PMID- 31804828 OWN - NLM STAT- MEDLINE DCOM- 20200123 LR - 20200123 IS - 1520-5118 (Electronic) IS - 0021-8561 (Linking) VI - 68 IP - 2 DP - 2020 Jan 15 TI - Competitive Bio-Barcode Immunoassay for Highly Sensitive Detection of Parathion Based on Bimetallic Nanozyme Catalysis. PG - 660-668 LID - 10.1021/acs.jafc.9b06125 [doi] AB - A competitive sensitive bio-barcode immunoassay based on bimetallic nanozyme (Au@Pt: gold@platinum) catalysis has been designed for the detection of the pesticide parathion. Gold nanoparticles (AuNPs) were modified with single-stranded thiol oligonucleotides (ssDNAs) and monoclonal antibodies (mAbs) to form AuNP probes; magnetic nanoparticles (MNPs) were coated with ovalbumin (OVA)-parathion haptens as MNP probes, and bimetallic nanozyme (Au@Pt) nanoparticles functionalized with the complementary thiolated ssDNA were used as Au@Pt probes. The Au@Pt probes reacted with the AuNP probes through complementary base pairing. Further, parathion competed with MNP probes to bind the mAbs on the AuNP probes. Finally, the complex system was separated by a magnetic field. The released Au@Pt probes catalyzed a chromogenic system consisting of teramethylbenzidine (TMB). The bimetallic nanozyme-based bio-barcode immunoassay was performed on rice, pear, apple, and cabbage samples to verify the feasibility of the method. The immunoassay exhibited a linear response from 0.01 to 40 mug.kg(-1), and the limit of detection (LOD) was 2.13 x 10(-3) mug.kg(-1). The recoveries and relative standard deviations (RSDs) ranged from 73.12 to 116.29% and 5.59 to 10.87%, respectively. The method was found to correlate well with data obtained by liquid chromatography-tandem mass spectrometry (LC-MS/MS). In conclusion, this method exhibits potential as a sensitive alternative method for the detection of a variety of pesticides, ensuring the safety of fruits and vegetables in agriculture. FAU - Chen, Ge AU - Chen G AD - Institute of Quality Standard and Testing Technology for Agro-Products, Key Laboratory of Agro-Product Quality and Safety, Chinese Academy of Agricultural Sciences; Key Laboratory of Agro-Product Quality and Safety , Ministry of Agriculture , Beijing 100081 , P.R. China. FAU - Jin, Maojun AU - Jin M AUID- ORCID: 0000-0002-9102-8572 AD - Institute of Quality Standard and Testing Technology for Agro-Products, Key Laboratory of Agro-Product Quality and Safety, Chinese Academy of Agricultural Sciences; Key Laboratory of Agro-Product Quality and Safety , Ministry of Agriculture , Beijing 100081 , P.R. China. FAU - Ma, Jun AU - Ma J AD - Institute of Quality Standard and Testing Technology for Agro-Products, Key Laboratory of Agro-Product Quality and Safety, Chinese Academy of Agricultural Sciences; Key Laboratory of Agro-Product Quality and Safety , Ministry of Agriculture , Beijing 100081 , P.R. China. FAU - Yan, Mengmeng AU - Yan M AD - Institute of Quality Standard and Testing Technology for Agro-Products, Key Laboratory of Agro-Product Quality and Safety, Chinese Academy of Agricultural Sciences; Key Laboratory of Agro-Product Quality and Safety , Ministry of Agriculture , Beijing 100081 , P.R. China. FAU - Cui, Xueyan AU - Cui X AD - Institute of Quality Standard and Testing Technology for Agro-Products, Key Laboratory of Agro-Product Quality and Safety, Chinese Academy of Agricultural Sciences; Key Laboratory of Agro-Product Quality and Safety , Ministry of Agriculture , Beijing 100081 , P.R. China. FAU - Wang, Yuanshang AU - Wang Y AD - Institute of Quality Standard and Testing Technology for Agro-Products, Key Laboratory of Agro-Product Quality and Safety, Chinese Academy of Agricultural Sciences; Key Laboratory of Agro-Product Quality and Safety , Ministry of Agriculture , Beijing 100081 , P.R. China. FAU - Zhang, Xiuyuan AU - Zhang X AD - Institute of Quality Standard and Testing Technology for Agro-Products, Key Laboratory of Agro-Product Quality and Safety, Chinese Academy of Agricultural Sciences; Key Laboratory of Agro-Product Quality and Safety , Ministry of Agriculture , Beijing 100081 , P.R. China. FAU - Li, Hui AU - Li H AD - Institute of Quality Standard and Testing Technology for Agro-Products, Key Laboratory of Agro-Product Quality and Safety, Chinese Academy of Agricultural Sciences; Key Laboratory of Agro-Product Quality and Safety , Ministry of Agriculture , Beijing 100081 , P.R. China. FAU - Zheng, Weijia AU - Zheng W AD - Institute of Quality Standard and Testing Technology for Agro-Products, Key Laboratory of Agro-Product Quality and Safety, Chinese Academy of Agricultural Sciences; Key Laboratory of Agro-Product Quality and Safety , Ministry of Agriculture , Beijing 100081 , P.R. China. FAU - Zhang, Yudan AU - Zhang Y AD - Institute of Quality Standard and Testing Technology for Agro-Products, Key Laboratory of Agro-Product Quality and Safety, Chinese Academy of Agricultural Sciences; Key Laboratory of Agro-Product Quality and Safety , Ministry of Agriculture , Beijing 100081 , P.R. China. FAU - Abd El-Aty, A M AU - Abd El-Aty AM AD - Department of Pharmacology, Faculty of Veterinary Medicine , Cairo University , 12211 Giza , Egypt. AD - Department of Medical Pharmacology, Medical Faculty , Ataturk University , 25240 Erzurum , Turkey. FAU - Hacimuftuoglu, Ahmet AU - Hacimuftuoglu A AD - Department of Medical Pharmacology, Medical Faculty , Ataturk University , 25240 Erzurum , Turkey. FAU - Wang, Jing AU - Wang J AUID- ORCID: 0000-0002-8961-9277 AD - Institute of Quality Standard and Testing Technology for Agro-Products, Key Laboratory of Agro-Product Quality and Safety, Chinese Academy of Agricultural Sciences; Key Laboratory of Agro-Product Quality and Safety , Ministry of Agriculture , Beijing 100081 , P.R. China. LA - eng PT - Evaluation Study PT - Journal Article DEP - 20200107 PL - United States TA - J Agric Food Chem JT - Journal of agricultural and food chemistry JID - 0374755 RN - 0 (Pesticides) RN - 49DFR088MY (Platinum) RN - 61G466064D (Parathion) RN - 7440-57-5 (Gold) SB - IM MH - Catalysis MH - Food Contamination/analysis MH - Fruit/chemistry MH - Gold/chemistry MH - Immunoassay/instrumentation/*methods MH - Limit of Detection MH - Metal Nanoparticles/chemistry MH - Parathion/*analysis MH - Pesticides/*analysis MH - Platinum/chemistry MH - Vegetables/chemistry OTO - NOTNLM OT - Au@Pt OT - AuNPs OT - bimetallic nanozyme OT - bio-barcode OT - immunoassay OT - parathion EDAT- 2019/12/06 06:00 MHDA- 2020/01/24 06:00 CRDT- 2019/12/06 06:00 PHST- 2019/12/06 06:00 [pubmed] PHST- 2020/01/24 06:00 [medline] PHST- 2019/12/06 06:00 [entrez] AID - 10.1021/acs.jafc.9b06125 [doi] PST - ppublish SO - J Agric Food Chem. 2020 Jan 15;68(2):660-668. doi: 10.1021/acs.jafc.9b06125. Epub 2020 Jan 7.