PMID- 31792610
OWN - NLM
STAT- MEDLINE
DCOM- 20201118
LR  - 20210913
IS  - 1437-1596 (Electronic)
IS  - 0937-9827 (Linking)
VI  - 134
IP  - 2
DP  - 2020 Mar
TI  - Forensic pathological study on temporal appearance of dendritic cells in skin 
      wounds.
PG  - 597-601
LID - 10.1007/s00414-019-02185-z [doi]
AB  - Dendritic cells (DCs) can essentially contribute to innate and adaptive immune 
      system in various organs. A double-color immunofluorescence analysis was carried 
      out with anti-CD11c and -HLA-DRalpha antibodies to detect DCs in 53 skin wounds 
      (their postinfliction intervals: group I, 0-3 days; group II, 4-7 days; group 
      III, 9-14 days; and group IV, 17-21 days). CD11c(+)HLA-DRalpha(+) DCs were first 
      observed in skin wounds with postinfliction intervals of 3 days, and the DC 
      numbers were found to be elevated in skin wounds with the subsequent increase in 
      postinfliction intervals. Semi-quantitative morphometric analyses showed that the 
      DC number was the highest in the 12-day-old wound. More than 50 DCs were present 
      in 8 of 10 samples (80%) in group II and 14 of 16 samples (87.5%) in group III, 
      and there was no difference between the two groups. Thus, the presence of DCs in 
      a skin wound was possibly estimated as postinfliction intervals of at least 3 
      days. Furthermore, when a skin wound contained > 50 DCs, its age would be judged 
      as 4-14 days. Collectively, the appearance of DCs in human skin wounds may 
      provide useful information in determining the age of a wound.
FAU - Kuninaka, Yumi
AU  - Kuninaka Y
AD  - Department of Forensic Medicine, Wakayama Medical University, 811-1 Kimiidera, 
      Wakayama, 641-8509, Japan.
FAU - Ishida, Yuko
AU  - Ishida Y
AD  - Department of Forensic Medicine, Wakayama Medical University, 811-1 Kimiidera, 
      Wakayama, 641-8509, Japan.
FAU - Nosaka, Mizuho
AU  - Nosaka M
AD  - Department of Forensic Medicine, Wakayama Medical University, 811-1 Kimiidera, 
      Wakayama, 641-8509, Japan.
FAU - Shimada, Emi
AU  - Shimada E
AD  - Department of Forensic Medicine, Wakayama Medical University, 811-1 Kimiidera, 
      Wakayama, 641-8509, Japan.
FAU - Kimura, Akihiko
AU  - Kimura A
AD  - Department of Forensic Medicine, Wakayama Medical University, 811-1 Kimiidera, 
      Wakayama, 641-8509, Japan.
FAU - Ozaki, Mitsunori
AU  - Ozaki M
AD  - Department of Neurological Surgery, Wakayama Medical University, 811-1 Kimiidera, 
      Wakayama, 641-8509, Japan.
FAU - Hata, Satoshi
AU  - Hata S
AD  - Department of Forensic Medicine, Wakayama Medical University, 811-1 Kimiidera, 
      Wakayama, 641-8509, Japan.
FAU - Michiue, Tomomi
AU  - Michiue T
AD  - Department of Forensic Medicine, Wakayama Medical University, 811-1 Kimiidera, 
      Wakayama, 641-8509, Japan.
FAU - Yamamoto, Hiroki
AU  - Yamamoto H
AD  - Department of Forensic Medicine, Wakayama Medical University, 811-1 Kimiidera, 
      Wakayama, 641-8509, Japan.
FAU - Furukawa, Fukumi
AU  - Furukawa F
AD  - Department of Forensic Medicine, Wakayama Medical University, 811-1 Kimiidera, 
      Wakayama, 641-8509, Japan.
FAU - Eisenmenger, Wolfgang
AU  - Eisenmenger W
AD  - Institute of Legal Medicine, University of Munich, Nubetabaumstrabetae 26, 80336, 
      Munich, Germany.
FAU - Kondo, Toshikazu
AU  - Kondo T
AUID- ORCID: 0000-0002-5059-8309
AD  - Department of Forensic Medicine, Wakayama Medical University, 811-1 Kimiidera, 
      Wakayama, 641-8509, Japan. kondot@wakayama-med.ac.jp.
LA  - eng
GR  - Grant-in-Aid for Scientific Research (A)/Ministry of Education, Science, Sports, 
      and Culture of Japan/
GR  - Exploratory Research/Ministry of Education, Science, Sports, and Culture of 
      Japan/
PT  - Journal Article
DEP - 20191202
PL  - Germany
TA  - Int J Legal Med
JT  - International journal of legal medicine
JID - 9101456
RN  - 0 (CD11c Antigen)
RN  - 0 (HLA-DR Antigens)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - CD11c Antigen
MH  - Child
MH  - *Dendritic Cells
MH  - Fluorescent Antibody Technique
MH  - *Forensic Pathology
MH  - HLA-DR Antigens
MH  - Humans
MH  - Middle Aged
MH  - Skin/*injuries
MH  - Soft Tissue Injuries/immunology
MH  - Time Factors
MH  - Wound Healing/*immunology
MH  - Wounds, Penetrating/immunology
MH  - Young Adult
OTO - NOTNLM
OT  - Dendritic cells
OT  - Forensic pathology
OT  - Wound age determination
EDAT- 2019/12/04 06:00
MHDA- 2020/11/20 06:00
CRDT- 2019/12/04 06:00
PHST- 2019/05/29 00:00 [received]
PHST- 2019/10/15 00:00 [accepted]
PHST- 2019/12/04 06:00 [pubmed]
PHST- 2020/11/20 06:00 [medline]
PHST- 2019/12/04 06:00 [entrez]
AID - 10.1007/s00414-019-02185-z [pii]
AID - 10.1007/s00414-019-02185-z [doi]
PST - ppublish
SO  - Int J Legal Med. 2020 Mar;134(2):597-601. doi: 10.1007/s00414-019-02185-z. Epub 
      2019 Dec 2.