PMID- 31785241 OWN - NLM STAT- MEDLINE DCOM- 20200113 LR - 20200113 IS - 1879-0631 (Electronic) IS - 0024-3205 (Linking) VI - 240 DP - 2020 Jan 1 TI - Tlr4 participates in the responses of markers of apoptosis, inflammation, and ER stress to different acute exercise intensities in mice hearts. PG - 117107 LID - S0024-3205(19)31034-3 [pii] LID - 10.1016/j.lfs.2019.117107 [doi] AB - BACKGROUND: Toll-like receptor 4 (Tlr4) is recognized due to its role in the immune response. Also, this protein can participate in the signaling pathway of events triggered by physical exercise such as apoptosis, inflammation, and endoplasmic reticulum (ER) stress. The main objective of this study was to evaluate the role of Tlr4 in the markers of these events in the myocardium of mice submitted to acute physical exercise (APE) protocols at different intensities. METHODS: Echocardiogram, RT-qPCR, and immunoblotting technique were used to evaluate the left ventricle of wild-type (WT) and Tlr4 knockout (Tlr4 KO) submitted to APE protocols at 45, 60, and 75% of their maximal velocity. Also, we performed the bioinformatics analysis to establish the connection of heart mRNA levels of Tlr4 with heart genes of inflammation and ER stress of several isogenic strains of BXD mice. RESULTS: Under basal conditions, the Tlr4 deletion diminished the performance, and expression of inflammation and ER stress genes in the left ventricle, but increased the serum levels of CK, Il-17, and Tnf-alpha. Under the same exercise conditions, the Tlr4 deletion reduced the glycemia, serum levels of CK, Il-17, and Tnf-alpha, as well as genes and/or proteins related to apoptosis, inflammation and ER stress in the left ventricle, but increased the levels of CK-mb and LDH, as well as other genes related to apoptosis, inflammation, and ER stress in the left ventricle. CONCLUSION: Altogether, the current findings highlighted the effects of different acute exercise intensities were attenuated in the heart of Tlr4 KO mice. CI - Copyright (c) 2019 Elsevier Inc. All rights reserved. FAU - de Vicente, Larissa G AU - de Vicente LG AD - Postgraduate Program in Rehabilitation and Functional Performance, Ribeirao Preto Medical School, University of Sao Paulo (USP), Ribeirao Preto, Sao Paulo 14040-900, Brazil. FAU - Pinto, Ana P AU - Pinto AP AD - Postgraduate Program in Rehabilitation and Functional Performance, Ribeirao Preto Medical School, University of Sao Paulo (USP), Ribeirao Preto, Sao Paulo 14040-900, Brazil. FAU - Munoz, Vitor R AU - Munoz VR AD - Laboratory of Molecular Biology of Exercise (LaBMEx), School of Applied Sciences, University of Campinas (UNICAMP), Limeira, Sao Paulo 13484-350, Brazil. FAU - Rovina, Rafael L AU - Rovina RL AD - School of Physical Education and Sport of Ribeirao Preto, University of Sao Paulo (USP), Ribeirao Preto, Sao Paulo 14040-900, Brazil. FAU - da Rocha, Alisson L AU - da Rocha AL AD - Postgraduate Program in Rehabilitation and Functional Performance, Ribeirao Preto Medical School, University of Sao Paulo (USP), Ribeirao Preto, Sao Paulo 14040-900, Brazil. FAU - Gaspar, Rafael C AU - Gaspar RC AD - Laboratory of Molecular Biology of Exercise (LaBMEx), School of Applied Sciences, University of Campinas (UNICAMP), Limeira, Sao Paulo 13484-350, Brazil. FAU - da Silva, Lilian E C M AU - da Silva LECM AD - Department of Ophthalmology, Otorhinolaryngology, and Head and Neck Surgery, School of Medicine of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, Sao Paulo 14040-900, Brazil. FAU - Simabuco, Fernando M AU - Simabuco FM AD - Laboratory of Molecular Biology of Exercise (LaBMEx), School of Applied Sciences, University of Campinas (UNICAMP), Limeira, Sao Paulo 13484-350, Brazil. FAU - Frantz, Fabiani G AU - Frantz FG AD - Faculty of Pharmaceutical Sciences of Ribeirao Preto, Department of Clinical, Toxicological, and Bromatological Analysis, University of Sao Paulo (USP), Ribeirao Preto, Sao Paulo 14040-900, Brazil. FAU - Pauli, Jose R AU - Pauli JR AD - Laboratory of Molecular Biology of Exercise (LaBMEx), School of Applied Sciences, University of Campinas (UNICAMP), Limeira, Sao Paulo 13484-350, Brazil. FAU - de Moura, Leandro P AU - de Moura LP AD - Laboratory of Molecular Biology of Exercise (LaBMEx), School of Applied Sciences, University of Campinas (UNICAMP), Limeira, Sao Paulo 13484-350, Brazil. FAU - Cintra, Dennys E AU - Cintra DE AD - Laboratory of Molecular Biology of Exercise (LaBMEx), School of Applied Sciences, University of Campinas (UNICAMP), Limeira, Sao Paulo 13484-350, Brazil. FAU - Ropelle, Eduardo R AU - Ropelle ER AD - Laboratory of Molecular Biology of Exercise (LaBMEx), School of Applied Sciences, University of Campinas (UNICAMP), Limeira, Sao Paulo 13484-350, Brazil. FAU - da Silva, Adelino S R AU - da Silva ASR AD - Postgraduate Program in Rehabilitation and Functional Performance, Ribeirao Preto Medical School, University of Sao Paulo (USP), Ribeirao Preto, Sao Paulo 14040-900, Brazil; School of Physical Education and Sport of Ribeirao Preto, University of Sao Paulo (USP), Ribeirao Preto, Sao Paulo 14040-900, Brazil. Electronic address: adelinosanchez@usp.br. LA - eng PT - Journal Article DEP - 20191127 PL - Netherlands TA - Life Sci JT - Life sciences JID - 0375521 RN - 0 (Il17a protein, mouse) RN - 0 (Interleukin-17) RN - 0 (Tlr4 protein, mouse) RN - 0 (Toll-Like Receptor 4) RN - 0 (Tumor Necrosis Factor-alpha) RN - EC 2.7.3.2 (Creatine Kinase) SB - IM MH - Animals MH - Apoptosis/genetics/*physiology MH - Computational Biology MH - Creatine Kinase/blood MH - Echocardiography MH - Endoplasmic Reticulum Stress/genetics/*physiology MH - Heart/diagnostic imaging/*physiology MH - *Inflammation MH - Interleukin-17/blood MH - Mice MH - Mice, Inbred C57BL MH - Mice, Knockout MH - Physical Exertion/genetics/*physiology MH - Signal Transduction/physiology MH - Toll-Like Receptor 4/genetics/*physiology MH - Tumor Necrosis Factor-alpha/blood OTO - NOTNLM OT - Bioinformatics OT - Echocardiogram OT - Gene expression OT - Protein content and phosphorylation EDAT- 2019/12/01 06:00 MHDA- 2020/01/14 06:00 CRDT- 2019/12/01 06:00 PHST- 2019/10/31 00:00 [received] PHST- 2019/11/25 00:00 [revised] PHST- 2019/11/25 00:00 [accepted] PHST- 2019/12/01 06:00 [pubmed] PHST- 2020/01/14 06:00 [medline] PHST- 2019/12/01 06:00 [entrez] AID - S0024-3205(19)31034-3 [pii] AID - 10.1016/j.lfs.2019.117107 [doi] PST - ppublish SO - Life Sci. 2020 Jan 1;240:117107. doi: 10.1016/j.lfs.2019.117107. Epub 2019 Nov 27.