PMID- 31801411
OWN - NLM
STAT- MEDLINE
DCOM- 20210510
LR  - 20210510
IS  - 1557-8534 (Electronic)
IS  - 1547-3287 (Linking)
VI  - 29
IP  - 4
DP  - 2020 Feb 15
TI  - Exosomes from Bone Marrow Mesenchymal Stem Cells Can Alleviate Early Brain Injury 
      After Subarachnoid Hemorrhage Through miRNA129-5p-HMGB1 Pathway.
PG  - 212-221
LID - 10.1089/scd.2019.0206 [doi]
AB  - In this study, the roles of exosomes (Exo) from bone marrow mesenchymal stem 
      cells (BMSCs) in attenuating early brain injury (EBI) in rat brain after 
      subarachnoid hemorrhage (SAH) had been investigated. The male Sprague-Dawley rats 
      (300-350 g) were used to establish the SAH model using endovascular perforation 
      method. The animals were randomly divided into three groups: sham (n = 25), 
      SAH+PBS (n = 42), and SAH+Exo groups (n = 33). At 1 h after SAH, Exo or 
      phosphate-buffered saline (PBS) was administered by femoral vein injection. The 
      effects of Exo on the mortality, neurological function, brain water content, and 
      blood-brain barrier (BBB) were explored. Furthermore, the expressions of 
      miRNA129-5p and high-mobility group box 1 protein (HMGB1) after Exo treatment 
      were also detected. In addition, immunohistochemistry and western blot were 
      applied to investigate the mechanism of Exo's effects. The results indicated that 
      Exo could improve the neurological functions, reduce brain water content and 
      maintain BBB integrity after SAH. After Exo treatment, the expression of 
      miRNA129-5p was significantly increased, whereas the RNA level of HMGB1 was 
      decreased. The protein levels of proinflammatory and proapoptosis factors, such 
      as HMGB1, Toll-like receptor-4 (TLR4), tumor necrosis factor-alpha, and p53, were 
      increased after SAH, which were significantly declined after Exo application. The 
      results indicated that Exo from BMSCs could alleviate EBI after SAH through 
      miRNA129-5p's anti-inflammation and antiapoptosis effects through quenching the 
      activity of HMGB1-TLR4 pathway.
FAU - Xiong, Lili
AU  - Xiong L
AD  - Institute of Biomedical Engineering, West China School of Basic Medical Sciences 
      & Forensic Medicine, Sichuan University, Chengdu, China.
FAU - Sun, Linlin
AU  - Sun L
AD  - Department of Anatomy and Histology, School of Basic Medical Sciences, Peking 
      University, Beijing, China.
FAU - Zhang, Yixuan
AU  - Zhang Y
AD  - Department of Anatomy and Histology, School of Basic Medical Sciences, Peking 
      University, Beijing, China.
FAU - Peng, Jin
AU  - Peng J
AD  - Department of Histology and Embryology, West China School of Basic Medical 
      Sciences & Forensic Medicine, Sichuan University, Chengdu, China.
FAU - Yan, Junhao
AU  - Yan J
AD  - Department of Anatomy and Histology, School of Basic Medical Sciences, Peking 
      University, Beijing, China.
FAU - Liu, Xiaoheng
AU  - Liu X
AD  - Institute of Biomedical Engineering, West China School of Basic Medical Sciences 
      & Forensic Medicine, Sichuan University, Chengdu, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191230
PL  - United States
TA  - Stem Cells Dev
JT  - Stem cells and development
JID - 101197107
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (HMGB1 Protein)
RN  - 0 (Hbp1 protein, rat)
RN  - 0 (MIRN129 microRNA, rat)
RN  - 0 (MicroRNAs)
RN  - 0 (Tlr4 protein, rat)
RN  - 0 (Toll-Like Receptor 4)
SB  - IM
MH  - Animals
MH  - Blood-Brain Barrier/metabolism
MH  - Bone Marrow Cells/cytology/*metabolism
MH  - Brain Injuries/genetics/mortality/pathology/*therapy
MH  - Culture Media, Conditioned/chemistry/metabolism
MH  - Exosomes/metabolism/*transplantation
MH  - Gene Expression Regulation
MH  - HMGB1 Protein/*genetics/metabolism
MH  - Male
MH  - Mesenchymal Stem Cells/cytology/*metabolism
MH  - MicroRNAs/*genetics/metabolism
MH  - Permeability
MH  - Primary Cell Culture
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Signal Transduction
MH  - Subarachnoid Hemorrhage, Traumatic/genetics/mortality/pathology/*therapy
MH  - Survival Analysis
MH  - Toll-Like Receptor 4/genetics/metabolism
OTO - NOTNLM
OT  - blood-brain barrier
OT  - bone marrow mesenchymal stem cell
OT  - exosome
OT  - subarachnoid hemorrhage
EDAT- 2019/12/06 06:00
MHDA- 2021/05/11 06:00
CRDT- 2019/12/06 06:00
PHST- 2019/12/06 06:00 [pubmed]
PHST- 2021/05/11 06:00 [medline]
PHST- 2019/12/06 06:00 [entrez]
AID - 10.1089/scd.2019.0206 [doi]
PST - ppublish
SO  - Stem Cells Dev. 2020 Feb 15;29(4):212-221. doi: 10.1089/scd.2019.0206. Epub 2019 
      Dec 30.