PMID- 31800162
OWN - NLM
STAT- MEDLINE
DCOM- 20210423
LR  - 20210423
IS  - 1582-4934 (Electronic)
IS  - 1582-1838 (Print)
IS  - 1582-1838 (Linking)
VI  - 24
IP  - 2
DP  - 2020 Jan
TI  - Importance of PNO1 for growth and survival of urinary bladder carcinoma: Role in 
      core-regulatory circuitry.
PG  - 1504-1515
LID - 10.1111/jcmm.14835 [doi]
AB  - PNO1 (partner of Nob1) was known as a RNA-binding protein in humans, and its 
      ortholog PNO1 was reported to participate ribosome and proteasome biogenesis in 
      yeasts. Yet there have been few studies about its functions in mammalian cells, 
      and so far its role in human cells has never been reported, especially in urinary 
      bladder cancer (UBC).We interrogated the cellular functions and clinical 
      significance of PNO1 in, and its molecular mechanism through microarrays and 
      bioinformatics analysis. Our findings support that PNO1 participates in promoting 
      proliferation and colonogenesis, while reducing apoptosis of UBC cells, and is 
      also predicted to be associated with the migration and metastasis of UBC PNO1 
      knockdown (KD) attenuated the tumorigenesis ability of UBC in mouse. PNO1 KD led 
      to the altered expression of 1543 genes that are involved in a number of 
      signalling pathways, biological functions and regulation networks. CD44, PTGS2, 
      cyclin D1, CDK1, IL-8, FRA1, as well as mTOR, p70 S6 kinase, p38 and Caspase-3 
      proteins were all down-regulated in PNO1 KD cells, suggesting the involvement of 
      PNO1 in inflammatory responses, cell cycle regulation, chemotaxis, cell growth 
      and proliferation, apoptosis, cell migration and invasiveness. This study will 
      enhance our understanding of the molecular mechanism of UBC and may eventually 
      provide novel targets for individualized cancer therapy.
CI  - (c) 2019 The Authors. Journal of Cellular and Molecular Medicine published by John 
      Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.
FAU - Lin, Chunhua
AU  - Lin C
AD  - Department of Urology, The Affiliated Yantai Yuhuangding Hospital of Qingdao 
      University, Yantai, China.
FAU - Yuan, Hejia
AU  - Yuan H
AD  - Department of Urology, The Affiliated Yantai Yuhuangding Hospital of Qingdao 
      University, Yantai, China.
FAU - Wang, Wenting
AU  - Wang W
AD  - The Central Laboratory, The Affiliated Yantai Yuhuangding Hospital of Qingdao 
      University, Yantai, China.
FAU - Zhu, Zhe
AU  - Zhu Z
AUID- ORCID: 0000-0002-5385-948X
AD  - Division of Regenerative Medicine, Department of Medicine, School of Medicine, 
      University of California, San Diego, La Jolla, CA, USA.
FAU - Lu, Youyi
AU  - Lu Y
AD  - Department of Urology, The Affiliated Yantai Yuhuangding Hospital of Qingdao 
      University, Yantai, China.
FAU - Wang, Jiahui
AU  - Wang J
AD  - The Central Laboratory, The Affiliated Yantai Yuhuangding Hospital of Qingdao 
      University, Yantai, China.
FAU - Feng, Fan
AU  - Feng F
AD  - Department of Urology, The Affiliated Yantai Yuhuangding Hospital of Qingdao 
      University, Yantai, China.
FAU - Wu, Jitao
AU  - Wu J
AD  - Department of Urology, The Affiliated Yantai Yuhuangding Hospital of Qingdao 
      University, Yantai, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191204
PL  - England
TA  - J Cell Mol Med
JT  - Journal of cellular and molecular medicine
JID - 101083777
RN  - 0 (PNO1 protein, human)
RN  - 0 (RNA-Binding Proteins)
SB  - IM
MH  - Animals
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics
MH  - Cell Survival/genetics
MH  - Down-Regulation/genetics
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Gene Knockdown Techniques
MH  - *Gene Regulatory Networks
MH  - Humans
MH  - Mice, Inbred BALB C
MH  - Mice, Nude
MH  - RNA-Binding Proteins/genetics/*metabolism
MH  - Up-Regulation/genetics
MH  - Urinary Bladder Neoplasms/*genetics/*pathology
PMC - PMC6991670
OTO - NOTNLM
OT  - PNO1
OT  - DNA microarray
OT  - bladder cancer
OT  - ingenuity pathway analysis
COIS- The authors confirm that there are no conflict of interests.
EDAT- 2019/12/05 06:00
MHDA- 2021/04/24 06:00
PMCR- 2020/01/01
CRDT- 2019/12/05 06:00
PHST- 2019/07/07 00:00 [received]
PHST- 2019/10/20 00:00 [accepted]
PHST- 2019/12/05 06:00 [pubmed]
PHST- 2021/04/24 06:00 [medline]
PHST- 2019/12/05 06:00 [entrez]
PHST- 2020/01/01 00:00 [pmc-release]
AID - JCMM14835 [pii]
AID - 10.1111/jcmm.14835 [doi]
PST - ppublish
SO  - J Cell Mol Med. 2020 Jan;24(2):1504-1515. doi: 10.1111/jcmm.14835. Epub 2019 Dec 
      4.