PMID- 31789387
OWN - NLM
STAT- MEDLINE
DCOM- 20201023
LR  - 20240322
IS  - 1791-2423 (Electronic)
IS  - 1019-6439 (Print)
IS  - 1019-6439 (Linking)
VI  - 56
IP  - 1
DP  - 2020 Jan
TI  - Interaction of YAP1 and mTOR promotes bladder cancer progression.
PG  - 232-242
LID - 10.3892/ijo.2019.4922 [doi]
AB  - Yes‑associated protein 1 (YAP1) and mammalian target of rapamycin (mTOR) 
      signaling pathways have been found to be deregulated in bladder cancer and 
      accelerate the malignant progression of bladder cancer. However, the crosstalk 
      between YAP1 and mTOR and its role in bladder cancer progression remains unclear. 
      The aim of the present study was to investigate this crosstalk and the results 
      revealed that the expression of YAP1 and mTOR was elevated in bladder cancer 
      tissues compared with that in adjacent normal tissues. Knockdown of either mTOR 
      or YAP1 with siRNA transfection significantly repressed the proliferation ability 
      and induced apoptosis of HT‑1376 and J82 bladder cancer cells, particularly when 
      YAP1 and mTOR were downregulated simultaneously. Upregulation of mTOR increased 
      the mRNA and protein levels of YAP1 and enhanced its nuclear accumulation. In 
      turn, YAP1 upregulation increased mTOR expression, reduced its protein 
      degradation and increased its stability. In addition, immunofluorescence and 
      Duolink assays demonstrated that YAP1 and mTOR were co‑localized in the nucleus. 
      Immunoprecipitation assay demonstrated that the YAP1 protein was able to bind to 
      the mTOR protein. Moreover, YAP1 combined with S‑phase kinase‑associated 
      protein 2 (SKP2) and positively regulated its expression. Furthermore, the 
      promotion of cell growth and inhibition of cell apoptosis induced by YAP1 
      overexpression were abolished when SKP2 was downregulated in HT‑1376 and 
      J82 cells. Taken together, the findings of the present study indicated that the 
      crosstalk between YAP1 and mTOR plays a pivotal role in accelerating the 
      progression of bladder cancer, which may provide new insights into the role of 
      the YAP1/mTOR axis in the occurrence and development of bladder cancer.
FAU - Xu, Mingxi
AU  - Xu M
AD  - Department of Urology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong 
      University School of Medicine, Shanghai 200011, P.R. China.
FAU - Gu, Meng
AU  - Gu M
AD  - Department of Urology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong 
      University School of Medicine, Shanghai 200011, P.R. China.
FAU - Zhou, Juan
AU  - Zhou J
AD  - Department of Urology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong 
      University School of Medicine, Shanghai 200011, P.R. China.
FAU - Da, Jun
AU  - Da J
AD  - Department of Urology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong 
      University School of Medicine, Shanghai 200011, P.R. China.
FAU - Wang, Zhong
AU  - Wang Z
AD  - Department of Urology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong 
      University School of Medicine, Shanghai 200011, P.R. China.
LA  - eng
PT  - Journal Article
PT  - Retracted Publication
DEP - 20191125
PL  - Greece
TA  - Int J Oncol
JT  - International journal of oncology
JID - 9306042
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Transcription Factors)
RN  - 0 (YAP-Signaling Proteins)
RN  - 0 (YAP1 protein, human)
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
RIN - Int J Oncol. 2024 May;64(5):. PMID: 38516763
MH  - Adaptor Proteins, Signal Transducing/genetics/*metabolism
MH  - Apoptosis
MH  - Biomarkers, Tumor/genetics/*metabolism
MH  - Cell Proliferation
MH  - Disease Progression
MH  - *Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Prognosis
MH  - Protein Interaction Domains and Motifs
MH  - TOR Serine-Threonine Kinases/genetics/*metabolism
MH  - Transcription Factors/genetics/*metabolism
MH  - Tumor Cells, Cultured
MH  - Urinary Bladder Neoplasms/genetics/metabolism/*pathology
MH  - YAP-Signaling Proteins
PMC - PMC6910214
EDAT- 2019/12/04 06:00
MHDA- 2020/10/24 06:00
PMCR- 2019/11/25
CRDT- 2019/12/03 06:00
PHST- 2018/11/16 00:00 [received]
PHST- 2019/11/07 00:00 [accepted]
PHST- 2019/12/04 06:00 [pubmed]
PHST- 2020/10/24 06:00 [medline]
PHST- 2019/12/03 06:00 [entrez]
PHST- 2019/11/25 00:00 [pmc-release]
AID - ijo-56-01-0232 [pii]
AID - 10.3892/ijo.2019.4922 [doi]
PST - ppublish
SO  - Int J Oncol. 2020 Jan;56(1):232-242. doi: 10.3892/ijo.2019.4922. Epub 2019 Nov 
      25.