PMID- 31794631
OWN - NLM
STAT- MEDLINE
DCOM- 20210208
LR  - 20210208
IS  - 1539-3429 (Electronic)
IS  - 1470-8175 (Linking)
VI  - 48
IP  - 2
DP  - 2020 Mar
TI  - A laboratory practice that uses the polymerase chain reaction-sequence specific 
      priming technique to rapidly screen for HLA-DR2 allotype from germline DNA in 
      immunology course for undergraduate medical students.
PG  - 175-180
LID - 10.1002/bmb.21322 [doi]
AB  - In this article, we describe an in-house polymerase chain reaction-sequence 
      specific priming (PCR-SSP) assay designed for undergraduate medical students as 
      part of the experimental pathogen biology and immunology (EPBI) course. It 
      screens human leukocyte antigen (HLA)-DR2 allotype from genomic DNA samples using 
      a rapid and single-tube PCR technique, yielding definitive typing result without 
      conventional post-amplification step like probing or Sanger sequencing. This 
      laboratory exercise offers the undergraduate medical students an opportunity to 
      learn about current molecular biology techniques in HLA genotyping with limited 
      effort and cost, in addition to a better understanding of concepts presented in 
      the classroom lectures. Upon completing this experiment module, the students show 
      statistically significant improvement in several key indexes, such as the 
      knowledge about the mainstream HLA DNA typing techniques, awareness of the 
      relevance of this knowledge for their future scientific research, 
      immunogenetics-related basic laboratory skills they acquire, and interest and 
      desire for mastering this assay (all p < .05). This easy to implement set of 
      experiments is composed of a two-session lab module occupying eight teaching 
      hours, and has been run successfully in our laboratory.
CI  - (c) 2019 International Union of Biochemistry and Molecular Biology.
FAU - Li, LiXin
AU  - Li L
AD  - Laboratory of Cellular and Molecular Biology, College of Basic Medical Sciences, 
      Central South University, Changsha, Hunan, China.
FAU - Tian, Wei
AU  - Tian W
AUID- ORCID: 0000-0002-8109-9924
AD  - Laboratory of Cellular and Molecular Biology, College of Basic Medical Sciences, 
      Central South University, Changsha, Hunan, China.
AD  - Immunogenetics Research Group, Department of Immunology, College of Basic Medical 
      Sciences, Central South University, Changsha, Hunan, China.
FAU - Zhang, Jie
AU  - Zhang J
AD  - Laboratory of Cellular and Molecular Biology, College of Basic Medical Sciences, 
      Central South University, Changsha, Hunan, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191203
PL  - United States
TA  - Biochem Mol Biol Educ
JT  - Biochemistry and molecular biology education : a bimonthly publication of the 
      International Union of Biochemistry and Molecular Biology
JID - 100970605
RN  - 0 (HLA-DR2 Antigen)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Alleles
MH  - *Curriculum
MH  - DNA/*genetics
MH  - Education, Medical, Undergraduate
MH  - Germ Cells/*metabolism
MH  - HLA-DR2 Antigen/*genetics
MH  - Humans
MH  - *Laboratories
MH  - *Polymerase Chain Reaction
MH  - *Sequence Analysis, DNA
MH  - Students, Medical
OTO - NOTNLM
OT  - PCR-SSP
OT  - human leukocyte antigen
OT  - immunogenetics
OT  - laboratory exercises
EDAT- 2019/12/04 06:00
MHDA- 2021/02/09 06:00
CRDT- 2019/12/04 06:00
PHST- 2019/07/21 00:00 [received]
PHST- 2019/10/20 00:00 [revised]
PHST- 2019/11/15 00:00 [accepted]
PHST- 2019/12/04 06:00 [pubmed]
PHST- 2021/02/09 06:00 [medline]
PHST- 2019/12/04 06:00 [entrez]
AID - 10.1002/bmb.21322 [doi]
PST - ppublish
SO  - Biochem Mol Biol Educ. 2020 Mar;48(2):175-180. doi: 10.1002/bmb.21322. Epub 2019 
      Dec 3.