PMID- 31806325
OWN - NLM
STAT- MEDLINE
DCOM- 20200325
LR  - 20200325
IS  - 1879-1026 (Electronic)
IS  - 0048-9697 (Linking)
VI  - 708
DP  - 2020 Mar 15
TI  - Biodegradation and toxicity reduction of nonylphenol, 4-tert-octylphenol and 
      2,4-dichlorophenol by the ascomycetous fungus Thielavia sp HJ22: Identification 
      of fungal metabolites and proposal of a putative pathway.
PG  - 135129
LID - S0048-9697(19)35121-6 [pii]
LID - 10.1016/j.scitotenv.2019.135129 [doi]
AB  - Research on the biodegradation of emerging pollutants is gained great focus 
      regarding their detrimental effects on the environment and humans. The objective 
      of the present study was to evaluate the ability of the ascomycetes Thielavia sp 
      HJ22 to remove the phenolic xenobiotics nonylphenol (NP), 4-tert-octylphenol 
      (4-tert-OP) and 2,4-dichlorophenol (2,4-DCP). The strain showed efficient 
      degradation of NP and 4-tert-OP with 95% and 100% removal within 8 h of 
      incubation, respectively. A removal rate of 80% was observed with 2,4-DCP within 
      the same time. Under experimental conditions, the degradation of the tested 
      pollutants concomitantly increased with the laccase production and cytochrome 
      P450 monooxygenases inhibition. This study showed the involvement of laccase in 
      pollutants removal together with biosorption mechanisms. Additionally, results 
      demonstrated the participation of cytochrome P450 monooxygenase in the 
      elimination of 2,4-DCP. Liquid chromatography-mass spectrometry analysis revealed 
      several intermediates, mainly hydroxylated and oxidized compounds with less 
      harmful effects compared to the parent compounds. A decrease in the toxicity of 
      the identified metabolites was observed using Aliivibrio fischeri as 
      bioindicator. The metabolic pathways of degradation were proposed based on the 
      identified metabolites. The results point out the potential of Thielavia strains 
      in the degradation and detoxification of phenolic xenobiotics.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Mtibaa, Rim
AU  - Mtibaa R
AD  - Laboratory of Enzyme Engineering and Microbiology, Department of Biology, 
      National School of Engineers of Sfax, University of Sfax, BP <<1173>>, 3038 Sfax, 
      Tunisia. Electronic address: mtibaarim@gmail.com.
FAU - Ezzanad, Abdellah
AU  - Ezzanad A
AD  - Department of Organic Chemistry, University of Sciences, University of Cadiz, 
      Poligono Rio San Pedro 11510, Puerto Real, Cadiz, Spain.
FAU - Aranda, Elisabet
AU  - Aranda E
AD  - Institute of Water Research, Department of Microbiology, University of Granada, 
      Ramon y Cajal 4, E-18071 Granada, Spain.
FAU - Pozo, Clementina
AU  - Pozo C
AD  - Institute of Water Research, Department of Microbiology, University of Granada, 
      Ramon y Cajal 4, E-18071 Granada, Spain.
FAU - Ghariani, Bouthaina
AU  - Ghariani B
AD  - Laboratory of Enzyme Engineering and Microbiology, Department of Biology, 
      National School of Engineers of Sfax, University of Sfax, BP <<1173>>, 3038 Sfax, 
      Tunisia.
FAU - Moraga, Javier
AU  - Moraga J
AD  - Department of Organic Chemistry, University of Sciences, University of Cadiz, 
      Poligono Rio San Pedro 11510, Puerto Real, Cadiz, Spain.
FAU - Nasri, Moncef
AU  - Nasri M
AD  - Laboratory of Enzyme Engineering and Microbiology, Department of Biology, 
      National School of Engineers of Sfax, University of Sfax, BP <<1173>>, 3038 Sfax, 
      Tunisia.
FAU - Manuel Cantoral, Jesus
AU  - Manuel Cantoral J
AD  - Department of Biomedicine, Biotechnology and Public Health, Facultad de Ciencias 
      del Mar y Ambientales, University of Cadiz, Poligono Rio San Pedro 11510 Puerto 
      Real, Cadiz, Spain.
FAU - Garrido, Carlos
AU  - Garrido C
AD  - Department of Biomedicine, Biotechnology and Public Health, Facultad de Ciencias 
      del Mar y Ambientales, University of Cadiz, Poligono Rio San Pedro 11510 Puerto 
      Real, Cadiz, Spain.
FAU - Mechichi, Tahar
AU  - Mechichi T
AD  - Laboratory of Biochemistry and Enzymatic Engineering of Lipases, National School 
      of Engineers of Sfax, University of Sfax, BP <<1173>>, 3038 Sfax, Tunisia.
LA  - eng
PT  - Journal Article
DEP - 20191121
PL  - Netherlands
TA  - Sci Total Environ
JT  - The Science of the total environment
JID - 0330500
RN  - 0 (Chlorophenols)
RN  - 0 (Phenols)
RN  - 79F6A2ILP5 (nonylphenol)
RN  - IOY9FVU3J3 (4-tert-octylphenol)
RN  - R669TG1950 (2,4-dichlorophenol)
SB  - IM
MH  - *Biodegradation, Environmental
MH  - Chlorophenols
MH  - Phenols
OTO - NOTNLM
OT  - Acute toxicity
OT  - Biodegradation
OT  - Cytochrome P450s
OT  - Laccase
OT  - Metabolic pathway
OT  - Thielavia sp
EDAT- 2019/12/07 06:00
MHDA- 2020/03/26 06:00
CRDT- 2019/12/07 06:00
PHST- 2019/07/06 00:00 [received]
PHST- 2019/09/17 00:00 [revised]
PHST- 2019/10/21 00:00 [accepted]
PHST- 2019/12/07 06:00 [pubmed]
PHST- 2020/03/26 06:00 [medline]
PHST- 2019/12/07 06:00 [entrez]
AID - S0048-9697(19)35121-6 [pii]
AID - 10.1016/j.scitotenv.2019.135129 [doi]
PST - ppublish
SO  - Sci Total Environ. 2020 Mar 15;708:135129. doi: 10.1016/j.scitotenv.2019.135129. 
      Epub 2019 Nov 21.