PMID- 31776587 OWN - NLM STAT- MEDLINE DCOM- 20200512 LR - 20200518 IS - 1432-072X (Electronic) IS - 0302-8933 (Linking) VI - 202 IP - 3 DP - 2020 Apr TI - Lysobacter terrigena sp. nov., isolated from a Korean soil sample. PG - 637-643 LID - 10.1007/s00203-019-01776-z [doi] AB - A bacterial strain isolated from a soil collected in Jeju Island, designated as 17J7-1(T), was Gram-negative, rod-shaped, yellow colored, and motile by gliding. This strain was able to grow at temperature range from 10 to 42 degrees C, pH 7-9, and tolerated up to 1% NaCl. Analysis of 16S rRNA sequence identified strain 17J7-1(T) as a member of the genus Lysobacter with close sequence similarity with Lysobacter mobilis 9NM-14(T) (97.4%), Lysobacter xinjiangensis RCML-52(T) (97.0%), and Lysobacter humi FJY8(T) (96.9%). The genomic DNA G + C content of the isolate was 67.9 mol%. DNA-DNA relatedness between strain 17J7-1(T) and L. mobilis, L. humi, and L. xinjiangensis were 42.3%, 39.5%, and 35.8%, respectively, clearly showing that the isolate is distinct from its closest phylogenetic neighbors in the genus Lysobacter. Average nucleotide identity (ANI) and digital DNA-DNAhybridization (dDDH) values between strain 17J7-1(T) and L. enzymogenes ATCC 29487(T), the type species of this genus, and several other close Lysobacter species were less than 77% and 22%, respectively. Major fatty acids were C(16:0) iso (29.8%), summed feature 9 (C(17:1) iso omega9c/C(16:0) 10-methyl; 20.1%), and C(15:0) iso (17.7%). The predominant respiratory quinone was ubiquinone Q-8 and the major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol. In the light of the polyphasic evidence accumulated in this study, strain 17J7-1(T) is considered to represent a novel species in the genus Lysobacter, for which name Lysobacter terrigena sp. nov. is proposed. The type strain is 17J7-1(T) (= KCTC 62217(T) = JCM 33057(T)). FAU - Li, Weilan AU - Li W AD - School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea. FAU - Elderiny, Nabil Salah AU - Elderiny NS AD - School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea. FAU - Ten, Leonid N AU - Ten LN AD - School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea. FAU - Lee, Seung-Yeol AU - Lee SY AD - School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea. FAU - Kim, Myung Kyum AU - Kim MK AD - Department of Bio and Environmental Technology, Seoul Women's University, Seoul, 01797, Republic of Korea. FAU - Jung, Hee-Young AU - Jung HY AD - School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea. heeyoung@knu.ac.kr. AD - Institute of Plant Medicine, Kyungpook National University, Daegu, 41566, Republic of Korea. heeyoung@knu.ac.kr. LA - eng GR - 2018H1D3A2065415/National Research Foundation of Korea (KR)/ PT - Journal Article DEP - 20191127 PL - Germany TA - Arch Microbiol JT - Archives of microbiology JID - 0410427 RN - 0 (DNA, Bacterial) RN - 0 (Fatty Acids) RN - 0 (Phospholipids) RN - 0 (RNA, Ribosomal, 16S) SB - IM MH - Bacterial Typing Techniques MH - Base Composition MH - DNA, Bacterial/genetics MH - Fatty Acids/analysis/metabolism MH - Lysobacter/classification/genetics/*isolation & purification/metabolism MH - Phospholipids/analysis/metabolism MH - Phylogeny MH - RNA, Ribosomal, 16S/genetics MH - Republic of Korea MH - Sequence Analysis, DNA MH - *Soil Microbiology OTO - NOTNLM OT - Lysobacter OT - Lysobacteraceae OT - Soil bacteria EDAT- 2019/11/30 06:00 MHDA- 2020/05/19 06:00 CRDT- 2019/11/29 06:00 PHST- 2018/12/11 00:00 [received] PHST- 2019/11/18 00:00 [accepted] PHST- 2019/05/08 00:00 [revised] PHST- 2019/11/30 06:00 [pubmed] PHST- 2020/05/19 06:00 [medline] PHST- 2019/11/29 06:00 [entrez] AID - 10.1007/s00203-019-01776-z [pii] AID - 10.1007/s00203-019-01776-z [doi] PST - ppublish SO - Arch Microbiol. 2020 Apr;202(3):637-643. doi: 10.1007/s00203-019-01776-z. Epub 2019 Nov 27.