PMID- 31782420
OWN - NLM
STAT- MEDLINE
DCOM- 20200928
LR  - 20200928
IS  - 1364-5528 (Electronic)
IS  - 0003-2654 (Linking)
VI  - 145
IP  - 2
DP  - 2020 Jan 20
TI  - Study on the biochemical mechanisms of the micro-wave ablation treatment of lung 
      cancer by ex vivo confocal Raman microspectral imaging.
PG  - 626-635
LID - 10.1039/c9an01524h [doi]
AB  - As a highly invasive and the most prevalent malignancy, lung cancer remains the 
      leading cause of cancer-associated mortality worldwide, especially in China. 
      Microwave ablation (MWA) is an effective, safe, and the least invasive ablative 
      treatment modality, which has been increasingly used for the management of 
      unrespectable lung tumors. However, the underlying biochemical mechanisms of MWA 
      treatment remain to be incompletely elucidated. Therefore, to illustrate the 
      complex biochemical responses of lung squamous cell carcinoma (LSCC) to MWA 
      treatment, confocal Raman micro-spectral imaging (CRMI) was applied in 
      combination with multivariate analysis. A total of twelve LSCC tissues were 
      acquired from patients undergoing clinical treatment, and their spectral 
      characteristics were analyzed to determine significant spectral variations 
      following cancer progression and MWA treatment in comparison with healthy lung 
      tissues. Point-scanned Raman datasets were acquired from sectioned tissue samples 
      in both pre-therapy (Pre-MWA group) and post-therapy groups (Post-MWA group) and 
      further analyzed using K-means cluster analysis (KCA) and principal component 
      analysis (PCA) to highlight the detailed compositional variations of the 
      biochemical constituents. The spectral variations of essential amino acids (such 
      as phenylalanine and tryptophan), collagen, and nucleic acids in the cancerous 
      tissues of the Post-MWA group were significantly enhanced compared to those in 
      the Pre-MWA group. The acquired information further confirmed a remarkable 
      increase in the content of nucleic acid, protein, and lipid in the cancerous 
      tissue following MWA treatment and, a comparative spectral imaging investigation 
      indicated that MWA had no noticeable adverse effects on the paracancerous 
      tissues. Thus, the findings not only illustrated the underlying biochemical 
      variability in lung cancer during MWA treatment but also further confirmed the 
      feasibility of a combined analytical procedure for assessing the biochemical 
      responses during thermal ablation, which could be applied to prominently enhance 
      the effectiveness of MWA in lung cancer treatment in clinical settings.
FAU - Song, Dongliang
AU  - Song D
AD  - Institute of Photonics and Photon-Technology, Northwest University, Xi'an, 
      Shaanxi 710069, China. swang@nwu.edu.cn.
FAU - Chen, Tianming
AU  - Chen T
FAU - Wang, Shuang
AU  - Wang S
FAU - Chen, Shilin
AU  - Chen S
FAU - Li, Heping
AU  - Li H
FAU - Yu, Fan
AU  - Yu F
FAU - Zhang, Jingyuan
AU  - Zhang J
FAU - Zhang, Zhe
AU  - Zhang Z
LA  - eng
PT  - Journal Article
PL  - England
TA  - Analyst
JT  - The Analyst
JID - 0372652
SB  - IM
MH  - Carcinoma, Non-Small-Cell Lung/*pathology/therapy
MH  - Case-Control Studies
MH  - Catheter Ablation/*methods
MH  - Cluster Analysis
MH  - Humans
MH  - Lung Neoplasms/*pathology/therapy
MH  - Male
MH  - Microwaves/*therapeutic use
MH  - Molecular Imaging/*methods
MH  - Principal Component Analysis
MH  - Spectrum Analysis, Raman/*methods
EDAT- 2019/11/30 06:00
MHDA- 2020/09/29 06:00
CRDT- 2019/11/30 06:00
PHST- 2019/11/30 06:00 [pubmed]
PHST- 2020/09/29 06:00 [medline]
PHST- 2019/11/30 06:00 [entrez]
AID - 10.1039/c9an01524h [doi]
PST - ppublish
SO  - Analyst. 2020 Jan 20;145(2):626-635. doi: 10.1039/c9an01524h.