PMID- 31773707
OWN - NLM
STAT- MEDLINE
DCOM- 20201214
LR  - 20201214
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 23
IP  - 21
DP  - 2019 Nov
TI  - Effects of lncRNA MALAT1-mediated beta-catenin signaling pathway on myocardial cell 
      apoptosis in rats with myocardial ischemia/reperfusion injury.
PG  - 9557-9565
LID - 19450 [pii]
LID - 10.26355/eurrev_201911_19450 [doi]
AB  - OBJECTIVE: To investigate the effects of long non-coding ribonucleic acid 
      (lncRNA) metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) on 
      myocardial ischemia/reperfusion (I/R) injury in rats and its mechanism, and to 
      provide a certain reference for the clinical prevention and treatment of 
      myocardial infarction. MATERIALS AND METHODS: A total of 60 male Sprague-Dawley 
      rats were divided into 3 groups using a random number table, including the Sham 
      group (n=20), I/R group (n=20) and I/R + MALAT1 small interfering RNA (siRNA) 
      group (n=20). An I/R model was established by means of recanalization after 
      ligation of the left anterior descending coronary artery of the rats. The rats in 
      the I/R + MALAT1 siRNA group were used to establish a model of MALAT1 knockdown 
      by injecting MALAT1 siRNA from the tail vein. The myocardial infarction area in 
      each group was detected via 2,3,5-triphenyl tetrazolium chloride (TTC) staining. 
      The ejection fraction% (EF%) and fractional shortening% (FS%) of the heart in 
      each group were measured through echocardiography. Hematoxylin and eosin (H&E) 
      staining was adopted to determine the morphological changes in myocardial cells 
      in each group. Terminal deoxynucleotidyl transferase-mediated dUTP nick end 
      labeling (TUNEL) staining was performed to detect the apoptosis levels of 
      myocardial cells and fibroblasts in the cardiac tissues in each group, and 
      Western blotting assay was conducted to measure the expression levels of 
      apoptosis-related proteins [B-cell lymphoma-2 (Bcl-2) and Bcl-2-associated X 
      protein (Bax)]. In addition, the content of beta-catenin in the three groups of rats 
      was determined via immunohistochemical staining. Finally, the impacts of MALAT1 
      siRNA on the expression level of beta-catenin protein were detected using Western 
      blotting assay. RESULTS: MALAT1 siRNA could prominently ameliorate the 
      I/R-induced cardiac insufficiency in the rats and improve the EF% and FS% of the 
      heart (p<0.05). Moreover, MALAT1 siRNA was able to remarkably inhibit the I/R 
      injury-induced myocardial infarction, reducing the infarction area from 
      (59.54+/-3.45) to (24.85+/-1.30; p<0.05). The results of the H&E staining indicated 
      that compared with those in the I/R group, the myofilaments of the myocardial 
      cells were well-arranged, the degrees of degradation and necrosis of the 
      myofilaments declined, and the cellular edema was relieved markedly in the I/R + 
      MALAT1 siRNA group. It was shown in the results of immunohistochemistry and 
      Western blotting that MALAT1 siRNA could notably reverse the I/R-induced 
      up-regulation of beta-catenin expression (p<0.05). CONCLUSIONS: MALAT1 knockdown can 
      significantly ameliorate the I/R-induced myocardial injury and improve the 
      cardiac function of the rats, whose mechanism is probably correlated with the 
      inhibition of MALAT1 siRNA on beta-catenin. Therefore, MALAT1 siRNA is expected to 
      become a new target for the treatment of myocardial infarction.
FAU - Xu, X-Z
AU  - Xu XZ
AD  - Department of Cardiovascular Medicine, Affiliated Langdong Hospital of Guangxi 
      Medical University, Nanning, China. Zhengwenqing369@163.com.
FAU - Luo, B
AU  - Luo B
FAU - Xiao, Y
AU  - Xiao Y
FAU - Zheng, W-Q
AU  - Zheng WQ
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
RN  - 0 (MALAT1 long noncoding RNA, rat)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (beta Catenin)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Disease Models, Animal
MH  - Male
MH  - Myocardial Reperfusion Injury/*metabolism/pathology
MH  - Myocytes, Cardiac/metabolism/pathology
MH  - RNA, Long Noncoding/genetics/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Signal Transduction
MH  - beta Catenin/metabolism
EDAT- 2019/11/28 06:00
MHDA- 2020/12/15 06:00
CRDT- 2019/11/28 06:00
PHST- 2019/11/28 06:00 [entrez]
PHST- 2019/11/28 06:00 [pubmed]
PHST- 2020/12/15 06:00 [medline]
AID - 19450 [pii]
AID - 10.26355/eurrev_201911_19450 [doi]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2019 Nov;23(21):9557-9565. doi: 
      10.26355/eurrev_201911_19450.