PMID- 31783860
OWN - NLM
STAT- MEDLINE
DCOM- 20200504
LR  - 20200505
IS  - 1477-7827 (Electronic)
IS  - 1477-7827 (Linking)
VI  - 17
IP  - 1
DP  - 2019 Nov 29
TI  - Analysis of key genes and their functions in placental tissue of patients with 
      gestational diabetes mellitus.
PG  - 104
LID - 10.1186/s12958-019-0546-z [doi]
LID - 104
AB  - BACKGROUND: This study was aimed at screening out the potential key genes and 
      pathways associated with gestational diabetes mellitus (GDM). METHODS: The 
      GSE70493 dataset used for this study was obtained from the Gene Expression 
      Omnibus database. Differentially expressed genes (DEGs) in the placental tissue 
      of women with GDM in relation to the control tissue samples were identified and 
      submitted to protein-protein interaction (PPI) network analysis and subnetwork 
      module mining. Functional enrichment analyses of the PPI network and subnetworks 
      were subsequently carried out. Finally, the integrated miRNA-transcription factor 
      (TF)-DEG regulatory network was analyzed. RESULTS: In total, 238 DEGs were 
      identified, of which 162 were upregulated and 76 were downregulated. Through PPI 
      network construction, 108 nodes and 278 gene pairs were obtained, from which 
      chemokine (C-X-C motif) ligand 9 (CXCL9), CXCL10, protein tyrosine phosphatase, 
      receptor type C (PTPRC), and human leukocyte antigen (HLA) were screened out as 
      hub genes. Moreover, genes associated with the immune-related pathway and immune 
      responses were found to be significantly enriched in the process of GDM. Finally, 
      miRNAs and TFs that target the DEGs were predicted. CONCLUSIONS: Four candidate 
      genes (viz., CXCL9, CXCL10, PTPRC, and HLA) are closely related to GDM. 
      miR-223-3p, miR-520, and thioredoxin-binding protein may play important roles in 
      the pathogenesis of this disease.
FAU - Wang, Yuxia
AU  - Wang Y
AD  - Department of Gynecology, Jinan Central Hospital, Jinan City, 250013, Shandong 
      Province, China.
FAU - Yu, Haifeng
AU  - Yu H
AD  - Department of Obstetrics, Jinan Central Hospital, No. 105 Jiefang Road, Lixia 
      District, Jinan City, 250013, Shandong Province, China.
FAU - Liu, Fangmei
AU  - Liu F
AD  - Department of Obstetrics, Jinan Central Hospital, No. 105 Jiefang Road, Lixia 
      District, Jinan City, 250013, Shandong Province, China.
FAU - Song, Xiue
AU  - Song X
AD  - Department of Obstetrics, Jinan Central Hospital, No. 105 Jiefang Road, Lixia 
      District, Jinan City, 250013, Shandong Province, China. xiuesss19@163.com.
LA  - eng
PT  - Journal Article
DEP - 20191129
PL  - England
TA  - Reprod Biol Endocrinol
JT  - Reproductive biology and endocrinology : RB&E
JID - 101153627
RN  - 0 (Carrier Proteins)
RN  - 0 (Chemokine CXCL10)
RN  - 0 (Chemokine CXCL9)
RN  - 0 (HLA Antigens)
RN  - 0 (MicroRNAs)
RN  - 0 (TXNIP protein, human)
RN  - EC 3.1.3.48 (Leukocyte Common Antigens)
RN  - EC 3.1.3.48 (PTPRC protein, human)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatases)
SB  - IM
MH  - Carrier Proteins/genetics/metabolism
MH  - Chemokine CXCL10/genetics/metabolism
MH  - Chemokine CXCL9/genetics/metabolism
MH  - Diabetes, Gestational/*genetics/metabolism
MH  - Female
MH  - Gene Expression Profiling/*methods
MH  - *Gene Regulatory Networks
MH  - HLA Antigens/genetics/metabolism
MH  - Humans
MH  - Leukocyte Common Antigens/genetics/metabolism
MH  - MicroRNAs/genetics/metabolism
MH  - Placenta/*metabolism
MH  - Pregnancy
MH  - Protein Interaction Maps/*genetics
MH  - Protein Tyrosine Phosphatases/genetics/metabolism
PMC - PMC6884804
OTO - NOTNLM
OT  - Differentially expressed genes
OT  - Gestational diabetes mellitus
OT  - Integrated regulatory network
OT  - Protein-protein interaction network
OT  - Transcription factors
COIS- The authors declare that they have no competing interests.
EDAT- 2019/12/01 06:00
MHDA- 2020/05/06 06:00
PMCR- 2019/11/29
CRDT- 2019/12/01 06:00
PHST- 2019/10/11 00:00 [received]
PHST- 2019/11/20 00:00 [accepted]
PHST- 2019/12/01 06:00 [entrez]
PHST- 2019/12/01 06:00 [pubmed]
PHST- 2020/05/06 06:00 [medline]
PHST- 2019/11/29 00:00 [pmc-release]
AID - 10.1186/s12958-019-0546-z [pii]
AID - 546 [pii]
AID - 10.1186/s12958-019-0546-z [doi]
PST - epublish
SO  - Reprod Biol Endocrinol. 2019 Nov 29;17(1):104. doi: 10.1186/s12958-019-0546-z.