PMID- 31787253
OWN - NLM
STAT- MEDLINE
DCOM- 20200924
LR  - 20200924
IS  - 1618-0372 (Electronic)
IS  - 0065-1281 (Linking)
VI  - 122
IP  - 1
DP  - 2020 Jan
TI  - Involvement of proliferative and apoptotic factors in the development of hindgut 
      in rat fetuses with ethylenethiourea-induced anorectal malformations.
PG  - 151466
LID - S0065-1281(19)30452-0 [pii]
LID - 10.1016/j.acthis.2019.151466 [doi]
AB  - BACKGROUND: Anorectal malformations (ARMs) are common congenital malformations of 
      the terminal digestive tract, but little is known regarding their pathogenesis. 
      Aberrant cell proliferation/apoptosis are believed to be involved in ARMs. 
      However, there are no studies on proliferation/apoptosis-related genes. PURPOSE: 
      We aimed to investigate the spatiotemporal expression patterns of two 
      proliferation/apoptosis-related genes (MYC proto-oncogene and tumor protein p53) 
      and explore their potential functions in the hindguts of ethylene 
      thiourea-induced ARMs rat fetuses. METHODS: MYC and p53 expression was evaluated 
      using immunohistochemical staining, western blotting, and quantitative real-time 
      polymerase chain reaction (RT-qPCR). Terminal deoxynucleotidyl transferase 
      mediated dUTP nick end labeling (TUNEL) and p53 costaining were performed to 
      assay the colocalization of apoptotic and p53-expressing cells. RESULTS: Rat 
      fetuses with ARMs displayed fusion failure of the urogenital septum and cloacal 
      membrane. In the control group, MYC was persistently expressed from gestational 
      day (GD)14 to GD16 and distributed throughout the hindgut, while p53 was weakly 
      detected in the terminal segment of the urethra and hindgut; in the ARMs group, 
      MYC expression was obviously reduced, while p53 was widely and highly expressed 
      in the urethra and hindgut. Western blotting and RT-qPCR confirmed the decrease 
      in MYC and increase in p53 expression in ARMs. TUNEL and p53 co-staining revealed 
      considerable overlap between apoptotic and p53-expressing cells. CONCLUSION: The 
      expression patterns of c-myc and p53 were disrupted in ARMs rat embryos, and the 
      downregulation of c-myc and upregulation of p53 might be related to the 
      development of ARMs at the key time points of ARMs morphogenesis.
CI  - Copyright (c) 2019 The Authors. Published by Elsevier GmbH.. All rights reserved.
FAU - Long, Caiyun
AU  - Long C
AD  - Department of Pediatric Surgery, Shengjing Hospital of China Medical University, 
      Shenyang, Liaoning 110004, PR China.
FAU - Xiao, Yunxia
AU  - Xiao Y
AD  - Department of Pediatric Surgery, Shengjing Hospital of China Medical University, 
      Shenyang, Liaoning 110004, PR China.
FAU - Li, Siying
AU  - Li S
AD  - Department of Pediatric Surgery, Shengjing Hospital of China Medical University, 
      Shenyang, Liaoning 110004, PR China.
FAU - Tang, Xiaobing
AU  - Tang X
AD  - Department of Pediatric Surgery, Shengjing Hospital of China Medical University, 
      Shenyang, Liaoning 110004, PR China.
FAU - Yuan, Zhengwei
AU  - Yuan Z
AD  - The Key Laboratory of Health Ministry for Congenital Malformation, Shengjing 
      Hospital of China Medical University, Shenyang, Liaoning 110004, PR China.
FAU - Bai, Yuzuo
AU  - Bai Y
AD  - Department of Pediatric Surgery, Shengjing Hospital of China Medical University, 
      Shenyang, Liaoning 110004, PR China. Electronic address: baiyz1216@126.com.
LA  - eng
PT  - Journal Article
DEP - 20191128
PL  - Germany
TA  - Acta Histochem
JT  - Acta histochemica
JID - 0370320
RN  - 0 (Proto-Oncogene Proteins c-myc)
RN  - 0 (Tp53 protein, rat)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - 24FOJ4N18S (Ethylenethiourea)
SB  - IM
MH  - Animals
MH  - *Anorectal Malformations/chemically induced/embryology/pathology
MH  - Apoptosis/drug effects
MH  - Cell Proliferation/drug effects
MH  - Ethylenethiourea/*toxicity
MH  - Female
MH  - *Fetus/embryology/pathology
MH  - Gene Expression Regulation, Developmental/*drug effects
MH  - *Intestines/embryology/pathology
MH  - Pregnancy
MH  - Proto-Oncogene Proteins c-myc/*biosynthesis
MH  - Rats
MH  - Rats, Wistar
MH  - Tumor Suppressor Protein p53/*biosynthesis
OTO - NOTNLM
OT  - Anorectal malformations
OT  - Development
OT  - Hindgut
OT  - Rat
OT  - c-myc
OT  - p53
EDAT- 2019/12/04 06:00
MHDA- 2020/09/25 06:00
CRDT- 2019/12/03 06:00
PHST- 2019/07/13 00:00 [received]
PHST- 2019/10/16 00:00 [revised]
PHST- 2019/11/14 00:00 [accepted]
PHST- 2019/12/04 06:00 [pubmed]
PHST- 2020/09/25 06:00 [medline]
PHST- 2019/12/03 06:00 [entrez]
AID - S0065-1281(19)30452-0 [pii]
AID - 10.1016/j.acthis.2019.151466 [doi]
PST - ppublish
SO  - Acta Histochem. 2020 Jan;122(1):151466. doi: 10.1016/j.acthis.2019.151466. Epub 
      2019 Nov 28.