7 weeks, upon which 
      animals were sacrificed and their ovaries collected. Ovarian structure was 
      similar at both ages, but the ovaries from reproductively aged mice exhibited 
      lipofuscin deposition, enhanced fibrosis, and a significant age-related reduction 
      in primordial and primary follicle number when compared to younger animals. 
      Protein carbonylation and nitration, and markers of OS were significantly 
      increased with age. Moreover, mRNA levels of inflammation markers, collagens, 
      metalloproteinases (MMPs), and tissue inhibitor MMPs (TIMPs) were upregulated. 
      Expression of the antifibrotic miRNA29c-3p was significantly reduced. Apocynin 
      supplementation ameliorated most of the age-related observed changes, sometimes 
      to values similar to those observed in young females. These findings indicate 
      that there is an age-related increase in OS that plays an important role in 
      enhancing inflammation and collagen deposition, contributing to a decline in 
      female fertility. Apocynin supplementation suggests that the imbalance can be 
      ameliorated and thus delay ovarian ageing harmful effects.
CI  - Copyright (c) 2019 F. Timoteo-Ferreira et al.
FAU - Timoteo-Ferreira, F
AU  - Timoteo-Ferreira F
AD  - Instituto de Biologia Molecular e Celular (IBMC) and Instituto de Inovacao e 
      Investigacao em Saude (I3S), Universidade do Porto, Portugal.
AD  - Unit of Experimental Biology, Department of Biomedicine, Faculty of Medicine of 
      Porto University, Portugal.
FAU - Mendes, S
AU  - Mendes S
AUID- ORCID: 0000-0003-0002-0303
AD  - Instituto de Biologia Molecular e Celular (IBMC) and Instituto de Inovacao e 
      Investigacao em Saude (I3S), Universidade do Porto, Portugal.
AD  - Unit of Experimental Biology, Department of Biomedicine, Faculty of Medicine of 
      Porto University, Portugal.
FAU - Rocha, N A
AU  - Rocha NA
AD  - Unit of Experimental Biology, Department of Biomedicine, Faculty of Medicine of 
      Porto University, Portugal.
FAU - Matos, L
AU  - Matos L
AUID- ORCID: 0000-0003-4188-532X
AD  - Instituto de Biologia Molecular e Celular (IBMC) and Instituto de Inovacao e 
      Investigacao em Saude (I3S), Universidade do Porto, Portugal.
AD  - Unit of Experimental Biology, Department of Biomedicine, Faculty of Medicine of 
      Porto University, Portugal.
AD  - Faculty of Dental Medicine of Porto University, Portugal.
AD  - Faculty of Nutrition and Food Sciences of Porto University, Portugal.
FAU - Rodrigues, A R
AU  - Rodrigues AR
AUID- ORCID: 0000-0002-9613-1552
AD  - Instituto de Biologia Molecular e Celular (IBMC) and Instituto de Inovacao e 
      Investigacao em Saude (I3S), Universidade do Porto, Portugal.
AD  - Unit of Experimental Biology, Department of Biomedicine, Faculty of Medicine of 
      Porto University, Portugal.
FAU - Almeida, H
AU  - Almeida H
AUID- ORCID: 0000-0002-3036-3211
AD  - Instituto de Biologia Molecular e Celular (IBMC) and Instituto de Inovacao e 
      Investigacao em Saude (I3S), Universidade do Porto, Portugal.
AD  - Unit of Experimental Biology, Department of Biomedicine, Faculty of Medicine of 
      Porto University, Portugal.
AD  - Obstetrics-Gynecology, Hospital-CUF Porto, 4100 180 Porto, Portugal.
FAU - Silva, E
AU  - Silva E
AUID- ORCID: 0000-0002-6736-3469
AD  - Instituto de Biologia Molecular e Celular (IBMC) and Instituto de Inovacao e 
      Investigacao em Saude (I3S), Universidade do Porto, Portugal.
AD  - Unit of Experimental Biology, Department of Biomedicine, Faculty of Medicine of 
      Porto University, Portugal.
LA  - eng
PT  - Journal Article
DEP - 20191020
PL  - United States
TA  - Oxid Med Cell Longev
JT  - Oxidative medicine and cellular longevity
JID - 101479826
RN  - 0 (Acetophenones)
RN  - B6J7B9UDTR (acetovanillone)
SB  - IM
MH  - Acetophenones/pharmacology/*therapeutic use
MH  - Aged
MH  - Animals
MH  - Dietary Supplements
MH  - Female
MH  - Humans
MH  - Mice
MH  - Ovary/*drug effects
MH  - Pregnancy
MH  - Reproduction/*drug effects
PMC - PMC6854951
COIS- The authors declare that they have no conflicts of interest.
EDAT- 2019/11/28 06:00
MHDA- 2020/04/11 06:00
PMCR- 2019/10/20
CRDT- 2019/11/28 06:00
PHST- 2019/04/26 00:00 [received]
PHST- 2019/09/10 00:00 [accepted]
PHST- 2019/11/28 06:00 [entrez]
PHST- 2019/11/28 06:00 [pubmed]
PHST- 2020/04/11 06:00 [medline]
PHST- 2019/10/20 00:00 [pmc-release]
AID - 10.1155/2019/5316984 [doi]
PST - epublish
SO  - Oxid Med Cell Longev. 2019 Oct 20;2019:5316984. doi: 10.1155/2019/5316984. 
      eCollection 2019.