PMID- 31786843
OWN - NLM
STAT- MEDLINE
DCOM- 20200427
LR  - 20200427
IS  - 2241-6293 (Electronic)
IS  - 1107-0625 (Linking)
VI  - 24
IP  - 5
DP  - 2019 Sep-Oct
TI  - Evodiamine exerts anticancer effects via induction of apoptosis and autophagy and 
      suppresses the migration and invasion of human colon cancer cells.
PG  - 1824-1829
AB  - PURPOSE: Colon cancer ranks as the fourth common type of cancer and is 
      responsible for significant morbidity and mortality throughout the world. Late 
      diagnosis and the rarity of potent and safer chemotherapeutic drugs and efficient 
      therapeutic targets create severe obstacle in the treatment of colon cancer. This 
      study was undertaken to examine the anticancer effects of Evodiamine against 
      human colon cancer cells. METHODS: The proliferation rate of the SW480 colon 
      cancer cells was monitored by MTT assay. Apoptosis was detected by Annexin 
      V/propidium iodide (PI) and acridine orange (AO)/ethidium bromide (EB) staining. 
      Transmission electron microscopy (TEM) was used for detection of autophagy. Cell 
      migration and invasion was detected by wound healing and transwell assays, 
      respectively. Protein expression was determined by western blotting. RESULTS: 
      Evodiamine suppressed the proliferation of the SW480 colon cancer cells and 
      exhibited an IC50 of 10 microM. The cytotoxic effects of Evodiamine were found to be 
      comparatively lower against the normal CDD-18Co colon cells as evidenced from the 
      IC50 of 100 microM. AO/EB staining showed that Evodiamine caused apoptosis of the 
      SW480 cells and the percentage of the apoptotic SW480 cells increased with 
      increase in the Evodiamine concentration as indicated by annexin V/PI staining. 
      Evodiamine-induced apoptosis was also accompanied by upregulation of caspase-3 
      and Bax and suppression of Bcl-2. TEM analysis showed that Evodiamine also 
      activated autophagy in the SW480 cells by enhancing the expression of LC3 II and 
      Beclin 1. The wound assay showed that Evodiamine suppressed the migration of the 
      SW480 cells. Evodiamine also reduced the invasion potential of the SW480 cells as 
      suggested by the transwell assay. CONCLUSION: The findings of the present study 
      suggest that Evodiamine is a potent anticancer agent and may prove beneficial in 
      the development of systemic therapy of colon cancer.
FAU - Wang, Dan
AU  - Wang D
AD  - Department of General Surgery, the third People's Hospital of Dalian, Dalian 
      Medical University, Dalian, Liaoning Province, China, 116034.
FAU - Ge, Shuke
AU  - Ge S
FAU - Chen, Zhe
AU  - Chen Z
FAU - Song, Yongwei
AU  - Song Y
LA  - eng
PT  - Journal Article
PL  - Cyprus
TA  - J BUON
JT  - Journal of B.U.ON. : official journal of the Balkan Union of Oncology
JID - 100883428
RN  - 0 (Antineoplastic Agents, Phytogenic)
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (Autophagy-Related Proteins)
RN  - 0 (Quinazolines)
RN  - C01825BVNL (evodiamine)
SB  - IM
MH  - Antineoplastic Agents, Phytogenic/*pharmacology
MH  - Apoptosis/*drug effects
MH  - Apoptosis Regulatory Proteins/metabolism
MH  - Autophagy/*drug effects
MH  - Autophagy-Related Proteins/metabolism
MH  - Cell Line, Tumor
MH  - Cell Movement/*drug effects
MH  - Cell Proliferation/drug effects
MH  - Colorectal Neoplasms/*drug therapy/metabolism/ultrastructure
MH  - Humans
MH  - Neoplasm Invasiveness
MH  - Quinazolines/*pharmacology
MH  - Signal Transduction
EDAT- 2019/12/02 06:00
MHDA- 2020/04/28 06:00
CRDT- 2019/12/02 06:00
PHST- 2019/12/02 06:00 [entrez]
PHST- 2019/12/02 06:00 [pubmed]
PHST- 2020/04/28 06:00 [medline]
PST - ppublish
SO  - J BUON. 2019 Sep-Oct;24(5):1824-1829.