PMID- 31781660
OWN - NLM
STAT- MEDLINE
DCOM- 20200427
LR  - 20220411
IS  - 2314-6753 (Electronic)
IS  - 2314-6745 (Print)
VI  - 2019
DP  - 2019
TI  - Effects of Negative Pressure Wound Therapy on Levels of Angiopoetin-2 and Other 
      Selected Circulating Signaling Molecules in Patients with Diabetic Foot Ulcer.
PG  - 1756798
LID - 10.1155/2019/1756798 [doi]
LID - 1756798
AB  - BACKGROUND AND AIMS: Diabetic foot ulcers (DFUs) are linked to amputations and 
      premature deaths. Negative pressure wound therapy (NPWT) has been used for DFUs. 
      The mechanism of NPWT's action may be associated with its influence on 
      circulating molecules. We assessed NPWT's effect on the plasma levels of 
      angiopoietin-2 (Ang2), a key regulator of angiogenesis, and its microvesicular 
      receptors (Tie2) as well as the microvesicles (MVs) themselves in DFU patients. 
      MATERIALS AND METHODS: We included 69 patients with type 2 diabetes mellitus 
      (T2DM) and neuropathic, noninfected DFUs-49 were treated with NPWT and 20 were 
      treated with standard therapy (ST). Assigning patients to the NPWT group was not 
      random but based on DFU characteristics, especially wound area. Ang2 was measured 
      by ELISA in the entire group, while in a subgroup of 19 individuals on NPWT and 
      10 on ST, flow cytometry was used to measure Tie2+ and the corresponding isotype 
      control (Iso+) and annexin V (AnnV+) as well as total MVs. Measurements were 
      performed at the beginning and after 8 +/- 1 days of therapy. RESULTS: Treatment 
      groups were similar for basic characteristics but differed by their median DFU 
      areas (10.3 (4.2-18.9) vs. 1.3 (0.9-3.4) cm(2), p = 0.0001). At day 0, no 
      difference was observed in Ang2 levels, total MVs, MV Tie+, and MV AnnV+ between 
      the groups. Ang2 decreased after 8 days in the NPWT group, unlike in the ST group 
      (3.54 (2.40-5.40) vs. 3.32 (2.33-4.61), p = 0.02, and 3.19 +/- 1.11 vs. 3.19 +/- 
      1.29 ng/mL, p = 0.98, respectively). No other parameters were identified that may 
      have been influenced by the NPWT treatment. CONCLUSION: NPWT in T2DM patients 
      with neuropathic, noninfected DFU seems to lead to reduction of the Ang2 level. 
      Influencing the level of Ang2 may constitute one of NPWT-related mechanisms to 
      accelerate wound healing.
CI  - Copyright (c) 2019 Jerzy Hohendorff et al.
FAU - Hohendorff, Jerzy
AU  - Hohendorff J
AD  - Department of Metabolic Diseases, Jagiellonian University Medical College, 
      Krakow, Poland.
AD  - Clinic of Metabolic Diseases, University Hospital, Krakow, Poland.
FAU - Drozdz, Anna
AU  - Drozdz A
AUID- ORCID: 0000-0003-3999-6725
AD  - Department of Medical Physics, Marian Smoluchowski Institute of Physics, 
      Jagiellonian University, Krakow, Poland.
AD  - Malopolska Center of Biotechnology, Jagiellonian University, Krakow, Poland.
FAU - Borys, Sebastian
AU  - Borys S
AD  - Department of Metabolic Diseases, Jagiellonian University Medical College, 
      Krakow, Poland.
AD  - Clinic of Metabolic Diseases, University Hospital, Krakow, Poland.
FAU - Ludwig-Slomczynska, Agnieszka H
AU  - Ludwig-Slomczynska AH
AD  - Center for Medical Genomics OMICRON, Jagiellonian University Medical College, 
      Krakow, Poland.
FAU - Kiec-Wilk, Beata
AU  - Kiec-Wilk B
AD  - Department of Metabolic Diseases, Jagiellonian University Medical College, 
      Krakow, Poland.
AD  - Clinic of Metabolic Diseases, University Hospital, Krakow, Poland.
FAU - Stepien, Ewa L
AU  - Stepien EL
AUID- ORCID: 0000-0003-3589-1715
AD  - Department of Medical Physics, Marian Smoluchowski Institute of Physics, 
      Jagiellonian University, Krakow, Poland.
FAU - Malecki, Maciej T
AU  - Malecki MT
AUID- ORCID: 0000-0002-4794-0820
AD  - Department of Metabolic Diseases, Jagiellonian University Medical College, 
      Krakow, Poland.
AD  - Clinic of Metabolic Diseases, University Hospital, Krakow, Poland.
LA  - eng
PT  - Journal Article
DEP - 20191028
PL  - England
TA  - J Diabetes Res
JT  - Journal of diabetes research
JID - 101605237
RN  - 0 (ANGPT2 protein, human)
RN  - 0 (Angiopoietin-2)
RN  - 0 (Biomarkers)
RN  - EC 2.7.10.1 (Receptor, TIE-2)
RN  - EC 2.7.10.1 (TEK protein, human)
SB  - IM
MH  - Aged
MH  - Angiopoietin-2/*blood
MH  - Biomarkers/blood
MH  - Diabetic Foot/blood/diagnosis/*therapy
MH  - Female
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - *Negative-Pressure Wound Therapy/adverse effects
MH  - Neovascularization, Physiologic
MH  - Pilot Projects
MH  - Receptor, TIE-2/blood
MH  - Time Factors
MH  - Treatment Outcome
MH  - *Wound Healing
PMC - PMC6855047
COIS- The authors declare no conflicts of interest.
EDAT- 2019/11/30 06:00
MHDA- 2020/04/28 06:00
PMCR- 2019/10/28
CRDT- 2019/11/30 06:00
PHST- 2019/07/12 00:00 [received]
PHST- 2019/09/26 00:00 [accepted]
PHST- 2019/11/30 06:00 [entrez]
PHST- 2019/11/30 06:00 [pubmed]
PHST- 2020/04/28 06:00 [medline]
PHST- 2019/10/28 00:00 [pmc-release]
AID - 10.1155/2019/1756798 [doi]
PST - epublish
SO  - J Diabetes Res. 2019 Oct 28;2019:1756798. doi: 10.1155/2019/1756798. eCollection 
      2019.