PMID- 31801695 OWN - NLM STAT- MEDLINE DCOM- 20200406 LR - 20200408 IS - 1095-9947 (Electronic) IS - 1050-4648 (Linking) VI - 96 DP - 2020 Jan TI - Effects of dietary n-3 highly unsaturated fatty acids on growth, non-specific immunity, expression of some immune-related genes and resistance to Vibrio harveyi in hybrid grouper (female symbol Epinephelus fuscoguttatus x male symbol Epinephelus lanceolatu). PG - 86-96 LID - S1050-4648(19)31128-3 [pii] LID - 10.1016/j.fsi.2019.11.072 [doi] AB - This study was conducted to investigate the effects of dietary n-3 highly unsaturated fatty acids (n-3 HUFA) on growth performance, non-specific immunity, expression of some immune-related genes and resistance to Vibrio harveyi in juvenile hybrid grouper (female symbol Epinephelus fuscoguttatus x male symbol Epinephelus lanceolatu). Six isoproteic and isolipidic experimental diets were formulated with graded levels of n-3 HUFA (0.65, 1.00, 1.35, 1.70, 2.05 and 2.40% of dry matter, respectively), and the 0.65% group was used as control group. Each diet was randomly allocated to triplicate groups of fish in 1000 L fiberglass tank, and each tank was stocked with 40 fish (initial weight 12.06 +/- 0.01 g) for 8 weeks. Results showed that feed conversion ratio (FCR), survival rate (SR), hepatosomatic index (HSI) and condition factor (CF) were all not significantly affected by dietary n-3 HUFA levels (P > 0.05). Weight gain (WG) and specific growth rate (SGR) in 1.35% group were significantly higher than those in 2.40% group (P < 0.05). Crude lipid of body in 1.00% group was significantly lower than that in 1.70% and 2.40% groups (P < 0.05). Liver and muscle fatty acid profiles reflected that of diets. Before challenge with Vibrio harveyi, the activity of serum superoxide dismutase (SOD), catalase (CAT) and content of complement 3 (C3) in 1.35% and 1.70% groups significantly higher than those of control group (P < 0.05). After challenge with Vibrio harveyi, serum CAT, glutathione peroxidase (GSH-PX), lysozyme (LZM) and C3 all increased sharply, while SOD showed the opposite trend. Before challenge with Vibrio harveyi, the expression levels of intestine toll-like receptor 22 (TLR22) and myeloid differentiation factor 88 (MyD88) mRNA in 2.40% group were significantly increased, and the expression levels of tumour necrosis factor alpha (TNF-alpha) and interleukin 1beta (IL-1beta) mRNA in 2.05% group were significantly higher than those in 1.00% and 1.35% groups (P < 0.05). In addition, the TLR22 and IL-1beta mRNA levels in kidney of 1.70% group were significantly lower than those in control group (P < 0.05). After challenge with Vibrio harveyi, the expression level of MyD88 mRNA in intestine of 1.35% group was significantly higher than that in 1.00% group and from 1.70% to 2.40% groups (P < 0.05), while TNF-alpha and IL-1beta obtained minimum values in 1.70% group. In the kidney, the interleukin 10 (IL10) mRNA expression was significantly higher in 1.70% group than that in other groups, while the IL-1beta expression in 1.70% group was on the contrary and significantly lower than that in 2.40% group (P < 0.05). Results of this study suggested that moderate dietary n-3 HUFA (1.47%-1.70% HUFA) could improve the growth performance, non-specific immunity and inhibit the inflammatory response of hybrid grouper. CI - Copyright (c) 2019 Elsevier Ltd. All rights reserved. FAU - An, Wenqiang AU - An W AD - Laboratory of Aquatic Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang, Guangdong, 524088, China; Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Center of Guangdong Province, Guangdong, 524088, China. FAU - Dong, Xiaohui AU - Dong X AD - Laboratory of Aquatic Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang, Guangdong, 524088, China; Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Center of Guangdong Province, Guangdong, 524088, China. Electronic address: dongxiaohui2003@163.com. FAU - Tan, Beiping AU - Tan B AD - Laboratory of Aquatic Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang, Guangdong, 524088, China; Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Center of Guangdong Province, Guangdong, 524088, China. Electronic address: bptan@126.com. FAU - Yang, Qihui AU - Yang Q AD - Laboratory of Aquatic Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang, Guangdong, 524088, China; Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Center of Guangdong Province, Guangdong, 524088, China. FAU - Chi, Shuyan AU - Chi S AD - Laboratory of Aquatic Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang, Guangdong, 524088, China; Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Center of Guangdong Province, Guangdong, 524088, China. FAU - Zhang, Shuang AU - Zhang S AD - Laboratory of Aquatic Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang, Guangdong, 524088, China; Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Center of Guangdong Province, Guangdong, 524088, China. FAU - Liu, Hongyu AU - Liu H AD - Laboratory of Aquatic Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang, Guangdong, 524088, China; Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Center of Guangdong Province, Guangdong, 524088, China. FAU - Yang, Yuanzhi AU - Yang Y AD - Laboratory of Aquatic Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang, Guangdong, 524088, China; Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Center of Guangdong Province, Guangdong, 524088, China. LA - eng PT - Journal Article DEP - 20191201 PL - England TA - Fish Shellfish Immunol JT - Fish & shellfish immunology JID - 9505220 RN - 0 (Fatty Acids, Unsaturated) RN - Vibrio harveyi SB - IM MH - Animal Feed/analysis MH - Animals MH - Bass/genetics/growth & development/*immunology MH - Diet/veterinary MH - Dietary Supplements/analysis MH - *Disease Resistance/drug effects MH - Dose-Response Relationship, Drug MH - Fatty Acids, Unsaturated/administration & dosage/*metabolism MH - Fish Diseases MH - *Gene Expression/drug effects MH - *Immunity, Innate/drug effects MH - Random Allocation MH - Vibrio MH - Vibrio Infections OTO - NOTNLM OT - Hybrid grouper OT - Inflammatory related gene OT - Non-specific immunity OT - n-3 HUFA EDAT- 2019/12/06 06:00 MHDA- 2020/04/09 06:00 CRDT- 2019/12/06 06:00 PHST- 2019/05/31 00:00 [received] PHST- 2019/10/26 00:00 [revised] PHST- 2019/11/30 00:00 [accepted] PHST- 2019/12/06 06:00 [pubmed] PHST- 2020/04/09 06:00 [medline] PHST- 2019/12/06 06:00 [entrez] AID - S1050-4648(19)31128-3 [pii] AID - 10.1016/j.fsi.2019.11.072 [doi] PST - ppublish SO - Fish Shellfish Immunol. 2020 Jan;96:86-96. doi: 10.1016/j.fsi.2019.11.072. Epub 2019 Dec 1.