PMID- 31802255
OWN - NLM
STAT- MEDLINE
DCOM- 20201104
LR  - 20201104
IS  - 1863-2661 (Electronic)
IS  - 1863-2653 (Linking)
VI  - 225
IP  - 1
DP  - 2020 Jan
TI  - Structural, morphometric and immunohistochemical study of the rabbit accessory 
      olfactory bulb.
PG  - 203-226
LID - 10.1007/s00429-019-01997-4 [doi]
AB  - The accessory olfactory bulb (AOB) is the first neural integrative centre of the 
      vomeronasal system (VNS), which is associated primarily with the detection of 
      semiochemicals. Although the rabbit is used as a model for the study of 
      chemocommunication, these studies are hampered by the lack of knowledge regarding 
      the topography, lamination, and neurochemical properties of the rabbit AOB. To 
      fill this gap, we have employed histological stainings: lectin labelling with 
      Ulex europaeus (UEA-I), Bandeiraea simplicifolia (BSI-B4), and Lycopersicon 
      esculentum (LEA) agglutinins, and a range of immunohistochemical markers. Anti-G 
      proteins Galphai2/Galphao, not previously studied in the rabbit AOB, are expressed 
      following an antero-posterior zonal pattern. This places Lagomorpha among the 
      small groups of mammals that conserve a double-path vomeronasal reception. 
      Antibodies against olfactory marker protein (OMP), growth-associated protein-43 
      (GAP-43), glutaminase (GLS), microtubule-associated protein-2 (MAP-2), glial 
      fibrillary-acidic protein (GFAP), calbindin (CB), and calretinin (CR) 
      characterise the strata and the principal components of the BOA, demonstrating 
      several singular features of the rabbit AOB. This diversity is accentuated by the 
      presence of a unique organisation: four neuronal clusters in the accessory bulbar 
      white matter, two of them not previously characterised in any species (the gamma and 
      delta groups). Our morphometric study of the AOB has found significant differences 
      between sexes in the numerical density of principal cells, with larger values in 
      females, a pattern completely opposite to that found in rats. In summary, the 
      rabbit possesses a highly developed AOB, with many specific features that 
      highlight the significant role played by chemocommunication among this species.
FAU - Villamayor, Paula R
AU  - Villamayor PR
AUID- ORCID: 0000-0002-7361-7795
AD  - Department of Anatomy, Animal Production and Clinical Veterinary Sciences, 
      Faculty of Veterinary, University of Santiago de Compostela, Av Carballo Calero 
      s/n, 27002, Lugo, Spain.
FAU - Cifuentes, Jose Manuel
AU  - Cifuentes JM
AUID- ORCID: 0000-0001-9169-9651
AD  - Department of Anatomy, Animal Production and Clinical Veterinary Sciences, 
      Faculty of Veterinary, University of Santiago de Compostela, Av Carballo Calero 
      s/n, 27002, Lugo, Spain.
FAU - Quintela, Luis
AU  - Quintela L
AUID- ORCID: 0000-0002-0580-1216
AD  - Department of Animal Pathology, Faculty of Veterinary, University of Santiago de 
      Compostela, Lugo, Spain.
FAU - Barcia, Ramiro
AU  - Barcia R
AUID- ORCID: 0000-0002-7943-3452
AD  - Department of Biochemistry and Molecular Biology, Faculty of Veterinary, 
      University of Santiago de Compostela, Lugo, Spain.
FAU - Sanchez-Quinteiro, Pablo
AU  - Sanchez-Quinteiro P
AUID- ORCID: 0000-0002-9891-4817
AD  - Department of Anatomy, Animal Production and Clinical Veterinary Sciences, 
      Faculty of Veterinary, University of Santiago de Compostela, Av Carballo Calero 
      s/n, 27002, Lugo, Spain. pablo.sanchez@usc.es.
LA  - eng
GR  - 1551-8179/University of Santiago de Compostela/
PT  - Journal Article
DEP - 20191204
PL  - Germany
TA  - Brain Struct Funct
JT  - Brain structure & function
JID - 101282001
SB  - IM
MH  - Animals
MH  - Cell Count
MH  - Female
MH  - Immunohistochemistry
MH  - Male
MH  - Mice, Inbred BALB C
MH  - Neurons/*cytology
MH  - Olfactory Bulb/*cytology/metabolism
MH  - Rabbits
MH  - Sex Characteristics
MH  - Vomeronasal Organ/*cytology
OTO - NOTNLM
OT  - Accessory olfactory bulb
OT  - Immunohistochemistry
OT  - Morphometry
OT  - Rabbits
OT  - Sexual dimorphism
OT  - Vomeronasal system
EDAT- 2019/12/06 06:00
MHDA- 2020/11/05 06:00
CRDT- 2019/12/06 06:00
PHST- 2019/05/08 00:00 [received]
PHST- 2019/11/23 00:00 [accepted]
PHST- 2019/12/06 06:00 [pubmed]
PHST- 2020/11/05 06:00 [medline]
PHST- 2019/12/06 06:00 [entrez]
AID - 10.1007/s00429-019-01997-4 [pii]
AID - 10.1007/s00429-019-01997-4 [doi]
PST - ppublish
SO  - Brain Struct Funct. 2020 Jan;225(1):203-226. doi: 10.1007/s00429-019-01997-4. 
      Epub 2019 Dec 4.