PMID- 31784427
OWN - NLM
STAT- MEDLINE
DCOM- 20210430
LR  - 20210430
IS  - 1708-8267 (Electronic)
IS  - 1081-5589 (Print)
IS  - 1081-5589 (Linking)
VI  - 68
IP  - 1
DP  - 2020 Jan
TI  - Multiple myeloma-derived exosomes inhibit osteoblastic differentiation and 
      improve IL-6 secretion of BMSCs from multiple myeloma.
PG  - 45-51
LID - 10.1136/jim-2019-001010 [doi]
AB  - Bone marrow stromal cells (BMSCs) play a critical role in multiple myeloma (MM) 
      pathogenesis by cell contact, and secretion of cytokines, growth factors and 
      extracellular vesicles. Exosomes are secreted by almost all cell types and are 
      recently reported to mediate local cell-to-cell cross-talk by transferring 
      messenger RNAs, LncRNAs, and proteins. Compelling studies have identified 
      BMSC-derived exosomes induce proliferation, migration, survival, and drug 
      resistance of MM cells. However, whether MM cell-derived exosome also plays a 
      role in function in BMSC remains unclear. Here we investigated the effect of MM 
      cell-derived exosomes on the interleukin (IL)-6 secretion and osteoblastic 
      differentiation capability of BMSC from patients with MM. Furthermore we 
      investigated the IL-6 secretion relative regulation protein APE1 and NF-kB and 
      osteoblastic differentiation protein Runx2 (runt-related gene 2), Osterix and 
      osteocalcin (OCN). Our results showed that MM cell-derived exosomes promoted IL-6 
      secretion and suppressed osteoblastic differentiation and mineralization of 
      BMSCs. Mechanistically, we demonstrated that MM cell-derived exosomes lead to an 
      increase in APE1 and NF-kB and a reduction in Runx2, Osterix and OCN in BMSCs. 
      Taken together, MM cell-derived exosomes induce the secretion of IL-6 and poor 
      osteoblastic differentiation of BMSCs.
CI  - (c) American Federation for Medical Research 2020. Re-use permitted under CC BY-NC. 
      No commercial re-use. Published by BMJ.
FAU - Liu, Zhaoyun
AU  - Liu Z
AUID- ORCID: 0000-0002-2730-1562
AD  - Hematology department, Tianjin Medical University General Hospital, Tianjin, 
      China.
FAU - Liu, Hui
AU  - Liu H
AD  - Hematology department, Tianjin Medical University General Hospital, Tianjin, 
      China.
FAU - Li, Yanqi
AU  - Li Y
AD  - Hematology department, Tianjin Medical University General Hospital, Tianjin, 
      China.
FAU - Shao, Qin
AU  - Shao Q
AD  - Hematology department, Tianjin Medical University General Hospital, Tianjin, 
      China.
FAU - Chen, Jin
AU  - Chen J
AD  - Hematology department, Tianjin Medical University General Hospital, Tianjin, 
      China.
FAU - Song, Jia
AU  - Song J
AD  - Hematology department, Tianjin Medical University General Hospital, Tianjin, 
      China.
FAU - Fu, Rong
AU  - Fu R
AD  - Hematology department, Tianjin Medical University General Hospital, Tianjin, 
      China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191128
PL  - England
TA  - J Investig Med
JT  - Journal of investigative medicine : the official publication of the American 
      Federation for Clinical Research
JID - 9501229
RN  - 0 (Interleukin-6)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - *Cell Differentiation
MH  - Cell Line, Tumor
MH  - Cells, Cultured
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Exosomes/*metabolism/physiology/ultrastructure
MH  - Female
MH  - Humans
MH  - Interleukin-6/*metabolism
MH  - Male
MH  - Mesenchymal Stem Cells/*metabolism
MH  - Microscopy, Electron, Transmission
MH  - Middle Aged
MH  - Multiple Myeloma/*metabolism
MH  - Osteoblasts/*cytology
MH  - Real-Time Polymerase Chain Reaction
PMC - PMC6996099
OTO - NOTNLM
OT  - hematology
COIS- Competing interests: None declared.
EDAT- 2019/12/01 06:00
MHDA- 2021/05/01 06:00
PMCR- 2020/02/03
CRDT- 2019/12/01 06:00
PHST- 2019/05/27 00:00 [accepted]
PHST- 2019/12/01 06:00 [pubmed]
PHST- 2021/05/01 06:00 [medline]
PHST- 2019/12/01 06:00 [entrez]
PHST- 2020/02/03 00:00 [pmc-release]
AID - jim-2019-001010 [pii]
AID - 10.1136/jim-2019-001010 [doi]
PST - ppublish
SO  - J Investig Med. 2020 Jan;68(1):45-51. doi: 10.1136/jim-2019-001010. Epub 2019 Nov 
      28.