PMID- 31785643
OWN - NLM
STAT- MEDLINE
DCOM- 20200427
LR  - 20200427
IS  - 1873-2232 (Electronic)
IS  - 0378-4320 (Linking)
VI  - 211
DP  - 2019 Dec
TI  - Sperm cellular and nuclear dynamics associated with bull fertility.
PG  - 106203
LID - S0378-4320(19)30493-2 [pii]
LID - 10.1016/j.anireprosci.2019.106203 [doi]
AB  - The objective of this study was to ascertain cellular characteristics and the 
      dynamics of the sperm chromatin proteins protamine 1 (PRM1) and protamine 2 
      (PRM2) in the sperm of Holstein bulls having a different fertility status. 
      Important sperm variables were analyzed using computer-assisted sperm analysis 
      (CASA). Sperm membrane, acrosome status, DNA integrity were also assessed using 
      propidium iodide (PI), fluorescein isothiocyanate conjugated to Arachis hypogaea 
      (FITC-PNA), and acridine orange (AO) followed by flow cytometry. In addition, 
      abundances of PRM1 and PRM2 were analyzed using flow cytometry experiments. 
      Differences in sperm decondensation capacity were assessed in bulls of varying 
      fertility using a decondensation assay. As determined using CASA, average pathway 
      velocity, amplitude of lateral head displacement and straightness were different 
      (P < 0.05) for sperm from high and low fertility bulls. There, however, were no 
      differences between the high and low fertility bulls for characteristics of sperm 
      plasma membrane, acrosome, and DNA integrity (P >  0.05). Relative abundances of 
      PRM1 and PRM2 in sperm from the high and low fertility bulls were inversely 
      related (P <  0.0001). Percentages of decondensed sperm were different between 
      high and low fertility bulls (P < 0.0001) and total numbers of decondensed sperm 
      were greater in low fertility bulls than high fertility bulls (R(2) = 0.72). 
      Results of the present study are significant because molecular and morphological 
      phenotypes of sperm that were detected affect fertility in livestock species.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Kutchy, Naseer A
AU  - Kutchy NA
AD  - Department of Animal and Dairy Sciences, Mississippi State University, 
      Mississippi State, MS, USA.
FAU - Menezes, Erika S B
AU  - Menezes ESB
AD  - Department of Animal and Dairy Sciences, Mississippi State University, 
      Mississippi State, MS, USA.
FAU - Ugur, Muhammet R
AU  - Ugur MR
AD  - Department of Animal and Dairy Sciences, Mississippi State University, 
      Mississippi State, MS, USA.
FAU - Ul Husna, Asma
AU  - Ul Husna A
AD  - Department of Animal and Dairy Sciences, Mississippi State University, 
      Mississippi State, MS, USA; Department of Zoology, Pir Mehr Ali Shah Arid 
      Agriculture University, Rawalpindi, Pakistan.
FAU - ElDebaky, Hazem
AU  - ElDebaky H
AD  - Department of Animal and Dairy Sciences, Mississippi State University, 
      Mississippi State, MS, USA; National Research Center, Cairo, Egypt.
FAU - Evans, Holly C
AU  - Evans HC
AD  - Department of Animal and Dairy Sciences, Mississippi State University, 
      Mississippi State, MS, USA.
FAU - Beaty, Emily
AU  - Beaty E
AD  - Department of Animal and Dairy Sciences, Mississippi State University, 
      Mississippi State, MS, USA.
FAU - Santos, Fagner C
AU  - Santos FC
AD  - Department of Animal and Dairy Sciences, Mississippi State University, 
      Mississippi State, MS, USA; Department of Animal Science, Federal University of 
      Ceara, Fortaleza, Brazil.
FAU - Tan, Wei
AU  - Tan W
AD  - Flow Cytometry Core Facility, College of Veterinary Medicine, Mississippi State 
      University, Mississippi State, MS, USA.
FAU - Wills, Robert W
AU  - Wills RW
AD  - Department of Pathobiology and Population Medicine, Mississippi State University, 
      Mississippi State, MS, USA.
FAU - Topper, Einko
AU  - Topper E
AD  - Alta Genetics, Inc., Watertown, WI, USA.
FAU - Kaya, Abdullah
AU  - Kaya A
AD  - Department of Reproduction and Artificial Insemination, Faculty of Veterinary 
      Medicine, Selcuk University, Konya, Turkey.
FAU - Moura, Arlindo A
AU  - Moura AA
AD  - Department of Animal Science, Federal University of Ceara, Fortaleza, Brazil.
FAU - Memili, Erdogan
AU  - Memili E
AD  - Department of Animal and Dairy Sciences, Mississippi State University, 
      Mississippi State, MS, USA. Electronic address: em149@ads.msstate.edu.
LA  - eng
PT  - Journal Article
DEP - 20191018
PL  - Netherlands
TA  - Anim Reprod Sci
JT  - Animal reproduction science
JID - 7807205
RN  - 0 (Chromatin)
RN  - 0 (Protamines)
SB  - IM
MH  - Animals
MH  - Cattle/*physiology
MH  - Cell Nucleus/physiology
MH  - Chromatin/physiology
MH  - Fertility/*physiology
MH  - Gene Expression Regulation
MH  - Male
MH  - Protamines/genetics/metabolism
MH  - Spermatozoa/cytology/*physiology
OTO - NOTNLM
OT  - Chromatin decondensation
OT  - Male fertility
OT  - Nuclear proteins
OT  - Protamines
OT  - Sperm DNA integrity
EDAT- 2019/12/02 06:00
MHDA- 2020/04/28 06:00
CRDT- 2019/12/02 06:00
PHST- 2019/05/26 00:00 [received]
PHST- 2019/09/18 00:00 [revised]
PHST- 2019/10/09 00:00 [accepted]
PHST- 2019/12/02 06:00 [entrez]
PHST- 2019/12/02 06:00 [pubmed]
PHST- 2020/04/28 06:00 [medline]
AID - S0378-4320(19)30493-2 [pii]
AID - 10.1016/j.anireprosci.2019.106203 [doi]
PST - ppublish
SO  - Anim Reprod Sci. 2019 Dec;211:106203. doi: 10.1016/j.anireprosci.2019.106203. 
      Epub 2019 Oct 18.