PMID- 31786883
OWN - NLM
STAT- MEDLINE
DCOM- 20200428
LR  - 20200428
IS  - 2241-6293 (Electronic)
IS  - 1107-0625 (Linking)
VI  - 24
IP  - 5
DP  - 2019 Sep-Oct
TI  - MicroRNA-15 regulates the proliferation, migration and invasion of thyroid cancer 
      cells by targeting Bcl-2.
PG  - 2114-2119
AB  - PURPOSE: Thyroid cancer causes significant mortality and 1-1.5% of all the new 
      diagnosed cancers are thyroid cancers. The incidence of thyroid cancer is 
      increasing at an alarming rate and therapeutic targets are lacking. This study 
      was undertaken to investigate the role and therapeutic implications of microRNA 
      (miR)-15 in thyroid cancer. METHODS: Expression analysis was performed by 
      qRT-PCR. Transfections were performed by Lipofectamine 2000 reagent. The cell 
      viability was determined by MTT assay. Apoptosis was detected by acridine orange 
      (AO)/ethidium bromide (EB). The percentage of apoptotic cells was estimated by 
      annexin V/ propidium iodide (PI) staining. Wound healing and transwell assays 
      were used to monitor the cell migration and invasion. Protein expression was 
      determined by western blotting. RESULTS: The expression of miR-15 was found 
      significantly decreased in thyroid cancer cells. Ectopic expression of miR-15 
      promoted the apoptosis of MDA-T35 thyroid cancer cells. The percentage of 
      apoptotic MDA-T35 cells was 1.9% in thyroid cancer and 40.1% in miR-15 mimics 
      transfected cells. The apoptosis promoted by miR-15 overexpression was also 
      associated with enhancement of Bax and depletion of Bcl-2 in MDA-T35 cells. The 
      TargetScan analysis showed Bcl-2 to be the target of miR-15. Additionally, the 
      expression of Bcl-2 was also enhanced in all the thyroid cancer cells and miR-15 
      ectopic expression could cause suppression of the Bcl-2 expression in MDA-T35 
      cells. The wound healing assay showed that miR-5 overexpression caused decrease 
      in the migration of MDA-T35 cells while the transwell assays showed decline in 
      the invasion of miR-15 mimics transfected MDA-T35 cells. CONCLUSION: To sum up, 
      miR-15 may exhibit therapeutic implications in thyroid cancer and may prove 
      useful in thyroid cancer treatment.
FAU - Lu, Zhongwu
AU  - Lu Z
AD  - Department of Head and Neck Surgery, Fudan University, Shanghai Cancer Center, 
      Shanghai 200032, China.
FAU - Wu, Zhiqiang
AU  - Wu Z
FAU - Hu, Jiaqia
AU  - Hu J
FAU - Wei, Wenjun
AU  - Wei W
FAU - Ma, Ben
AU  - Ma B
FAU - Wen, Duo
AU  - Wen D
LA  - eng
PT  - Journal Article
PL  - Cyprus
TA  - J BUON
JT  - Journal of B.U.ON. : official journal of the Balkan Union of Oncology
JID - 100883428
RN  - 0 (BCL2 protein, human)
RN  - 0 (MIRN15 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
SB  - IM
MH  - Apoptosis/genetics
MH  - Cell Line, Tumor
MH  - Cell Movement/genetics
MH  - Cell Proliferation/*genetics
MH  - Cell Survival/genetics
MH  - Gene Expression Regulation, Neoplastic/genetics
MH  - Humans
MH  - MicroRNAs/*genetics
MH  - Neoplasm Invasiveness/genetics/pathology
MH  - Proto-Oncogene Proteins c-bcl-2/*genetics
MH  - Thyroid Neoplasms/*genetics/pathology
EDAT- 2019/12/02 06:00
MHDA- 2020/04/29 06:00
CRDT- 2019/12/02 06:00
PHST- 2019/12/02 06:00 [entrez]
PHST- 2019/12/02 06:00 [pubmed]
PHST- 2020/04/29 06:00 [medline]
PST - ppublish
SO  - J BUON. 2019 Sep-Oct;24(5):2114-2119.