PMID- 31777226
OWN - NLM
STAT- MEDLINE
DCOM- 20191203
LR  - 20200108
IS  - 1000-0607 (Print)
IS  - 1000-0607 (Linking)
VI  - 44
IP  - 11
DP  - 2019 Nov 25
TI  - [Effect of different courses of electroacupuncture intervention on recognition 
      memory and proliferation and differentiation of hippocampal neural stem cells in 
      mice with radiation-induced brain injury].
PG  - 787-92
LID - 10.13702/j.1000-0607.190017 [doi]
AB  - OBJECTIVE: To observe the influence of different courses of electroacupuncture 
      (EA) intervention on recognition memory and the proliferation and differentiation 
      of hippocampal neural stem cells in mice with radiation-induced brain injury, so 
      as to explore its mechanisms underlying improving radiation-induced brain injury. 
      METHODS: Se-venty 30-day old C57BL/6J mice were randomly divided into control, 
      model and EA groups, and the latter two groups were further divided into 1 week 
      (W), 2 W and 3 W subgroups (n=10 in the control group and each subgroup). The 
      ra-diation-induced brain injury model was established by radiating the mouse' 
      left head at a dose of 8 Gy for 10 min by using a radiation linear accelerator. 
      EA (1.5 V, 2 Hz/10 Hz) was applied to "Baihui" (GV20), "Fengfu" (GV14) and 
      bilateral "Shenshu" (BL23) for 30 min, once daily for 1, 2 and 3 weeks, 
      respectively. The learning-cognition memory ability was detected by using novel 
      object recognition test in an open test box to record the time for exploring a 
      novel object (TN) and a familiar object and to calculate the recognition index 
      (RI). The neural stem cells' proliferation and differentiation in the hippocampus 
      tissues were evaluated by counting the number of bromodeoxyuridine (BrdU)-labeled 
      cells, neuronal nuclei (NeuN)/BrdU-positive cells and BrdU/glia fibrillary acidic 
      protein (GFAP)-positive cells under microscope after immunofluorescence stain. 
      RESULTS: After modeling, the TN at 90 min and 24 h and RI of the model subgroup 3 
      W at 90 min and RI of the model subgroup 1, 2 and 3 W at 24 h were significantly 
      decreased in comparison with those of the control group (P<0.01, P<0.05). 
      Moreover, the number of BrdU-positive cells in the model subgroup 1 W and 2 W, 
      the BrdU/NeuN double-labeled cells in the 3 model subgroups and BrdU/GFAP 
      double-labeled cells in the model subgroup 1 W and 3 W were significantly 
      decreased (P<0.01, P<0.05). Following EA interventions, the TN in the 3 EA 
      subgroups at both 90 min and 24 h, and RI of EA subgroup 3 W at 90 min and EA 
      subgroup 2 W and 3 W at 24 h were considerably increased compared with those of 
      the corresponding 3 model subgroups (P<0.05, P<0.01). The numbers of 
      BrdU-positive cells as well as BrdU/NeuN and BrdU/GFAP double-labeled cells were 
      significantly increased in the 3 EA subgroups (P<0.05, P<0.01, P<0.001). 
      CONCLUSION: EA of GV20, GV14 and BL23 can improve the recognition memory ability 
      of mice with radiation-induced brain injury, which may be related to its effect 
      in promoting the proliferation and differentiation of stem cells in the 
      hippocampus.
FAU - Wu, Xin
AU  - Wu X
AD  - School of Basic Medicine, Henan University of Chinese Medicine, Zhengzhou 450046, 
      China.
FAU - Sun, Ning-Ning
AU  - Sun NN
AD  - School of Basic Medicine, Henan University of Chinese Medicine, Zhengzhou 450046, 
      China.
FAU - Lu, Ming-Hui
AU  - Lu MH
AD  - School of Basic Medicine, Henan University of Chinese Medicine, Zhengzhou 450046, 
      China.
FAU - Su, Shao-Hua
AU  - Su SH
AD  - School of Basic Medicine, Henan University of Chinese Medicine, Zhengzhou 450046, 
      China.
FAU - Wang, Dong-Hui
AU  - Wang DH
AD  - School of Basic Medicine, Henan University of Chinese Medicine, Zhengzhou 450046, 
      China.
FAU - Zhang, Song-Jiang
AU  - Zhang SJ
AD  - School of Basic Medicine, Henan University of Chinese Medicine, Zhengzhou 450046, 
      China.
FAU - Gao, Jian-Feng
AU  - Gao JF
AD  - School of Basic Medicine, Henan University of Chinese Medicine, Zhengzhou 450046, 
      China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhen Ci Yan Jiu
JT  - Zhen ci yan jiu = Acupuncture research
JID - 8507710
SB  - IM
MH  - Animals
MH  - *Brain Injuries
MH  - Cell Differentiation
MH  - Cell Proliferation
MH  - *Electroacupuncture
MH  - Hippocampus
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - *Neural Stem Cells
OTO - NOTNLM
OT  - Electroacupuncture
OT  - Hippocampus
OT  - Neural stem cell
OT  - Proli-feration and differentiation
OT  - Radiation-induced brain injury
OT  - Recognition memory
EDAT- 2019/11/30 06:00
MHDA- 2019/12/04 06:00
CRDT- 2019/11/29 06:00
PHST- 2019/11/29 06:00 [entrez]
PHST- 2019/11/30 06:00 [pubmed]
PHST- 2019/12/04 06:00 [medline]
AID - 10.13702/j.1000-0607.190017 [doi]
PST - ppublish
SO  - Zhen Ci Yan Jiu. 2019 Nov 25;44(11):787-92. doi: 10.13702/j.1000-0607.190017.