PMID- 31795547 OWN - NLM STAT- MEDLINE DCOM- 20191224 LR - 20200108 IS - 1673-0860 (Print) IS - 1673-0860 (Linking) VI - 54 IP - 11 DP - 2019 Nov 7 TI - [Expression of amphiregulin in different types of nasal polyps and its correlation with tissue remodeling]. PG - 850-856 LID - 10.3760/cma.j.issn.1673-0860.2019.11.009 [doi] AB - Objective: To explore the expression of amphiregulin (AREG) in nasal polyps patients with different degrees of eosinophil infiltration, and to analyze the correlation between AREG and tissue remodeling. Methods: Forty-eight patients underwent endoscopic sinus surgery in the Department of Otorhinolaryngology Head and Neck Surgery, Remin Hospital, Wuhan University from July 2017 to August 2018 were recruited, including 40 males and 8 females, aged from 16 to 60 years old. The subjects were divided into three groups: control group (n=14), eosinophilic chronic sinusitis with nasal polyps (ECRSwNP) group (n=19) and noneosinophilic chronic rhinosinusitis with nasal polyps (non-ECRSwNP) group (n=15). The relative expression of AREG in nasal mucosa was detected by Western blot assay and immunohistochemical staining. Tissue remodeling was detected by HE staining, AB-PAS staining and Masson staining. Kruskal-Wallis test was used for comparison among multiple groups, and Spearman correlation analysis was conducted between the expression level of AREG and the related indexes of tissue remodeling. Results: The expression of AREG in ECRSwNP group was significantly higher than that in non-ECRSwNP group and control group (median protein expression of Western blot was 1.592 vs 0.617 vs0.582, all P<0.05). The degree of epithelial injury and goblet cell metaplasia in ECRSwNP group was significantly higher than that in control group (all P<0.05), the percentage of collagen fibrosis area in ECRSwNP group was significantly lower than that in control group (P=0.01). In chronic rhinosinusitis with nasal polyps (CRSwNP) patients, the area of mucous glands was negatively correlated with the expression of AREG (r=-0.616, P<0.05), and the percentage of collagen fibrosis area was negatively correlated with the expression of AREG (r=-0.738, P<0.05). Conclusion: The expression of AREG is higher in ECRSwNP patients, which is related to the process of tissue remodeling. FAU - Gao, L AU - Gao L AD - Department of Otorhinolaryngology Head and Neck Surgery, Renmin Hospital, Wuhan University, Wuhan 430060, China. FAU - Xu, Y AU - Xu Y AD - Department of Otorhinolaryngology Head and Neck Surgery, Renmin Hospital, Wuhan University, Wuhan 430060, China. FAU - Zhang, W AU - Zhang W AD - Department of Otorhinolaryngology Head and Neck Surgery, Renmin Hospital, Wuhan University, Wuhan 430060, China. FAU - Xiang, R AU - Xiang R AD - Department of Otorhinolaryngology Head and Neck Surgery, Renmin Hospital, Wuhan University, Wuhan 430060, China. FAU - Yao, C AU - Yao C AD - Department of Otorhinolaryngology Head and Neck Surgery, Renmin Hospital, Wuhan University, Wuhan 430060, China. FAU - Kong, Y G AU - Kong YG AD - Department of Otorhinolaryngology Head and Neck Surgery, Renmin Hospital, Wuhan University, Wuhan 430060, China. FAU - Tao, Z Z AU - Tao ZZ AD - Department of Otorhinolaryngology Head and Neck Surgery, Renmin Hospital, Wuhan University, Wuhan 430060, China. LA - chi GR - 81770986/National Natural Science Foundation of China/ PT - Journal Article PL - China TA - Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi JT - Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery JID - 101247574 RN - 0 (Amphiregulin) SB - IM MH - Adolescent MH - Adult MH - Amphiregulin/*biosynthesis MH - Chronic Disease MH - Eosinophils/*metabolism MH - Female MH - Fibrosis/pathology MH - Humans MH - Male MH - Middle Aged MH - Nasal Mucosa/*metabolism/pathology/surgery MH - Nasal Polyps/*metabolism/pathology/surgery MH - Rhinitis/*metabolism/pathology/surgery MH - Sinusitis/*metabolism/pathology/surgery MH - Young Adult OTO - NOTNLM OT - Amphiregulin OT - Eosinophils OT - Nasal polyps EDAT- 2019/12/05 06:00 MHDA- 2019/12/25 06:00 CRDT- 2019/12/05 06:00 PHST- 2019/12/05 06:00 [entrez] PHST- 2019/12/05 06:00 [pubmed] PHST- 2019/12/25 06:00 [medline] AID - 10.3760/cma.j.issn.1673-0860.2019.11.009 [doi] PST - ppublish SO - Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2019 Nov 7;54(11):850-856. doi: 10.3760/cma.j.issn.1673-0860.2019.11.009.