PMID- 31787236
OWN - NLM
STAT- MEDLINE
DCOM- 20200831
LR  - 20200831
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 522
IP  - 3
DP  - 2020 Feb 12
TI  - Protective role of c-Jun NH(2)-terminal kinase-associated leucine zipper protein 
      (JLP) in curcumin-induced cancer cell death.
PG  - 697-703
LID - S0006-291X(19)32282-X [pii]
LID - 10.1016/j.bbrc.2019.11.154 [doi]
AB  - Previous studies have established the antitumor activity of curcumin, a major 
      component of turmeric. Increasing evidence indicates that curcumin induces 
      autophagy, the activation of mitogen-activated protein kinase (MAPK) 
      intracellular signaling pathways, and reactive oxygen species (ROS)-mediated cell 
      death. The c-Jun NH(2)-terminal kinase (JNK)-associated leucine zipper protein 
      (JLP), a scaffold protein for MAPK signaling pathways, has been identified as a 
      candidate biomarker for cancer. In this study, we explored the role of JLP in 
      curcumin-induced cancer cell death. We found that JLP knockdown (KD) increases 
      cell death and intracellular ROS levels. Furthermore, JLP KD impaired lysosomal 
      accumulation around perinuclear regions, which led to the inhibition of 
      autophagosome-lysosome fusion, and attenuated p38 MAPK activation in 
      curcumin-treated cells. The decreases in cell viability and p38 MAPK activation 
      were reversed by expressing wild-type JLP but not a JLP mutant lacking the p38 
      MAPK-binding domain. In addition, the inactivation of a key gene involved in 
      autophagy increased sensitivity to curcumin-induced cell death. Together, these 
      results suggest that JLP mediates the induction of autophagy by regulating 
      lysosome positioning and p38 MAPK signaling, indicating an overall protective 
      role in curcumin-induced ROS-mediated cancer cell death.
CI  - Copyright (c) 2019 Elsevier Inc. All rights reserved.
FAU - Boldbaatar, Jambaldorj
AU  - Boldbaatar J
AD  - Division of Molecular Cell Signaling, Cancer Research Institute, Kanazawa 
      University, Kanazawa, Japan.
FAU - Gunarta, I Ketut
AU  - Gunarta IK
AD  - Division of Molecular Cell Signaling, Cancer Research Institute, Kanazawa 
      University, Kanazawa, Japan.
FAU - Suzuki, Ryusuke
AU  - Suzuki R
AD  - Division of Molecular Cell Signaling, Cancer Research Institute, Kanazawa 
      University, Kanazawa, Japan.
FAU - Erdenebaatar, Purev
AU  - Erdenebaatar P
AD  - Division of Molecular Cell Signaling, Cancer Research Institute, Kanazawa 
      University, Kanazawa, Japan.
FAU - Davaakhuu, Gantulga
AU  - Davaakhuu G
AD  - Laboratory of Molecular Biology, Institute of General and Experimental Biology, 
      Mongolian Academy of Sciences, Ulaanbaatar, Mongolia.
FAU - Hohjoh, Hirohiko
AU  - Hohjoh H
AD  - Department of Molecular Pharmacology, National Institute of Neuroscience, NCNP, 
      Kodaira, Tokyo, Japan.
FAU - Yoshioka, Katsuji
AU  - Yoshioka K
AD  - Division of Molecular Cell Signaling, Cancer Research Institute, Kanazawa 
      University, Kanazawa, Japan. Electronic address: katsuji@staff.kanazawa-u.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191128
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (SPAG9 protein, human)
RN  - IT942ZTH98 (Curcumin)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing/*metabolism
MH  - Antineoplastic Agents/*pharmacology
MH  - Autophagy/drug effects
MH  - Cell Death/drug effects
MH  - Cell Line, Tumor
MH  - Curcumin/*pharmacology
MH  - Humans
MH  - Neoplasms/*drug therapy/metabolism
MH  - Reactive Oxygen Species/metabolism
OTO - NOTNLM
OT  - Autophagy
OT  - Cell death
OT  - Lysosome
OT  - MAP kinase
OT  - ROS
EDAT- 2019/12/04 06:00
MHDA- 2020/09/01 06:00
CRDT- 2019/12/03 06:00
PHST- 2019/11/13 00:00 [received]
PHST- 2019/11/22 00:00 [accepted]
PHST- 2019/12/04 06:00 [pubmed]
PHST- 2020/09/01 06:00 [medline]
PHST- 2019/12/03 06:00 [entrez]
AID - S0006-291X(19)32282-X [pii]
AID - 10.1016/j.bbrc.2019.11.154 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2020 Feb 12;522(3):697-703. doi: 
      10.1016/j.bbrc.2019.11.154. Epub 2019 Nov 28.