PMID- 31779264
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 1996-1944 (Print)
IS  - 1996-1944 (Electronic)
IS  - 1996-1944 (Linking)
VI  - 12
IP  - 23
DP  - 2019 Nov 26
TI  - Use of Genetically Modified Bacteria to Repair Cracks in Concrete.
LID - 10.3390/ma12233912 [doi]
LID - 3912
AB  - In this paper, we studied the crack-repair by spraying bacteria-based liquid 
      around the cracks in concrete. To enhance the repair efficiency and speed up the 
      repair process, the transposon mutagenesis method was employed to modify the 
      genes of Bacillus halodurans and create a mutant bacterial strain with higher 
      efficiency of calcium carbonate productivity by catalyzing the combination of 
      carbonate and calcium ion. The efficiency of crack-repairing in concrete by 
      spraying two kinds of bacterial liquid was evaluated via image analysis, X-ray 
      computed tomography (X-CT) scanning technology and the sorptivity test. The 
      results show that the crack-repair efficiency was enhanced very evidently by 
      spraying genetically modified bacterial-liquid as no microbiologically induced 
      calcite precipitation (MICP) was found within the cracks for concrete samples 
      sprayed using wild type bacterial-liquid. In addition, the crack-repair process 
      was also shortened significantly in the case of genetically modified bacteria.
FAU - Zhang, Zhigang
AU  - Zhang Z
AD  - Key Laboratory of New Technology for Construction of Cities in Mountain Area, 
      Chongqing University, Ministry of Education, Chongqing 400045, China.
AD  - School of Civil and Environmental Engineering, Nanyang Technological University, 
      Singapore 637551, Singapore.
FAU - Weng, Yiwei
AU  - Weng Y
AD  - School of Civil and Environmental Engineering, Nanyang Technological University, 
      Singapore 637551, Singapore.
AD  - Singapore Centre for 3D Printing, School of Mechanical and Aerospace Engineering, 
      Singapore 639798, Singapore.
FAU - Ding, Yuanzhao
AU  - Ding Y
AD  - School of Civil and Environmental Engineering, Nanyang Technological University, 
      Singapore 637551, Singapore.
FAU - Qian, Shunzhi
AU  - Qian S
AD  - School of Civil and Environmental Engineering, Nanyang Technological University, 
      Singapore 637551, Singapore.
LA  - eng
GR  - 51708061/National Natural Science Foundation of China/
GR  - 111 Project of China (Grant No. B18062)/Ministry of Education of the People's 
      Republic of China/
GR  - 2019CDJSK04XK23/the State Education Ministry and Fundamental Research Funds for 
      the Central Universities/
PT  - Journal Article
DEP - 20191126
PL  - Switzerland
TA  - Materials (Basel)
JT  - Materials (Basel, Switzerland)
JID - 101555929
PMC - PMC6926745
OTO - NOTNLM
OT  - bacterial technique
OT  - concrete
OT  - genetic modification
OT  - repairing efficiency
COIS- The authors declare no conflict of interest.
EDAT- 2019/11/30 06:00
MHDA- 2019/11/30 06:01
PMCR- 2019/11/26
CRDT- 2019/11/30 06:00
PHST- 2019/10/22 00:00 [received]
PHST- 2019/11/13 00:00 [revised]
PHST- 2019/11/22 00:00 [accepted]
PHST- 2019/11/30 06:00 [entrez]
PHST- 2019/11/30 06:00 [pubmed]
PHST- 2019/11/30 06:01 [medline]
PHST- 2019/11/26 00:00 [pmc-release]
AID - ma12233912 [pii]
AID - materials-12-03912 [pii]
AID - 10.3390/ma12233912 [doi]
PST - epublish
SO  - Materials (Basel). 2019 Nov 26;12(23):3912. doi: 10.3390/ma12233912.