PMID- 31799778
OWN - NLM
STAT- MEDLINE
DCOM- 20201102
LR  - 20231111
IS  - 1467-7652 (Electronic)
IS  - 1467-7644 (Print)
IS  - 1467-7644 (Linking)
VI  - 18
IP  - 6
DP  - 2020 Jun
TI  - Overexpression of a developing xylem cDNA library in transgenic poplar generates 
      high mutation rate specific to wood formation.
PG  - 1434-1443
LID - 10.1111/pbi.13309 [doi]
AB  - We investigated feasibility of the Full-length complementary DNA OvereXpression 
      (FOX) system as a mutagenesis approach in poplar, using developing xylem tissue. 
      The main goal was to assess the overall mutation rate and if the system will 
      increase instances of mutants affected in traits linked to the xylem tissue. 
      Indeed, we found a high mutation rate of 17.7%, whereas 80% of all mutants were 
      significantly affected in cellulose, lignin and/or hemicellulose. Cell wall 
      biosynthesis is a major process occurring during xylem development. Enrichment of 
      mutants affected in cell wall composition suggests that the tissue source for the 
      FOX library influenced the occurrence of mutants affected in a trait linked to 
      this tissue. Additionally, we found that FLcDNAs from mutants affected in cell 
      wall composition were homologous to genes known to be involved in cell wall 
      biosynthesis and most recovered FLcDNAs corresponded to genes whose native 
      expression was highest in xylem. We characterized in detail a mutant line with 
      increased diameter. The phenotype was caused by a poplar homolog of LONELY GUY 1 
      (LOG1), which encodes an enzyme in cytokinin biosynthesis and significantly 
      increased xylem proliferation. The causative role of LOG1 in the observed 
      phenotype was further reaffirmed by elevated cytokinin concentration in the 
      mutant and recapitulation overexpression experiment wherein multiple independent 
      lines phenocopied the original FOX mutant. Our experiments show that the FOX 
      approach can be efficiently used for gene discovery and molecular interrogation 
      of traits specific to woody perennial growth and development.
CI  - (c) 2019 The Authors. Plant Biotechnology Journal published by Society for 
      Experimental Biology and The Association of Applied Biologists and John Wiley & 
      Sons Ltd.
FAU - Rauschendorfer, James
AU  - Rauschendorfer J
AUID- ORCID: 0000-0002-1360-9419
AD  - College of Forest Resources and Environmental Science, Michigan Technological 
      University, Houghton, MI, USA.
FAU - Yordanov, Yordan
AU  - Yordanov Y
AUID- ORCID: 0000-0002-9371-2095
AD  - Department of Biology, Eastern Illinois University, Charleston, IL, USA.
FAU - Dobrev, Petre
AU  - Dobrev P
AUID- ORCID: 0000-0001-7412-6982
AD  - Institute of Experimental Botany, Czech Academy of Sciences, Prague, Czech 
      Republic.
FAU - Vankova, Radomira
AU  - Vankova R
AUID- ORCID: 0000-0001-9101-8844
AD  - Institute of Experimental Botany, Czech Academy of Sciences, Prague, Czech 
      Republic.
FAU - Sykes, Robert
AU  - Sykes R
AUID- ORCID: 0000-0002-2061-6625
AD  - Nuclear Materials Science, Los Alamos National Laboratory, Los Alamos, NM, USA.
FAU - Kulheim, Carsten
AU  - Kulheim C
AUID- ORCID: 0000-0002-0798-3324
AD  - College of Forest Resources and Environmental Science, Michigan Technological 
      University, Houghton, MI, USA.
FAU - Busov, Victor
AU  - Busov V
AUID- ORCID: 0000-0003-2344-1581
AD  - College of Forest Resources and Environmental Science, Michigan Technological 
      University, Houghton, MI, USA.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20191225
PL  - England
TA  - Plant Biotechnol J
JT  - Plant biotechnology journal
JID - 101201889
RN  - 0 (Plant Proteins)
RN  - 9005-53-2 (Lignin)
SB  - IM
MH  - Cell Wall/genetics/metabolism
MH  - Gene Expression Regulation, Plant/genetics
MH  - Gene Library
MH  - Lignin/metabolism
MH  - Mutation Rate
MH  - Plant Proteins/genetics/metabolism
MH  - *Populus/genetics/metabolism
MH  - *Wood/genetics/metabolism
MH  - Xylem/genetics/metabolism
PMC - PMC7207001
OTO - NOTNLM
OT  - fox hunting system
OT  - genetics
OT  - poplar
COIS- The authors declare no conflicts of interest.
EDAT- 2019/12/05 06:00
MHDA- 2020/11/03 06:00
PMCR- 2020/05/08
CRDT- 2019/12/05 06:00
PHST- 2019/07/24 00:00 [received]
PHST- 2019/12/16 00:00 [revised]
PHST- 2019/11/18 00:00 [accepted]
PHST- 2019/12/05 06:00 [pubmed]
PHST- 2020/11/03 06:00 [medline]
PHST- 2019/12/05 06:00 [entrez]
PHST- 2020/05/08 00:00 [pmc-release]
AID - PBI13309 [pii]
AID - 10.1111/pbi.13309 [doi]
PST - ppublish
SO  - Plant Biotechnol J. 2020 Jun;18(6):1434-1443. doi: 10.1111/pbi.13309. Epub 2019 
      Dec 25.