PMID- 31779691
OWN - NLM
STAT- MEDLINE
DCOM- 20200724
LR  - 20200724
IS  - 1757-6512 (Electronic)
IS  - 1757-6512 (Linking)
VI  - 10
IP  - 1
DP  - 2019 Nov 28
TI  - Exposure to blue light stimulates the proangiogenic capability of exosomes 
      derived from human umbilical cord mesenchymal stem cells.
PG  - 358
LID - 10.1186/s13287-019-1472-x [doi]
LID - 358
AB  - BACKGROUND: The therapeutic potential of mesenchymal stem cells (MSCs) may be 
      attributed partly to the secreted paracrine factors, which comprise exosomes. 
      Exosomes are small, saucer-shaped vesicles containing miRNAs, mRNAs, and 
      proteins. Exosomes derived from human umbilical cord mesenchymal stem cells 
      (hUC-MSCs) have been reported to promote angiogenesis. However, the efficacy of 
      exosome-based therapies is still limited both in vitro and in vivo. The present 
      study aimed to develop a new optical manipulation approach to stimulate the 
      proangiogenic potential of exosomes and characterize its mechanism underlying 
      tissue regeneration. METHODS: We used blue (455 nm) and red (638 nm) 
      monochromatic light exposure to investigate the processing of stimuli. Exosomes 
      were prepared by QIAGEN exoEasy Maxi kit and confirmed to be present by 
      transmission electron microscopy and immunoblotting analyses. The proangiogenic 
      activity of blue light-treated human umbilical vein endothelial cells (HUVECs), 
      when co-cultured with hUC-MSCs, was assessed by EdU (5-ethynyl-2'-deoxyuridine) 
      incorporation, wound closure, and endothelial tube formation assays. The in vivo 
      angiogenic activity of blue light-treated MSC-derived exosomes (MSC-Exs) was 
      evaluated using both murine matrigel plug and skin wound models. RESULTS: We 
      found that 455-nm blue light is effective for promoting proliferation, migration, 
      and tube formation of HUVECs co-cultured with MSCs. Furthermore, MSC-Exs 
      stimulated in vivo angiogenesis and their proangiogenic potential were enhanced 
      significantly upon blue light illumination. Finally, activation of the 
      endothelial cells in response to stimulation by blue light-treated exosomes was 
      demonstrated by upregulation of two miRNAs, miR-135b-5p, and miR-499a-3p. 
      CONCLUSIONS: Blue (455 nm) light illumination improved the therapeutic effects of 
      hUC-MSC exosomes by enhancing their proangiogenic ability in vitro and in vivo 
      with the upregulation of the following two miRNAs: miR-135b-5p and miR-499a-3p.
FAU - Yang, Kun
AU  - Yang K
AD  - Key Laboratory of Experimental Teratology, Ministry of Education Department of 
      Medical Genetics, School of Basic Medical Sciences, Shandong University, 44 Wen 
      Hua Xi Road, Jinan, Shandong, 250012, People's Republic of China.
FAU - Li, Dong
AU  - Li D
AD  - Cryomedicine Laboratory, Qilu Hospital of Shandong University, Jinan, 250012, 
      Shandong, China.
AD  - Stem Cell and Regenerative Medicine Research Center of Shandong University, 
      Jinan, 250012, Shandong, China.
FAU - Wang, Meitian
AU  - Wang M
AD  - Key Laboratory of Experimental Teratology, Ministry of Education Department of 
      Medical Genetics, School of Basic Medical Sciences, Shandong University, 44 Wen 
      Hua Xi Road, Jinan, Shandong, 250012, People's Republic of China.
FAU - Xu, Zhiliang
AU  - Xu Z
AD  - Key Laboratory of Experimental Teratology, Ministry of Education Department of 
      Medical Genetics, School of Basic Medical Sciences, Shandong University, 44 Wen 
      Hua Xi Road, Jinan, Shandong, 250012, People's Republic of China.
FAU - Chen, Xiao
AU  - Chen X
AD  - Key Laboratory of Experimental Teratology, Ministry of Education Department of 
      Medical Genetics, School of Basic Medical Sciences, Shandong University, 44 Wen 
      Hua Xi Road, Jinan, Shandong, 250012, People's Republic of China.
FAU - Liu, Qiao
AU  - Liu Q
AD  - Key Laboratory of Experimental Teratology, Ministry of Education Department of 
      Medical Genetics, School of Basic Medical Sciences, Shandong University, 44 Wen 
      Hua Xi Road, Jinan, Shandong, 250012, People's Republic of China.
FAU - Sun, Wenjie
AU  - Sun W
AD  - Key Laboratory of Experimental Teratology, Ministry of Education Department of 
      Medical Genetics, School of Basic Medical Sciences, Shandong University, 44 Wen 
      Hua Xi Road, Jinan, Shandong, 250012, People's Republic of China.
FAU - Li, Jiangxia
AU  - Li J
AD  - Key Laboratory of Experimental Teratology, Ministry of Education Department of 
      Medical Genetics, School of Basic Medical Sciences, Shandong University, 44 Wen 
      Hua Xi Road, Jinan, Shandong, 250012, People's Republic of China.
FAU - Gong, Yaoqin
AU  - Gong Y
AD  - Key Laboratory of Experimental Teratology, Ministry of Education Department of 
      Medical Genetics, School of Basic Medical Sciences, Shandong University, 44 Wen 
      Hua Xi Road, Jinan, Shandong, 250012, People's Republic of China.
FAU - Liu, Duo
AU  - Liu D
AD  - State Key Laboratory of Crystal Materials, Shandong University, Jinan, Shandong, 
      250100, People's Republic of China.
FAU - Shao, Changshun
AU  - Shao C
AD  - The First Affiliated Hospital of Soochow University and State Key Laboratory of 
      Radiation Medicine and Protection, Institutes for Translational Medicine, Soochow 
      University, Suzhou, 215123, Jiangsu, China.
FAU - Liu, Qiji
AU  - Liu Q
AD  - Key Laboratory of Experimental Teratology, Ministry of Education Department of 
      Medical Genetics, School of Basic Medical Sciences, Shandong University, 44 Wen 
      Hua Xi Road, Jinan, Shandong, 250012, People's Republic of China.
FAU - Li, Xi
AU  - Li X
AUID- ORCID: 0000-0003-2912-5803
AD  - Key Laboratory of Experimental Teratology, Ministry of Education Department of 
      Medical Genetics, School of Basic Medical Sciences, Shandong University, 44 Wen 
      Hua Xi Road, Jinan, Shandong, 250012, People's Republic of China. 
      lixi@sdu.edu.cn.
AD  - Advanced Medical Research Institute, Shandong University, Jinan, 250012, 
      Shandong, China. lixi@sdu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191128
PL  - England
TA  - Stem Cell Res Ther
JT  - Stem cell research & therapy
JID - 101527581
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Animals
MH  - Burns/pathology/therapy
MH  - Cell Movement/radiation effects
MH  - Cell Proliferation/radiation effects
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Disease Models, Animal
MH  - Exosomes/metabolism/*radiation effects/transplantation
MH  - Human Umbilical Vein Endothelial Cells
MH  - Humans
MH  - *Light
MH  - Male
MH  - Mesenchymal Stem Cells/cytology/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - MicroRNAs/metabolism
MH  - Neovascularization, Physiologic/*radiation effects
MH  - Umbilical Cord/cytology
MH  - Up-Regulation/radiation effects
PMC - PMC6883639
OTO - NOTNLM
OT  - Angiogenesis
OT  - Exosomes
OT  - Light exposure
OT  - Mesenchymal stem cells
OT  - microRNAs
COIS- The authors declare that they have no competing interests.
EDAT- 2019/11/30 06:00
MHDA- 2020/07/25 06:00
PMCR- 2019/11/28
CRDT- 2019/11/30 06:00
PHST- 2019/07/11 00:00 [received]
PHST- 2019/10/28 00:00 [accepted]
PHST- 2019/09/20 00:00 [revised]
PHST- 2019/11/30 06:00 [entrez]
PHST- 2019/11/30 06:00 [pubmed]
PHST- 2020/07/25 06:00 [medline]
PHST- 2019/11/28 00:00 [pmc-release]
AID - 10.1186/s13287-019-1472-x [pii]
AID - 1472 [pii]
AID - 10.1186/s13287-019-1472-x [doi]
PST - epublish
SO  - Stem Cell Res Ther. 2019 Nov 28;10(1):358. doi: 10.1186/s13287-019-1472-x.